INFLUENCE OF IMIDAZOLINES MIXTURES ON THE ACTIVITY OF NADPH-CYTOCHROME C REDUCTASE AND CYTOCHROME P-450 IN A MICROSOMAL FRACTION OF RAT LIVER

Authors:

Abstract:

The activity of enzymes in a microsomal fraction of rat liver on the 30th day of the effects of industrial chemical pollutants - imidazoline mixtures at doses of 1/10 and 1/100 DL50 was determined with the help of spectrophotometric method, which was necessary for the complete explanation of the biochemical mechanisms of their membrane-tropic action. Imidazoline-containing mixtures with alkyl radicals C7-9 and C9-15 at a dose of 1/10 DL50 cause inactivation of microsomal NADPH reductase system, proven by reduced activity of NADPH-cytochrome c reductase and decrease in cytochrome P-450 level. On the contrary, prolonged exposure of mixtures at a dose of 1/100 DL50 leads to its activation, as evidenced by increased activity of NADPH-cytochrome c reductase and level of cytochrome P-450. The revealed changes can be considered as one of the pathogenetic links of biochemical mechanisms of the membrane-tropic action of imidazoline mixtures that need to be taken into account in the development of methods for their correction.

Keywords:

mixtures of imidazolines rats microsomes NADPH reductase cytochrome P-450

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Publication:

«World of Medicine and Biology» Vol. 12 No. 56 (2016) , с. 129-132
УДК 577.152.1:616.36-092.9-099:543.395