THE ROLE OF SERUM AND FOLLICULAR FLUID CYTOKINES IN SUCCESSFUL RESULTS OF IN VITRO FERTILIZATION PROCEDURE

Authors:

Abstract:

The purpose of the study was to find out the association between cytokines of serum and follicular fluid and successful results of an in vitro fertilization procedure. A total of 131 patients were included in the study. They were grouped based on the outcome of the in vitro fertilization procedure. The cytokines in the serum and follicular aspiration fluid obtained during the process of oocyte pick-up were measured. There was no significant difference in cytokines levels in serum and follicular fluid in patients with and without success results of in vitro fertilization procedure. IL-1β (p=0.851), IL-6 (p=0.994) in follicular aspiration fluid and IL-1β (p=0.266), IL-5 (p=0.365) and IL-6 (p=0.377) were different in serum, but the differences were not statistically significant (p˃0.05). Our study suggests the nonsignificant association between some cytokines in serum and follicular fluid and successful results of in vitro fertilization procedure, but it is necessary to conduct the further studies for more detail results.

Keywords:

pregnancy reproductive technologies immunological markers female infertility

References:

1. Alhilali MJS, Parham A, Attaranzadeh A, Amirian M, Azizzadeh M. IL-5 in follicular fluid as a negative predictor of the intracytoplasmic sperm injection outcome. Cytokine. 2019 Jan; 113:265–271. doi: 10.1016/j.cyto.2018.07.016.
2. Amjadi F, Zandieh Z, Mehdizadeh M, Aghajanpour S, Raoufi E, Aghamajidi A, et al. The Uterine Immunological Changes May Be Responsible for Repeated Implantation Failure. J Reprod Immunol. 2020; 138:103080. doi: 10.1016/j.jri.2020.103080
3. Guo L, Guo A, Yang F, Li L, Yan J, Deng X, et al. Alterations of Cytokine Profiles in Patients With Recurrent Implantation Failure. Front Endocrinol (Lausanne). 2022 Jul 11; 13:949123. doi: 10.3389/fendo.2022.949123.
4. Hercor M, Anciaux M, Denanglaire S, Debuisson D, Leo O, Andris F. Antigen-Presenting Cell-Derived IL-6 Restricts the Expression of GATA3 and IL-4 by Follicular Helper T Cells. J Leukoc Biol. 2017; 101:5–14. doi: 10.1189/jlb.1HI1115-511R
5. Khadem N, Mansoori M, Attaran M, Attaranzadeh A, Zohdi E. Association of IL-1 and TNF-α Levels in Endometrial Secretion and Success of Embryo Transfer in IVF/ICSI Cycles. Int J Fertil Steril. 2019 Oct;13(3):236–239. doi: 10.22074/ijfs.2019.5668.
6. Kreines FM, Nasioudis D, Minis E, Irani M, Witkin SS, Spandorfer S. IL-1β predicts IVF outcome: a prospective study. J Assist Reprod Genet. 2018 Nov;35(11):2031–2035. doi: 10.1007/s10815-018-1296-0.
7. Likhachov LV, Vashchenko VL, Taranovska OO, Dobrovolska LM, Makarov OG. Changes in cell-mediated and humoral immunity indices and levels of pro- and anti-inflammatory cytokines in cervical mucus in pregnant women of high risk for preeclampsia. World of Medicine and Biology. 2021; 2 (76):79–83. doi: http://dx.doi.org/10.26724/2079-8334-2021-2-76-79-83
8. Madziyire MG, Magwali TL, Chikwasha V, Mhlanga T. The causes of infertility in women presenting to gynaecology clinics in Harare, Zimbabwe; a cross sectional study. Fertil Res Pract. 2021 Jan 5;7(1):1. doi: 10.1186/s40738-020-00093-0.
9. Mascarenhas MN, Flaxman SR, Boerma T, Vanderpoel S, Stevens GA. National, regional, and global trends in infertility prevalence since 1990: a systematic analysis of 277 health surveys. PLoS Med. 2012;9(12): e1001356. doi: 10.1371/journal.pmed.1001356.
10. McGeachy MJ, Cua DJ, Gaffen SL. The IL-17 Family of Cytokines in Health and Disease. Immunity. 2019 Apr 16;50(4):892-906. doi: 10.1016/j.immuni.2019.03.021.
11. Sanderman EA, Willis SK, Wise LA. Female dietary patterns and outcomes of in vitro fertilization (IVF): a systematic literature review. Nutr J. 2022 Jan 18; 21(1):5. doi: 10.1186/s12937-021-00757-7.
12. Sequeira K, Espejel-Núñez A, Vega-Hernández E, Molina-Hernández A, Grether-González P. An increase in IL-1β concentrations in embryo culture-conditioned media obtained by in vitro fertilization on day 3 is related to successful implantation. J Assist Reprod Genet. 2015 Nov;32(11):1623-7. doi: 10.1007/s10815-015-0573-4.
13. Stojanovic Gavrilovic AZ, Cekovic JM, Parandilovic AZ, Nikolov AB, Sazdanovic PS, Velickovic AM, et al. IL-6 of follicular fluid and outcome of in vitro fertilization. Medicine (Baltimore). 2022 Jul 22;101(29): e29624. doi: 10.1097/MD.0000000000029624
14. WHO. Infertility is a global public health issue. WHO. World Health Organization; [cited 2020 May 31]. Available from: http://www.who.int/reproductivehealth/topics/infertility/perspective/en/
15. Zhang Y, Wang Y, Li MQ, Duan J, Fan DX, Jin LP. IL-25 promotes Th2 bias by upregulating IL-4 and IL-10 expression of decidual γδT cells in early pregnancy. Exp Ther Med. 2018 Feb;15(2):1855–1862. doi: 10.3892/etm.2017.5638.

Publication:

«World of Medicine and Biology» Vol. 19 No. 84 (2023) , с. 144-147
УДК 618.256:616-036.8

DOI:

https://doi.org/10.26724/2079-8334-2023-2-84-144-147