MORPHOLOGICAL AND FUNCTIONAL STATE OF THE MACROPHAGAL LINK OF THE SPLEEN UNDER CONDITIONS OF OXIDATIVE-NITROSATIVE STRESS CAUSED BY PROLONGED ADMINISTRATION OF A COMPLEX OF CHEMICAL FOOD ADDITIVES

Authors:

Abstract:

The study investigates the effect of long-term administration of a dietary supplement complex on the state of the macrophage link in the spleen of rats. The experiment was conducted on 70 male rats that received a supplement mixture for 1–20 weeks. The levels of malondialdehyde, nitrites, and the immunomorphology of CD68+ macrophages were assessed. A biphasic reaction of the macrophage population was established. In the early stages, an increase in cell number was observed, peaking at week 4, at 1.79 times the control. Chronic exposure led to a marked reduction in cell numbers by week 20, with cell numbers 4.48 times lower than the initial values. Morphological analysis revealed progressive cell fragmentation, atrophy of the marginal zone, and decreased immunohistochemical staining intensity. Structural degradation of the macrophage apparatus closely correlated with the accumulation of lipid peroxidation products. The study demonstrates the mechanism of depletion of the spleen's immune barrier under prolonged xenobiotic stress.

Keywords:

immune system morphological changes oxidative stress nitrosative stress spleen white pulp red pulp food additives CD68 malondialdehyde monosodium glutamate sodium nitrite ponceau 4R

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Publication:

«World of Medicine and Biology» Vol. 22 No. 1 (2026) , с. 199-202
УДК 616.411-03:612.017:612.015-02:613.29

DOI:

https://doi.org/10.26724/2079-8334-2026-1-95-199-202