COMBINED EFFECT OF GLYPHOSATE, SACCHARIN AND SODIUM BENZOATE ON THE GUT MICROBIOTA IN RATS
Experemental medicine

COMBINED EFFECT OF GLYPHOSATE, SACCHARIN AND SODIUM BENZOATE ON THE GUT MICROBIOTA IN RATS

Published 2019-05-15

Authors:

M.V. Bilan
V.V. Brygadyrenko
M.A. Lieshchova
N.M. Tishkina

Abstract:
In a laboratory experiment on adult white rats, it has been determined that addition of glyphosate, as well as glyphosate in a mixture with saccharin, to the diet contributes to broader reproduction of microorganisms of the Klebsiella, Citrobacter, Enterobacter and Pseudomonas genera. Mixtures of glyphosate and food additives allow conditionally pathogenic yeast-like Candida fungi (Candida glabrata and C. albicans) to spread more widely in the intestine. Significant fluctuations in the number of Enterococcus spp. bacteria genus were observed: by 108 times within range of each of the three experimental groups of rats with addition of herbicide with benzoate and saccharin to the diet.
Keywords:
gut microbiota glyphosate herbicides saccharin benzoate
References:
  1. Anderson RL, Kirkland JJ. The effect of sodium saccharin in the diet on caecal microflora. Food and Cosmetics Toxicology. 1980; 18(4):353–5. doi:10.1016/0015-6264(80)90188-1
  2. Behrens M, Blank K, Meyerhof W. Blends of non-caloric sweeteners saccharin and cyclamate show reduced off-taste due to TAS2R bitter receptor inhibition. Cell Chemical Biology. 2017; 24(10):1199–204.e2. doi:10.1016/j.chembiol.2017.08.004
  3. Bian X, Tu P, Chi L, Gao B, Ru H, Lu K. Saccharin induced liver inflammation in mice by altering the gut microbiota and its metabolic functions. Food and Chemical Toxicology. 2017;107:530–9. doi:10.1016/j.fct.2017.04.045
  4. Boyko OO, Brygadyrenko VV. The impact of certain flavourings and preservatives on the survivability of larvae of nematodes of Ruminantia. Regulatory Mechanisms in Biosystems. 2018; 9(1):118–23. doi:10.15421/021817
  5. Boyko AA, Brygadyrenko VV. Changes in the viability of Strongyloides ransomi larvae (Nematoda, Rhabditida) under the influence of synthetic flavourings. Regulatory Mechanisms in Biosystems. 2017;8(1):36–40. doi:10.15421/021707
  6. Daly K, Darby AC, Hall N, Wilkinson MC, Pongchaikul P, Bravo D, Shirazi-Beechey SP. Bacterial sensing underlies artificial sweetener-induced growth of gut Lactobacillus. Environmental Microbiology. 2015;18(7):2159–71. doi:10.1111/1462-2920.12942
  7. Labrecque MT, Malone D, Caldwell KE, Allan AM. Impact of ethanol and saccharin on fecal microbiome in pregnant and non-pregnant mice. Journal of Pregnancy and Child Health. 2015;2:5. doi:10.4172/2376-127x.1000193
  8. Lieshchova MA, TishkinaNM, Bohomaz AA, Gavrilin PM, Brygadyrenko VV. Combined effect of glyphosphate, saccharin and sodium benzoate on rats. Regulatory Mechanisms in Biosystems. 2018;9(4):591–7. doi:10.15421/021888
  9. Lieshchova MA, Brygadyrenko VV, Tishkina NM, Gavrilin PM, Bohomaz AA. Impact of polyvinyl chloride, polystyrene, and polyethylene on the organism of mice. Regulatory Mechanisms in Biosystems. 2019;10(1):50–55. doi:10.15421/021908
  10. Lozano VL, Defarge N, Rocque LM, Mesnage R, Hennequin D, Cassier R, et al. Sex-dependent impact of Roundup on the rat gut microbiome. Toxicology Reports. 2018;5:96–107. doi:10.1016/j.toxrep.2017.12.005
  11. Mao Q, Manservisi F, Panzacchi S, Mandrioli D, Menghetti I, Vornoli A, et al. The Ramazzini Institute 13-week pilot study on glyphosate and Roundup administered at human-equivalent dose to Sprague Dawley rats: Effects on the microbiome. Environmental Health. 2018;17:50. doi:10.1186/s12940-018-0394-x
  12. Martynov VO, Brygadyrenko VV. The influence of synthetic food additives and surfactants on the body weight of larvae of Tenebrio molitor (Coleoptera, Tenebrionidae). Biosystems Diversity. 2017; 25(3):236–42. doi:10.15421/011736
  13. Naim M, Zechman JM, Brand JG, Kare MR, Sandovsky V. Effects of sodium saccharin on the activity of trypsin, chymotrypsin, and amylase and upon bacteria in small intestinal contents of rats. Experimental Biology and Medicine. 1985;178(3):392–401. doi:10.3181/00379727-178-42022
  14. Putnikov AV, Holota YV, Sergeychuk TM, Ostapchuk AM, Zakordonets LV, Ostapchenko LI, Tolstanova GM. Quantitative and functional characteristics of rat intestinal microbiota. Microbiology and Biotechnology. 2015;2:89–100.
  15. Suez J, Korem T, Zeevi D, Zilberman-Schapira G, Thaiss CA, Maza O, et al. Artificial sweeteners induce glucose intolerance by altering the gut microbiota. Nature. 2014;514:181–6. doi:10.1038/nature13793
Publication:
«World of Medicine and Biology» Vol. 15 No. 68 (2019) , с. 171-175
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