KLOTHO PROTEIN AND MITOCHONDRIAL SUPEROXIDE DISMUTASE IN YOUNG PERSONS WITH GASTROESOPHAGEAL REFLUX DISEASE AND AUTOIMMUNE THYROIDITIS

Authors:

Abstract:

The purpose of the work was to study the levels of the Klotho protein and mitochondrial superoxide dismutase in young patients with a comorbid course of gastroesophageal reflux disease and autoimmune thyroiditis. Study conducted 165 students: 120 with gastroesophageal reflux disease and autoimmune thyroiditis and 45 with isolated gastroesophageal reflux disease. Klotho protein and mitochondrial superoxide dismutase were studied in blood serum by enzyme immunoassay. The study of mitochondrial superoxide dismutase and Klotho protein showed a significant increase in these indicators in patients with gastroesophageal reflux disease and autoimmune thyroiditis in comparison with the control group and the group with isolated gastroesophageal reflux disease. The Klotho protein and mitochondrial superoxide dismutase can be used as biomarkers of gastroesophageal reflux disease progression in young patients with concomitant autoimmune thyroiditis.

Keywords:

gastroesophageal reflux disease autoimmune thyroiditis Klotho protein mitochondrial superoxide dismutase

References:

1. Lavryshyn YY, Varkholyak IS, Martyschuk TV, Guta ZА, Ivankiv LB, Paladischuk ОR, Murska SD, Gutyj BV, Gufriy DF. Biolohichne znachennya systemy antyoksydantnoho zakhystu organizmu tvaryn. naukovyi visnyk lvivskoho natsionalnoho universytetu veterynarnoyi medytsyny ta biotehnolohiy imeni S.Z. Gzhitskogo. 2016; 18,2(66):100–111. doi:10.15421/nvlvet6622 [in Ukrainian]
2. Simbirtsev AS. Immunofarmakologicheskie aspekty sistemy tsitokinov. Byulleten sibirskoy meditsiny. 2019; 18(1):84-95. doi:10.20538/1682-0363-2019-1-84-95 [in Russian]
3. Antunes C, Curtis SA. Gastroesophageal Reflux Disease. [Updated 2019 Sep 30]. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2020 Jan. Available from: https://www.ncbi.nlm.nih.gov/books/NBK441938/
4. Chen H, Huang X, Fu C et al. Recombinant Klotho Protects Human Periodontal Ligament Stem Cells by Regulating Mitochondrial Function and the Antioxidant System during H2O2 - Induced Oxidative Stress. Oxidative Medicine and Cellular Longevity. 2019; 1-14. doi: 10.1155/2019/9261565.
5. Dunbar KB, Agoston AT, Odze RD, et al. Association of Acute Gastroesophageal Reflux Disease With Esophageal Histologic Changes. JAMA. 2016; 315(19):2104–2112. doi: 10.1001/jama.2016.5657
6. Giri AK, Rawat JK, Singh M, Gautam S, Kaithwas G. Effect of lycopene against gastroesophageal reflux disease in experimental animals. BMC Complement Altern Med. 2015; 15:110. doi: 10.1186/s12906-015-0631-6.
7. Holley AK, Bakthavatchalu V, Velez-Roman JM, St Clair DK. Manganese superoxide dismutase: guardian of the powerhouse. Int J Mol Sci. 2011; 12(10):7114-62. doi: 10.3390/ijms12107114. Epub 2011 Oct 21. PMID: 22072939; PMCID: PMC3211030.
8. Kim JH, Hwang KH, Park KS, Kong ID, Cha SK. Biological Role of Anti-aging Protein Klotho. Journal of lifestyle medicine. 2015; 5(1):1–6. doi: 10.15280/jlm.2015.5.1.1
9. Lim SW, Jin L, Luo K. Klotho enhances FoxO3-mediated manganese superoxide dismutase expression by negatively regulating PI3K/AKT pathway during tacrolimus-induced oxidative stress. Cell Death Dis. 2017; 8(8): e2972. doi:10.1038/cddis.2017.365.
10. Mancini A, Di Segni C, Raimondo S et al. Thyroid Hormones, Oxidative Stress, and Inflammation. Mediators of inflammation. 2016; 6757154. doi: 10.1155/2016/6757154
11. Martinucci I, Natilli M, Lorenzoni V. Gastroesophageal reflux symptoms among Italian university students: epidemiology and dietary correlates using automatically recorded transactions. BMC Gastroenterol. 2018; 18:116. doi: 10.1186/s12876-018-0832-9
12. Mudyanadzo TA. Barrett's Esophagus: A Molecular Overview. Cureus. 2018; 10(10):e3468. Published 2018 Oct 19. doi:10.7759/cureus.3468
13. Olejnik A, Franczak A, Krzywonos-Zawadzka A, Kałużna-Oleksy M, Bil-Lula I. The Biological Role of Klotho Protein in the Development of Cardiovascular Diseases. Biomed Res Int. 2018; 2018:5171945. Published 2018 Dec 24. doi:10.1155/2018/5171945
14. Yanaka A. Role of NRF2 in protection of the gastrointestinal tract against oxidative stress. Journal of clinical biochemistry and nutrition. 2018; 63(1): 18–25. doi: 10.3164/jcbn.17-139
15. Yao Y, Wang Y, Zhang Y. Klotho ameliorates oxidized low density lipoprotein (ox-LDL)-induced oxidative stress via regulating LOX-1 and PI3K/Akt/eNOS pathways. Lipids Health Dis. 2017; 16:77. doi: 10.1186/s12944-017-0447-0

Publication:

«World of Medicine and Biology» Vol. 16 No. 74 (2020) , с. 49-53
УДК [616.333-008.6:616.441-002]-053.67-078:57.083.3

DOI:

https://doi.org/10.26724/2079-8334-2020-4-74-49-53