THYROID GLAND PARENCHYMA MORPHOLOGICAL ABNORMALITIES IN RATS ON THE THIRD DAY AFTER SKIN THERMAL BURNING

Authors:

Abstract:

The purpose of the study was to establish thyroid gland histological and ultrastructural changes of experimental animals 3 days after the thermal skin injury under physiological 0.9 % NaCl solution administration. Intrathyroid hemodynamic changes, the stromal component structure alterations and destructive processes in the thyroid gland morpho-functional unit - the follicle - were detected on the microscopic level three days after the animals’ skin experimental thermal injury under the physiological 0.9 % NaCl solution administration. Thyroid gland capsule and stroma throughout this period of the trials were characterized by collagen fibers swelling and focal leukocyte infiltration with segmented neutrophils predominance. Thyroid hemodynamics disturbances were manifested by arterial and venous plethora, stasis in the arterial vessels of the organ and components of the microcirculatory bed. Follicular cells contained a small nucleus with shallow karyolemа invaginations, marginally located heterochromatin and small lumps of it dispersed throughout the karyoplasm. Experimental animals’ thyroid gland hemocapillaries were characterized by both basement membrane components and the endothelial layer swelling as well as stasis of the lumen. Endotheliocytes contained nuclei with heterochromatin peripheral localization. The third day after a skin burn under the physiological 0.9 % NaCl solution administration resulted in certain alterations in the structure of the vascular component of the gland; its stroma and parenchyma were established at the microscopic and submicroscopic level, which could lead to a violation of the secretory cycle of follicular cells and, as a result, to an imbalance in the synthesis and excretion of thyroid hormones. These pathomorphological intrathyroid disorders indicate these micro-changes possible reversibility which is important in terms of such a contingent of patients’ pharmacotherapy.

Keywords:

thyroid gland parenchyma burning injury morphological disturbances hematoxylin-eosin follicle thyrocyte capillary stasis

References:

1. Bagrii MM, Dibrova VA, Popadynets OG, Gryshhuk MI. Metodyky morfologichnykh doslidzhen. Vinnytsia: Nova knyga. 2016; 328 [in Ukrainian]
2. Voyenno-polyova khirurhiya: pidruchnyk. Red. Ja.L. Zaruckyi, V.Ja. Bilyj. Kyiv : FENІKS, 2018: 544 [in Ukrainian]
3. Horalskyy LP, Khomych VT, Kononskyy OI. Osnovy histolohichnoyi tekhniky i morfofunktsionalni metody doslidzhen u normi ta pry patolohiyi. Zhytomyr : ZhNAEU, 2019: 286 [in Ukrainian]
4. Nebesna ZM, Yeroshenko GA. Histolohichni ta histokhimichni zminy lehen pry eksperymentalniy termichniy travmi. Svit medytsyny ta biolohiyi. 2015; 2(49) :106–110 [in Ukrainian]
5. Strelchenko YuI, Zyablitsev SV, Yelskyy VM. Patofiziolohichni vzayemozvyazky hipofizarno-tyreoyidnoyi ta hipofizarno-nadnyrkovoyi system pid vplyvom polyaryzovanoho svitla v shchuriv iz dozovanym opikom vidkrytym polumyam. Clinical and experimental pathology. 2012; 11(2–3) :156–158 [in Ukrainian]
6. Chernyakova HM, Minukhin VV, Voronin EP. Suchasnyy pohlyad na mistseve likuvannya opikiv z infektsiynym komponentom. Herald of problems of biology and medicine. 2016; 4(133) :68–72 [in Ukrainian].
7. Bahemia IA, Muganza A, oore R, Sahid F, Menezes CN. Microbiology and antibiotic resistance in severe burns patients: a 5 year review in an adult burns unit. Burns 2015; 41(7): 1536–1542, https://doi.org/10.1016/j. burns.2015.05.007.
8. Dzevulska ІV, Kovalchuk ОІ, Cherkasov EV, Majewskyi ОYe, Shevchuk YuG, Pastukhova VA, Kyselova TM. Influence of lactoproteinum solution with sorbitol on DNA content of cells of endocrine glands on the background of skin burn in rats. World of Medicine and Biology. 2017; 2(64):33–39. doi:10.26724/2079-8334-2018-2-64-33-39.
9. Gunas I, Dovgan I, Masur O. Method of thermal burn trauma correction by means of cryoinfluence. Abstracts are presented in zusammen mit der Polish Anatomical Society with the participation of the Association des Anatomistes Verhandlungen der Anatomischen Gesellschaft, Olsztyn. Jena – Munchen Der Urban & Fischer Verlag, 1997: 105.
10. Hwang W, Yoon SS. Virulence characteristics and an action mode of antibiotic resistance in multidrug-resistant Pseudomonas aeruginosa. Sci Rep 2019; 9(1): 487, https://doi. org/10.1038/s41598-018-37422-9.
11. Leontyev АE, Pavlenko IV, Kovalishena ОV, Saperkin NV, Tulupov АА, Beschastnov VV Application of Phagotherapy in the Treatment of Burn Patients (Review). Sovrem Tekhnologii Med. 2021;12(3) :95–103. doi: 10.17691/stm2020.12.3.12.
12. Plichta JK, Holmes CJ, Gamelli RL, Radek KA. Local burn injury promotes defects in the epidermal lipid and antimicrobial peptide barriers in human autograft skin and burn margin: implications for burn wound healing and graft survival. J Burn Care Res. 2017;38(1) :212–226. https://doi. org/10.1097/bcr.0000000000000357.
13. Tiron OI. Features of morphological changes in the thyroid gland of white male rats 1 day after thermal trauma of the skin on the background of the introduction of 0.9 % NaCl solution. Biomedical and Biosocial Anthropology, 2019; 37: 55–59. DOI: https://doi.org/10.31393/bba37-2019-09
14. Tiron O.I., Stetsenko A.V., Yatsyna O.I., Zayats L.M., Kolotvin A.O., Shumilina K.S. The morphological changes of the white rats’ thyroid gland 21 days after experimental thermal burn injury under NaCl systemic administration // World of Medicine and Biology. 2022; 2(80) :237–241. DOI 10.26724/2079-8334-2022-2-80-237-241
15. Williams FN, Herndon DN. Metabolic and Endocrine Considerations After Burn Injury. Clin Plast Surg. 2017;44(3):541–553. doi: 10.1016/j.cps.2017.02.013.

Publication:

«World of Medicine and Biology» Vol. 18 No. 81 (2022) , с. 231-235
УДК 616.441:599.323.4:615.459

DOI:

https://doi.org/10.26724/2079-8334-2022-3-81-231-235