INVESTIGATION OF HUMORAL IMMUNITY INDICATORS OF THE ORAL CAVITY IN PATIENTS DEPENDING ON THE DEGREE OF GENERALIZED CHRONIC PERIODONTITIS

Authors:

Abstract:

The purpose of this research was to investigate the level of oral humoral immunity factors in patients depending on the degree of generalized chronic periodontitis. The study involved 60 patients. The material for the study was unstimulated oral fluid. Determination of lysozyme activity was carried out by the nephelometric method of V.G. Dorofeychuk. The level of sIgA in the oral fluid was identified by enzyme-linked immunosorbent assay according to the manufacturer's instructions. The correlation coefficient (Pearson's r) was computed to determine if a relationship exists between the levels of sIgA and lysozyme with the degree of generalized chronic periodontitis. It has been established an increase in the level of immune factors in the oral fluid of patients with generalized chronic periodontitis of I degree. A progressive significant decrease in lysozyme and sIgA was observed under conditions of generalized chronic periodontitis development of II and III degree. The lowest level of these indices was recorded in patients with generalized chronic periodontitis of III degree. We have established an inverse correlation between the levels of sIgA and lysozyme in the oral fluid of patients with the degree of generalized chronic periodontitis. Thus, the degree of generalized chronic periodontitis affects the level of humoral immune factors in the oral fluid and has an inverse correlation with them.

Keywords:

generalized chronic periodontitis lysozyme humoral immune factors sIgA

References:

1. Vasyleva NA, Bulhakova AY, Ymelbaeva EA, Vasylev EA. Otsenka lokalnoho immuniteta polosti rta pri tradytsyonnoy terapii vospalitelnykh zabolevanyi parodonta. Problemy stomatolohiyi. 2018; 14 (3): 11-16. [in Russian]
2. Hasiuk NV, Yeroshenko HA, Palii OV. Suchasni uyavlennia pro etyolohiiu ta patohenez khvorob parodonta. Svit medytsyny ta biolohii. 2013; 2(38):203-207. [in Ukrainian]
3. Hasiuk NV, Yeroshenko HA. Kharakterystyka klitynnoho skladu yasen pry heneralizovanomu parodontyti. Svit medytsyny ta biolohii. 2015; 1(48):17-20. [in Ukrainian]
4. Nazarchuk OA, Faustova MO. Kliniko-imunolohichne doslidzhennia efektyvnosti zastosuvannia antyseptykiv u likuvanni infektsiino-zapalnykh uskladnen odontoimplantatsii. Biomedical and Biosocial Anthropology. 2017; 28:63-66. [in Ukrainian]
5. Faustova MA, Dobrovolskaya OV, Dobrovolskyi OV. Izmenenie aktivnosti lyzotsyma rotovoy zhidkosti pri dentalnoy implantatsii. Dental science and practice. 2015; 3-4:22-25. [in Russian]
6. Cekici A, Kantarci A, Hasturk H, Van Dyke TE. Inflammatory and immune pathways in the pathogenesis of periodontal disease. Periodontol 2000.2014;64(1):57-80.
7. Fischer RG, Junior RL, Retamal-Valdes B, et al. Periodontal disease and its impact on general health in Latin America. Section V: Treatment of periodontitis. Braz. Oral Res. 2020;34(supp1):e026
8. Hienz SA, Paliwal S, Ivanovski S. Mechanisms of Bone Resorption in Periodontitis. J Immunol Res. 2015;2015:615486.
9. Kassebaum NJ, Bernabe E, Dahiya M, Bhandari B, Murray CJ, Marcenes W. Global burden of severe periodontitis in 1990-2010: a systematic review and meta-regression. J Dent Res. 2014;93(11):1045-1053.
10. Matarese G, Ramaglia L, Fiorillo L, et al. Implantology and Periodontal Disease: The Panacea to Problem Solving? Open Dent J. 2017;11:460–465.
11. Papapanou PN, Sanz M, Buduneli N, Dietrich T, Feres M, Fine DH, et al. Periodontitis: Consensus report of workgroup 2 of the 2017 World Workshop on the Classification of Periodontal and Peri-Implant Diseases and Conditions. J Periodontol. 2018;89(1):173-82.
12. Slots J. Life-threatening pathogens in severeprogressive periodontitis: Focal infection risk, future periodontal practice, role of the Periodontology 2000. Periodontol 2000. 2020; 84(1):215-216.
13. Slots J. Primer on etiology and treatment of progressive/severe periodontitis: A systemic health perspective. Periodontol 2000. 2020;83(1):272-276.

Publication:

«World of Medicine and Biology» Vol. 17 No. 75 (2021) , с. 111-114
УДК 616.314.17-002-071

DOI:

https://doi.org/10.26724/2079-8334-2021-1-75-111-114