CRYOPRESERVED MESENCHYMAL STEM CELLS IMPACT ON THE SPINAL CORD TISSUE IN RATS WITH EXPERIMENTAL ALLERGIC ENCEPHALOMYELITIS

Authors:

Abstract:

Mesenchymal stem cells from human umbilical cord (1×106) are able to correct neurodegenerative changes in the spinal cord of rats with chronic experimental allergic encephalomyelitis under the suboccipital administration. Cells cryopreserved in solution with 3 % dimethyl sulfoxide, 15 % ethylene glycol, 10 % sucrose, 12 % trehalose, 60 % fetal calf serum, slow the build-up of pathological changes at day 35; cryopreserved in solution with 4 % dimethyl sulfoxide, 6 % trehalose, 90 % fetal calf serum, – at days 35–60; cryopreserved in solution with 10 % dimethyl sulfoxide, 90 % fetal calf serum, – have no effect. Selection of optimal cryoprotectants to save the biological properties of these cells and working out the modes and ways of their administration remains relevant.

Keywords:

mesenchymal stem cells human umbilical cord suboccipital administration spinal cord pathologically altered neurons demyelination cryoprotectants

References:

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Publication:

«World of Medicine and Biology» Vol. 17 No. 75 (2021) , с. 215-220
УДК 616.832-002-056.3:591.8:612.014.43:616-092.9

DOI:

https://doi.org/10.26724/2079-8334-2021-1-75-215-220