EFFECT OF CARBON DIOXIDE HOP EXTRACT ON GROWTH INTENSITY AND ANTIBIOTIC RESISTANCE LEVEL OF THE TRANSFORMANT STRAIN KLEBSIELLA PNEUMONIAE

Authors:

Abstract:

The effect of hop carbon dioxide extract on the growth and ampicillin resistance of the museum strain Klebsiella pneumoniae 5055 with an experimentally acquired beta-lactam antibiotic resistance plasmid from Escherichia coli "D"strain has been studied. The transfer of the antibiotic resistance plasmid to the specified strain has been confirmed by the method of horizontal gel electrophoresis, real-time polymerase chain reaction and when studying the antibiotic sensitivity profile by phenotypic methods. It was found that hop carbon dioxide extract produced a pronounced antimicrobial effect on the transformant strain of Klebsiella pneumoniae, but did not affect the level of its resistance to ampicillin. After analyzing the data of our studies, it can be assumed that the inhibitory effect of the carbonic acid hop extract on the growth of bacteria was not accompanied by an antiplasmid effect, since resistance to beta-lactams in the Klebsiella pneumoniae 5055+pECD strain was associated precisely with the acquisition of an ampicillin resistance plasmid by in vitro transformation.

Keywords:

carbonic acid hop extract transformant strain of Klebsiella pneumoniae antimicrobial activity antiplasmid effect

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Publication:

«World of Medicine and Biology» Vol. 22 No. 96 (2026) , с. 171-174
УДК 579.61 + 615.28:615.32:615.45

DOI:

https://doi.org/10.26724/2079-8334-2026-2-96-171-174