OXIDATIVE STATUS AND STATE OF ERYTHROCYTE MEMBRANES IN PATIENTS WITH CONTROLLED AND TRUE RESISTANT ARTERIAL HYPERTENSION

Authors:

Abstract:

Today, arterial hypertension is a global health problem in the whole world. The purpose of the study is to assess the oxidative status and the structural condition of erythrocyte membranes in patients with controlled and true resistant arterial hypertension. In patients, a deterioration in the oxidative status in the development of oxidative stress is observed as evidenced by a significant (p<0.05) increase in myeloperoxidase activity, accumulation of lipid and protein oxidation products, decreased antioxidant enzyme activity and reduced glutathione content. Structural and dynamic changes in the membranes of erythrocytes in these patients by the method of spin probes using the nitroxyl radical AdTEMPO and the damage to the structural organization of the lipid layer and proteins integrated in it as well as antioxidant system of erythrocytes were demonstrated. The obtained results may indicate an impairment of the structural organization of the lipid layer and its integrated proteins.

Keywords:

controlled and true resistant arterial hypertension oxidative status erythrocyte membranes electron paramagnetic resonance

References:

1. Zhdan VM, Kitura OIe, Kitura YeM, Babanina MIu, Tkachenko MV. Arterialna hipertenziya i sertseva nedostatnist u zahalnolikarskiy praktytsi. Simeyna medytsyna. 2020; 1-2(87-88): 81-84. [in Ukrainian]
2. Kopytova TV. Issledovaniye sorbtsionnoy sposobnosti membran eritrotsitov dlya otsenki kharaktera endogennoy intoksikatsii pri dermatozakh. Clin Lab Diagnost. 2006; 1: 18–9. [in Russian]
3. Malynovska IE, Kuchmenko OB., Shumakov VO, Tereshchenko NM, Mkhitaryan LS, Ievstratova IN, et al. Kliniko-biokhimichni osoblyvosti na riznykh etapakh kardiolohichnoyi reabilitatsiyi v patsiyentiv, yaki perenesly infarkt miokarda. Ukrainian Journal of Cardiology. 2018. 2: 14-23. [in Ukrainian]
4. Melnychuk SD, Khyzhniak SV, Nardid OA, Cherkashyna YaO, Morozova VS, Riepina SV. Otsinka aktyvnosti dykhalnoho lantsiuha mitokhondriyi hepatotsytiv ta kardiomiotsytiv shchuriv za shtuchnoho hipobiozu metodom spinovoho zondu. Biolohiia tvaryn. 2015; 17(3): 97–103. [in Ukrainian]
5. Melnyk AK, Samus N, Hilchevsky OM. Nitroksylni birradykaly na adamantanoviy platformi . RNAS Ukr 2012; 4: 138–44. [in Ukrainian]
6. Acelajado MC, Hughes ZH, Oparil S, Calhoun DA. Treatment of Resistant and Refractory Hypertension. Circulation Research. 2019; 124: 1061–1070. doi.org/10.1161/CIRCRESAHA.118.312156.
7. Burlaka YB, Sukhoveev OV, Grin NV, Khilchevskyi OM, Verevka SV. EPR spectroscopy studies of changes in erythrocyte membranes in patients with laryngeal cancer. Experimental Oncology. 2017; 39(1): 49–52.
8. Carey RM, Calhoun DA, Bakris GL, Brook RD, Daugherty SL, Dennison-Himmelfarb CR, Egan BM, Flack JM, Gidding SS, Judd E, Lackland DT, Laffer CL, Newton-Cheh C, Smith SM, Taler SJ, Textor SC, Turan TN, White WB. Resistant hypertension: detection, evaluation, and management: a scientific statement from the American Heart Association. Hypertension. 2018. 72(5): 53–90. doi: 10.1161/HYP.0000000000000084.
9. Kotur-Stevuljević J, Vekić J, Stefanović A, Zeljković A, Ninić A, Ivanišević J. Paraoxonase 1 and atherosclerosis-related diseases. Biofactors. 2020; 46(2): 193–205. doi: 10.1002/biof.1549.
10. Krzeminska J, Wronka M, Mlynarska E, Franczyk B, Rysz J. Arterial hypertension – oxidative stress and inflammation. Antioxidants. 2022; 11: 172. doi: 10.3390/antiox11010172.
11. Spilovska K, Zemek F, Korabecny J, Nepovimova E, Soukup O, Windisch M, Kuca K. Adamantane – a lead structure for drugs in clinical practice. Curr Med Chem. 2016; 23(29): 3245–3266. doi: 10.2174/0929867323666160525114026.
12. Szaroma W, Dziubek K. Changes in the amount of reduced glutathione and activity of antioxidant enzymes in chosen mouse organs influenced by zymosan and melatonin administration. Acta Biol. Hung. 2011; 62(2):133–41. doi: 10.1556/ABiol.62.2011.2.3.
13. Tain Y-L, Hsu C-N. Oxidative stress-induced hypertension of developmental origins: preventive aspects of antioxidant therapy. Antioxidants. 2022; 11: 511. doi: 10.3390/antiox11030511.
14. Tzouy RM, Rios FJ, Alves-Lopes R, Neves KB, Camargo LL, Montezano AC. Oxidative stress: a unifying paradigm in hypertension. Canadian Journal of Cardiology. 2020; 36: 659–70. doi: 10.1016/j.cjca.2020.02.081.
15. Vasylchenko VS, Korol LV, Kuchmenko OB, Stepanova NM. The oxidative status in patients with chronic kidney disease. Ukrainian Biochemical Journal. 2020; 92(5): 70–7. doi: 10.15407/ubj92.05.070.

Publication:

«World of Medicine and Biology» Vol. 18 No. 81 (2022) , с. 82-88
УДК 616.12-008.33

DOI:

https://doi.org/10.26724/2079-8334-2022-3-81-82-88