ASSESSMENT OF THE EFFECT OF THERAPEUTIC AND PREVENTIVE MEASURES ON THE ANTIMICROBIAL FACTOR OF THE ORAL CAVITY IN THE ORAL FLUID OF PATIENTS AFTER SURGICAL INTERVENTION TO REMOVE TUMORS AND CHEMOTHERAPY

Authors:

Abstract:

The study was devoted to the evaluation of the effect of a therapeutic complex of drugs on the indicator of the main antimicrobial factor of the oral cavity – lysozyme activity in the oral fluid of patients after surgical removal of tumors and chemotherapy. Thirty-five adults (25–55 years) were enrolled: a healthy control cohort, a comparison group receiving only standard oncologic care, and a main group receiving standard care plus the therapeutic and preventive complex. Non-stimulated whole saliva was collected at baseline and at 1-, 3-, 6-, and 12-month post-surgery. Lysozyme activity was quantified spectrophotometrically. Results were analysed with Student’s t-test (p<0.01). Findings demonstrate that adjunctive use of the therapeutic and preventive complex effectively reconstitutes the salivary antimicrobial barrier compromised by surgery and chemotherapy, supporting its clinical utility for preventing infection-related sequelae in this high-risk population.

Keywords:

head-and-neck tumors lysozyme saliva lipid peroxidation dentistry therapeutic-preventive complex

References:

1. Makarenko OA, Khromahina LM, Khodakov IV. Metody doslidzhennya stanu kyshkovyka ta kistok u laboratornykh shchuriv: dovidnyk. Odesa: Odeskyy natsionalnyy universytet im. I.I. Mechnykova, 2022. 81 p. [in Ukrainian].
2. Rohach IM, Keretsman AO, Sitkar AD. Pravylno vybranyy metod statystychnoho analizu – shlyakh do yakisnoyi interpretatsiyi danykh medychnykh doslidzhen. Naukovyy visnyk Uzhhorodskoho universytetu, seriya “Medytsyna”. 2017;2(56):124–128. [in Ukrainian].
3. Almhöjd U, Fisic A, Cevik-Aras H, Tuomi L, Finizia C, Almståhl A. Explorative study of stimulated saliva proteome in head and neck cancer patients pre- and post-treatment. Heliyon. 2024 Oct 9;10(20):e39033. DOI: 10.1016/j.heliyon.2024.e39033.
4. Dipalma G, Inchingolo AM, Latini G, Ferrante L, Nardelli P, Malcangi G, et al. The Effectiveness of Curcumin in Treating Oral Mucositis Related to Radiation and Chemotherapy: A Systematic Review. Antioxidants (Basel). 2024 Sep 25;13(10):1160. DOI: 10.3390/antiox13101160.
5. Ferraboschi P, Ciceri S, Grisenti P. Applications of Lysozyme, an Innate Immune Defense Factor, as an Alternative Antibiotic. Antibiotics (Basel). 2021 Dec 14;10(12):1534. DOI: 10.3390/antibiotics10121534.
6. Hong BY, Sobue T, Choquette L, Dupuy AK, Thompson A, Burleson JA, et al. Chemotherapy-induced oral mucositis is associated with detrimental bacterial dysbiosis. Microbiome. 2019 Apr 25;7(1):66. DOI: 10.1186/s40168-019-0679-5
7. Horhat RM, Alexandru A, Ivan CS, Varga NI, Suba MI, Ciurariu E, et al. Nutritional and Supplemental Interventions for Prevention and Treatment of Oral Mucositis in Pediatric Oncology. Nutrients. 2025 Nov 11;17(22):3521. DOI: 10.3390/nu17223521.
8. Moutsopoulos NM, Konkel JE. Tissue-Specific Immunity at the Oral Mucosal Barrier. Trends Immunol. 2018 Apr;39(4):276–287. DOI: 10.1016/j.it.2017.08.005.
9. Peng TR, Tsai FP, Wu TW. Effects of various treatments for preventing oral mucositis in cancer patients: A network meta-analysis. PLoS One. 2022 Dec 8;17(12):e0278102. DOI: 10.1371/journal.pone.0278102.
10. Schuurhuis JM, Span LF, Stokman MA, van Winkelhoff AJ, Vissink A, Spijkervet FK. Effect of leaving chronic oral foci untreated on infectious complications during intensive chemotherapy. Br J Cancer. 2016 Apr 26;114(9):972–8. DOI: 10.1038/bjc.2016.60.
11. Şenel S. An Overview of Physical, Microbiological and Immune Barriers of Oral Mucosa. Int J Mol Sci. 2021 Jul 22;22(15):7821. DOI: 10.3390/ijms22157821.
12. Yang B, Li W, Shi J. Preventive effect of probiotics on oral mucositis induced by anticancer therapy: a systematic review and meta-analysis of randomized controlled trials. BMC Oral Health. 2024 Sep 29;24(1):1159. DOI: 10.1186/s12903-024-04955-7.

Publication:

«World of Medicine and Biology» Vol. 21 No. 94 (2025) , с. 41-45
УДК [616-053.88+616-006.04]:616.31-08-039.71

DOI:

https://doi.org/10.26724/2079-8334-2025-4-94-41-45