PECULIARITIES OF CYTOKINE PROFILE CHANGES UNDER THE CONDITIONS OF EXPERIMENTAL PERIODONTITIS AND IMMOBILIZATION STRESS FORMATION AND ITS PHARMACOLOGICAL CORRECTION

Authors:

Abstract:

The study was devoted to establishing the character of the disturbances content level of TNF-α, IL-6 and IL-10 in the serum of guinea pigs under the conditions of experimental periodontitis and immobilization stress and to evaluate the effectiveness of the thiocetam use. Experimental studies were performed on 50 guinea pigs, which were divided into three groups. The concentration of IL-6, IL-10 and TNF-α in the blood serum was determined. Experimental combined pathology was accompanied by a gradual and significant increase in the blood content of TNF-α and IL-6 with the highest degree of manifestation on day 15 of the experiment and significant depletion of anti-inflammatory cytokine, indicating a significant predominance of proinflammatory activity of the cytokine profile. The administration of thiocetam had a positive effect on the cytokine profile and contributed to a decrease in the proinflammatory cytokine link and a significant increase in the level of interleukin-10 under conditions of experimental periodontitis and immobilization stress.

Keywords:

experimental periodontitis immobilization stress tumor necrosis factor-α interleukin-6 interleukin-10 thiocetam

References:

1. Sikirynska DO. Vplyv cherepno-skeletnoyi travmy, uskladnenoyi krovovtratoyu, na funktsionalno-morfolohichni porushennya pechinky u shchuriv z riznoyu stiykistyu do hipoksiyi ta yikh korektsiya. [Abstract of a thesis]. Ternopil, UA; Ternopil National Medical University named after I. Gorbachevsky of the Ministry of Health of Ukraine;2021. 22 p. [in Ukrainian]
2. Barbato L, Francioni E, Bianchi M, Mascitelli E, Marco LB, Tonelli DP. Periodontitis and bone metabolism. Clinical Cases in Mineral and Bone Metabolism. 2015;12(2):174. DOI: 10.11138/ccmbm/2015.12.2.174.
3. Demkovych A, Bondarenko Y, Hasiuk PA. Oxidative modification of proteins in the process of experimental periodontitis development. Interventional Medicine and Applied Science. 2017;9(4):218–21. doi: 10.1556/1646.9.2017.28.
4. Hacker S, Reichel T, Hecksteden A, Weyh C, Gebhardt K, Pfeiffer M, et al. Recovery-stress response of blood-based biomarkers. International Journal of Environmental Research and Public Health. 2021;18(11):5776. doi: 10.3390/ijerph18115776.
5. Horodetskyy O. The role of prooxidative and antioxidant processes in periodontal tissue in the mechanisms of formation of adrenalin damage of myocardium and experimental periodontitis and their correction with Corvitin. Journal of Education, health and sport. 2019;9(11):269–276. doi: 10.12775/JEHS.2019.09.11.024.
6. Kinane DF, Stathopoulou PG, Papapanou PN. Periodontal diseases. Nature reviews Disease primers. 2017;3(1):1–4. doi: 10.1038/nrdp.2017.38.
7. Lys O, Regeda M, Semenciv N, Regeda-Furdychko M, Regeda S. Peculiarities of disorders of nitrogen oxide system in the blood at adrenalin-inducedmyocardial injury in conditions of immobilization stress and their correction by l-arginine. ScienceRise: Pharmaceutical Science. 2021 (4(32), 24–28. doi: https://doi.org/10.15587/2519-4852.2021.238320
8. Manukyan AL, Grigoryan AS, Hunanyan LS, Harutyunyan HA, Manukyan MV, Mkrtchyan VS, et.al. Alfa2-adrenoblockers attenuate the elevated plasma cholesterol, anxiety levels and restore impaired spatial memory of rats under the chronic noise exposure. Science of The Total Environment. 2020;740:140390. doi: 10.1016/j.scitotenv.2020.140390.
9. Owen R, Reilly GC. In vitro models of bone remodelling and associated disorders. Frontiers in bioengineering and biotechnology. 2018;6:134. doi: 10.3389/fbioe.2018.00134.
10. Petit C, Anadon‐Rosinach V, Rettig L, Schmidt‐Mutter C, Tuzin N, Davideau JL, et.al. Influence of psychological stress on non‐surgical periodontal treatment outcomes in patients with severe chronic periodontitis. Journal of periodontology. 2021;92(2):186–95. doi: 10.1002/JPER.20-0105.
11. Rinčić G, Gaćina P, Virović Jukić L, Rinčić N, Božić D, Badovinac A. Association between Periodontitis and Liver Disease. Acta Clinica Croatica. 2021;60(3):510–8. doi: 10.20471/acc.2021.60.03.22.
12. Tan J, Zhang M, Hai Z, Wu C, Lin J, Kuang W, et al. Sustained release of two bioactive factors from supramolecular hydrogel promotes periodontal bone regeneration. ACS Nano. 2019;13(5):5616–22. doi: 10.1021/acsnano.9b00788.
13. Trivedi S, Lal N. Antioxidant enzymes in periodontitis. Journal of Oral biology and Craniofacial Research. 2017;7(1):54–7. doi: 10.1016/j.jobcr.2016.08.001.
14. Usui M, Onizuka S, Sato T, Kokabu S, Ariyoshi W, Nakashima K. Mechanism of alveolar bone destruction in periodontitis – Periodontal bacteria and inflammation. Japanese Dental Science Review. 2021;57:201–8. doi: 10.1016/j.jdsr.2021.09.005.
15. Yelinska AM, Kostenko VO. Indicators of oxidative-nitrosative stress in periodontal tissues of rats exposed to local irritation of gums during lipopolysaccharide-induced systemic inflammatory response. World of Medicine and Biology. 2019;3(69):184–187. DOI: 10.26724/2079-8334-2019-3-69-184-187.

Publication:

«World of Medicine and Biology» Vol. 19 No. 84 (2023) , с. 225-229
УДК 616.155.3-097.37.616.314.18-002.4-085

DOI:

https://doi.org/10.26724/2079-8334-2023-2-84-225-229