MORPHOLOGICAL CHANGES IN THE STRUCTURAL ELEMENTS OF THE EYEBALL IN CONTUSION INJURY IN RABBITS

Authors:

Abstract:

The purpose of the study was to investigate pathomorphological, morphometric, and densitometric changes in the structural elements of the eyeball (choroid and retinal neurons) in rabbits during modeled contusion syndrome, and to evaluate the comparative efficacy of l-lysine-(s)-2,6-diaminohexanoic acid 3-methyl-1,2,4-triazolyl-5-thioacetate 1 % and citicoline 2 % eye drops with hyaluronic acid. The study was performed on 20 California rabbits with modeled ocular contusion injury. Animals were treated with either l-lysine-(s)-2,6-diaminohexanoic acid 3-methyl-1,2,4-triazolyl-5-thioacetate 1 % or citicoline 2 % eye drops with hyaluronic acid 3 times a day. After 10 days, retinal sections were analyzed morphometrically and densitometrically using the VIDAS-386 system to evaluate nuclear area, RNA concentration, cell density, percentage of apoptotic neurons. Untreated ocular contusion injury led to pronounced retinal neurodegeneration, manifested by a significant decrease in neuronal density, nuclear area, RNA concentration, and thickness of all retinal layers, alongside a sharp increase in apoptosis. Both investigated drugs exerted a distinct neuroretinoprotective effect, stimulating transcriptional processes and decreasing apoptotic cell death. Notably, l-lysine- (s)-2,6-diaminohexanoic acid 3-methyl-1,2,4-triazolyl-5-thioacetate demonstrated superior therapeutic efficacy over citicoline 2 % eye drops with hyaluronic acid, showing a statistically significant advantage in increasing retinal layers thickness, density of ganglion cells, bipolar cells. Course administration of l-lysine-(s)-2,6-diaminohexanoic acid 3-methyl-1,2,4-triazolyl-5-thioacetate 1 % eye drops shows high neuroretinoprotective potential, exceeding the reference drug citicoline 2 % eye drops with hyaluronic acid under conditions of experimental ocular trauma.

Keywords:

contusion syndrome retina choroid experimental ocular contusion neuroprotection apoptosis

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Publication:

«World of Medicine and Biology» Vol. 22 No. 96 (2026) , с. 188-193
УДК 617.7-001.34-091.8-092.9

DOI:

https://doi.org/10.26724/2079-8334-2026-2-96-188-193