PROLIFERATION AND ULTRASTRUCTURE OF PERIVASCULAR CELLS IN BONE TISSUE REMODELING ZONES AT REMOVAL SUPPORT LOADING

Authors:

Abstract:

Using histological methods, electron microscopy and histoautography with 3H-thymidine was found that at long term hind limbs unloading the poorly differentiated perivascular cells of sinusoids are the main DNA- synthesizing fraction in the zones of adaptive transformations (metaphysis, cortical bone vascular channels in diaphysis) in rat femur. High proliferative activity of the population is connected not only to self-maintenance but also takes part in genesis of osteogenic cells. It was found, that under supportive unloading there is reduction of perivascular cells proliferation intensity in destruction zones, particularly in differentiating cells population, which includes preosteoblasts in remodeling zones. Part of the cells differentiates into fibroblasts, resulting in bone fibrosis zones emerge. Thus, there isn’t an adequate response of the bone tissues in destruction locus. The osteoporotic cavities are forming in bone tissue; mechanical density of bone structures is reducing.

Keywords:

perivascular cells proliferation ultrastructure

References:

1. Brusko A.T. Funkcional'naja perestrojka kostej i ee klinicheskoe znachenie: Monogr. / A.T. Brusko, G.V. Gajko // – L.: LGMU, -2005. – 212 c.
2. Buravkova L. B. Chuvstvitel'nost' stromal'nyh kletok-pedshestvennikov razlichnoj kommitirovannosti k modelirovannoj mikrogravitacii // Doklady Akademii Nauk. – 2010. – №2. – S. 267–271.
3. Oganov V. S. O mehanizmah osteopenii i osobennostjah metabolizma kostnoj tkani cheloveka v uslovijah nevesomosti / V.S. Oganov, A.I. Grigr'ev // Rossijskij fiziologicheskij zhurnal im. I.M. Sechenova. – 2012. – № 3. – S. 395-409.
4. Oganov. V. S. Kostnaja sistema, nevesomost' i osteoporoz. Izd. 2-e, pererab. i dop. / V.S. Oganov // – V.: Nauchnaja kniga, - 2014. - 291 s.
5. Rodionova N.V. Funkcional'naja morfologija kletok v osteogneze / N.V. Rodionova.-Kiev: Nauk.dumka,-1989,186 s.
6. Rodіonova N. V. Citologіchnі mehanіzmi perebudov u kіstkah pri gіpokіnezії ta mіkrogravіtacії / N.V. Rodіonova // – Kiїv, Naukova dumka, - 2006. – 238 s.
7. Da Silva Meirelles L. Mesenchymal stem cells reside in virtually all post-natal organs and tissues / L. Da Silva Meirelles, P.C. Chagastelles, N. B. Nardi // Journal of Cell Science. – 2006. – Vol. 119 (11). - Р. 2204-2213.
8. Crisan M. A perivascular origin for mesenchymal stem cells in multiple human organs / M. Crisan, S. Yap, L. Casteilla [et all.] // Cell stem cell. – 2008. – Vol.3. – P. 301-313
9. Rodionova N. V. Differentiation potentials of perivascular cells in the bone tissue remodeling zones under microgravity (40th COSPAR Scientific Assembly, 2014) / N.V. Rodionova, O.V. Katkova // Moscow. – 2014. – F 5.2-2014-14 с.
10. Rodionova N.V. Ultrastructure and differentiation of perivascular cells in the bone tissue remodeling zones under microgravity / N.V. Rodionova, O.V. Katkova // 14-а Українська конференція з космічних досліджень, 8-12 вер. 2014. –Ужгород, - 2014. – 40 с.

Publication:

«World of Medicine and Biology» Vol. 11 No. 49 (2015) , с. 120-123
УДК 576.536+611.71/72