IMMUNOHISTOCHEMICAL CHARACTERISTICS OF THYROID GLAND CHANGES IN PATIENTS WITH AUTOIMMUNE THYROIDITIS

Authors:

Abstract:

The purpose of the study was to perform an immunohistochemical study of autoimmune changes in the thyroid gland in autoimmune thyroiditis patients who received various treatment methods. In the main group, immunohistochemical studies were performed on 30 patients. The control group consisted of six patients in whom the histochemical examination was performed postmortem. Statistically significant differences were found between the forms of autoimmune thyroiditis according to Ki–67(+) (F=1428.687, p<0.050). According to the specific amount of Ki–67(+), p53(+), and thyroglobulins, there is a statistical difference between forms of autoimmune thyroiditis in cells. A correct assessment of the proliferative activity of the thyroid gland tissue during immunohistochemical studies with the determination of biomarkers Ki–67(+), p53(+) and thyroglobulin may indicate the preservation of the apoptosis mechanism, which will allow to predict the risk of recurrence of the disease and the malignancy of nodular forms of autoimmune thyroiditis.

Keywords:

autoimmune thyroiditis thyroglobulin proliferative activity biomarker immunohistochemical studies

References:

1. Abrosimov AY. Novaa mezhdunarodnaya gistologicheskaya klassifikatsiya opukholey shchytovidnoy zhelezy. Arkhiv patologii. 2018;80(1):37–45. doi.org/10.17116/patol201880137-45. [in Russian]
2. Butolina KM, Lyalikov SA, Basinsky VA, Shtabinskaya TT, Marshalek A. Immunogistokhimicheskaya kharakteristika fenotipa vnutritireoidnykh limfotsitov pri autoimmunnom tireoidite. Vestnik Grodnenskogo gosudarstvennogo meditsinskogo universiteta. 2016;(2):55–58. [in Russian]
3. Muravleva AV. Autoimunnyy tyreoyidyt ta yoho vuzlovi formy v strukturi suchasnoyi klinichnoyi tyreoyidopatiyi. Svit medytsyny ta biolohiyi. 2014;47(4):50–52. [in Ukrainian]
4. Jokisch F, Kleinlein I, Haller B, Seehaus T, Fuerst H, Kremer M. A small subgroup of Hashimoto’s thyroiditis is associated with IgG4–related disease. Virchows Archiv. 2016;468(3):321–327.doi.org/10.1007/s00428-015-1893-6
5. Kang S, Kang J, Shen H, Wu N. Advances in regulatory B cells in autoimmune thyroid diseases. Int. Immunopharmacol. 2021; 96:107770. doi. 10.1016/j.intimp.2021.107770.
6. Kristensen B, Hegedüs L, Lundy SK, Brimnes MK, Smith TJ, Nielsen CH. Characterization of regulatory B cells in Graves' disease and Hashimoto's thyroiditis. PLoS One. 2015;10(5): e0127949. doi. 10.1371/journal.pone.01279492.
7. Kholová I, Kalfert D, Lintusaari J, Rajakorpi E, Ludvíková M. Follicular epithelial dysplasia as Hashimoto thyroiditis – related atypia: a series of 91 specimens. Endocrine Pathology. 2021;32(3):368–374. doi. 10.1007/s12022-021-09679-w.
8. Luty J, Ruckemann-Dziurdzińska K, Witkowski JM, Bryl E. Immunological aspects of autoimmune thyroid disease – Complex interplay between cells and cytokines. Cytokine. 2019;(116):128–133. doi.10.1016/j.cyto.2019.01.003.
9. Pyzik A, Grywalska E, Matyjaszek-Matuszek B, Roliński J. Immune disorders in Hashimoto's thyroiditis: what do we know so far? Journal of Immunology Research. 2015;(1):1–8. doi. 10.1155/2015/979167.
10. Ralli M, Angeletti D, Fiore M, D'Aguanno V, Lambiase A, Artico M et al. Hashimoto's thyroiditis: An update on pathogenic mechanisms, diagnostic protocols, therapeutic strategies, and potential malignant transformation. Autoimmun Rev. 2020;19(10):102649. doi.10.1016/j.autrev.2020.102649.
11. Raess PW, Habashi A, El Rassi E, Milas M, Sauer DA, Troxell ML. Overlapping morphologic and immunohistochemical features of Hashimoto thyroiditis and IgG4–Related thyroid disease. Endocr Pathol. 2015;26(2):170–177. doi.org/10.1007/s12022-015-9368-5
12. Rency K, Santha S, Dain CP. Morphological and immunohistochemical approaches to the diagnosis of Hashimoto thyroiditis and mucosa associated lymphoid tissue lymphomas: An audit. Rare Tumors. 2020;12. doi.org/10.1177/20363613209725601.
13. Sheremet MI, Sydorchuk LP, Shidlovskyi VO, Desiateryk VI, Kovalenko AE, Shevchenko SI et al. The activity of proliferation and apoptosis of thyrocytes in the thyroid tissue of patients of nodular goiter with autoimmune thyroiditis considering the polymorphism of the BCL–2 (RS17759659), CTLA–4 (RS231775), APO–1/FAS (RS2234767) genes. Biointerface Research in Applied Chemistry. 2020;10(2):5201–5208. doi.org/10.33263/BRIAC102.201208
14. Yoo WS, Chung HK. Recent advances in autoimmune thyroid diseases. Endocrinol. Metab. 2016;31(3):379–385. doi. 10.3803/EnM.2016.31.3.379.
15. Yu Y, Liu J, Yu N, Zhang Y, Zhang S, Li T et al. IgG4 immunohistochemistry in Riedel's thyroiditis and recommended diagnostic criteria: case series and literature review. Wedge Endocrinol (Oxf). 2021;94(5):851–857. doi. 10.1111/сен.14390.

Publication:

«World of Medicine and Biology» Vol. 19 No. 84 (2023) , с. 139-143
УДК 616.44–089

DOI:

https://doi.org/10.26724/2079-8334-2023-2-84-139-143