GENETIC PREDISPOSITION TO BRONCHOPULMONARY PATHOLOGY

Authors:

Abstract:

We studied 215 people who work in harmful industries, 90 of which had a history of bronchopulmonary pathology of occupational etiology and 125 of them without such pathology. The following polymorphisms of DNA repair genes were identified in real-time using polymerase chain reaction: XPD (rs13181, rs799793), ERCC1 (rs11615), XRCC3 (rs861539), XRCC1 (rs25487), ATM (rs664677), XRCC7 (rs7003908) and MLH1 (rs1799977). We studied the frequency distribution of the genotypes of DNA repair genes with the subsequent integral statistical analysis of the data obtained. Analysis of the results made it possible to build a mathematic model that included two single nucleotide polymorphisms: XRCC1 (rs25487) and ATM (rs664677), which in this study represented the two main independent effects with the greatest predictive power 80.35 % for the results of binary logistic regression and of the method of multivariate dimension reduction.

Keywords:

SNP XPD ERCC1 XRCC3 XRCC1 АТМ XRCC7 MLH1 bronchopulmonary pathology

References:

1. Andrushchenko ТА, GoncharovSV, DosenkoVE.Genetichnashilnistdobroncholegenevoipatologiiupracivnikivshidlivihinebezpechnihgaluzeypromislovosti: аnalizpyatipolymorphismoivgenovreparaciiDNK. FiziologichnyiZhurnal. 2018; 64 (4): 12-19. doi: 10.15407/fz64.04.012. [inUkrainian].
2. IzmerovNFProfesionalniezabolevaniyaorganovdihaniya (Nacionalnoerukovodstvo). Моskva: izdatelskayagrupa «GEOTAR-МEDIA»; 2015: 785: 119-148. [inRussian].
3. UrzhumovPV, PogodinaAV, AkleevAVPolimirphismigenov NBS1iPARP1ieffectivnoctreparaciiDNK. VestnikChelyabinskogogosudarstvennogo universiteta. №7 (298). Biologia. Vip.2: 107-108. [in Russian].
4. Caporaso N, Pickle LW, Bale S, Ayesh R, Hetzel M, Jefferey I, Rao DC The distribution of debrisoquine metabolic phenotypes and implications for the suggested association with lung cancer risk. Genetic epidemiology. 1989; 6: 517-524.
5. Gong Yang, Xiao-Ou Shu, Zhi-Xian Ruan BS, Qiu-Yin Cou, Fan Jin, Yu-Tang Gao, Wei Zheng Genetic polymorphisms in glutation-S- transferase genes (GSTM1; GSTT1; GSTP1) and survival after chemotherapy for invasive breast carcinoma. Cancer. 2005; 103(1): 52-58.
6. Hao B, Miao X, Li Y, Zhang X, Sun T, et al. A novel T–77C polymorphism in DNA repair gene XRCC1 contributes to diminished promoter activity and increased risk of non-small cell lung cancer. Oncogene, 25. 2006: 3613–3620.
7. Hsieh YH, Chang WS, Tsai CW et al. DNA double-strand break repair gene XRCC7 genotypes were associated with hepatocellular carcinoma risk in Taiwanese males and alcohol drinkers, Tumour Biol. 2015. 36(6): 4101-4106.
8. Kiffmeyer WR, Langer E, Davies SM Genetic Polymorphismsin the HumPopul.Cancer.2004; 100. (2): 411-417.
9. Kuschel B, Auranen A, McBride S, Novik KL, Antoniou A Variants in double-strand break repair genes and breast cancer susceptibility. Hum Mol Genet 2002. (11): 1399-1440.
10. Lanza G, Gafa R, Maestri I et al. Immunohistochemical pattern of MLH1/MSH2 expression is related to clinical and pathological features in colorectal adenocarcinomas with microsatellite instability. Mol Pathol. 2002. 15(7): 741-749.
11. Rodriguez S, Gaunt TR, Day NM Hardy-Weinberg Equilibrium Testing ofAscertainment for Mendelian Randomization Studies.American J BiolEpidemiol. 2009; 10: 1093-1099.
12. Tretyak B, Makukh H, Kitsera N et al. The molecular genetic analysis of common ATM gene mutations among patients with Ataxia-telagiectasiasuspection, Factors of experimental evolution of organisms. 2015. vol. 16: 251-255.
13. Vineis P, Hoek G, Krzyzanowski M, Vigna-Taglianti F, Veglia F, Airddi L Lung cancer attributable to environmental tobacco smoke and air pollution in non-smokers in different European countries: a prospective study. Environ Health. 2007; doi:10.1186/1476-069x-6-7.
14. Wang Y, Yang H, Li H, Li L, Wang H et al. Association between X-ray repair cross complementing group 1 codon 399 and 194 polymorphisms and lung cancer risk: a meta-analysis. Cancer. 2009; 285: 134–140.
15. Xiao M, Shen Y, Chen L et al. The rs7003908 (T>G) polymprphism in the XRCC7 gene and the risk of cancers, Mol. Biol. Rep. 2014. 41(6): 3577-3582. 616.23/24-057:575.113.1

Publication:

«World of Medicine and Biology» Vol. 15 No. 68 (2019) , с. 7-11
УДК 616.23/24-057:575.113.1

DOI:

https://doi.org/10.26724/2079-8334-2019-2-68-7-11