ANTIOXIDANT-PROOXIDANT SYSTEM OF THE ORAL MUCOSA IN RATS UNDER CHRONIC STRESS AGAINST THE BACKGROUND OF A THERAPEUTIC-PREVENTIVE COMPLEX IN THE CONTEXT OF PEDIATRIC DENTISTRY

Authors:

Abstract:

The study was devoted to evaluating the impact of a multi-component therapeutic-prophylactic complex on indices of oxidative damage and antioxidant protection in oral mucosal homogenates of rats subjected to chronic acoustic stress. Thirty-four male Wistar rats were randomly assigned to an intact control, a chronic-stress group, and a chronic-stress+therapeutic-prophylactic complex group. Acoustic stress was induced for 6 h per day¹ over 50 days using an LS-912 ultrasonic pest repeller (Leaven Enterprise, Taiwan; 30–60 kHz, 130 dB). Animals in the third group received the therapeutic-prophylactic complex daily throughout the stress period. Catalase activity and malondialdehyde content were assayed in oral-mucosal homogenates, and an antioxidant-prooxidant index was calculated. Findings substantiate further exploration of the therapeutic-prophylactic complex as a promising strategy for preventing oxidative-stress-driven complications of the oral mucosa, particularly in paediatric dentistry.

Keywords:

stress oral mucosa therapeutic-prophylactic complex paediatric dentistry rats experimental study

References:

1. Hubskyy YuI, Nizhenkovska IV, Korda MM. Biolohichna i bioorhanichna khimiya: u 2 kn.: pidruchnyk. Kn.2. Biolohichna khimiya. Kyiv: VSV “Medytsyna”, 2016. 497–506. [in Ukrainian].
2. Nakaz Ukrayiny “Pro zatverdzhennya Poryadku provedennya naukovymy ustanovamy doslidiv, eksperymentiv na tvarynakh”. Ministerstvo osvity i nauky Ukrayiny. 2012;249. [in Ukrainian].
3. Rohach IM, Keretsman AO, Sitkar AD. Pravylno vybranyy metod statystychnoho analizu – shlyakh do yakisnoyi interpretatsiyi danykh medychnykh doslidzhen. Naukovyy visnyk Uzhhorodskoho universytetu, seriya “Medytsyna”. 2017;2(56):124–128. [in Ukrainian].
4. Bao J, Chen C, Yan J, Wen Y, Bian J, Xu M, et al. Antioxidant therapy for patients with oral lichen planus: A systematic review and meta-analysis. Front Pharmacol. 2022 Nov 10;13:1030893. DOI: 10.3389/fphar.2022.1030893.
5. Dipalma G, Inchingolo AM, Latini G, Ferrante L, Nardelli P, Malcangi G, et al. The Effectiveness of Curcumin in Treating Oral Mucositis Related to Radiation and Chemotherapy: A Systematic Review. Antioxidants (Basel). 2024 Sep 25;13(10):1160. DOI: 10.3390/antiox13101160.
6. Juszczyk G, Mikulska J, Kasperek K, Pietrzak D, Mrozek W, Herbet M. Chronic Stress and Oxidative Stress as Common Factors of the Pathogenesis of Depression and Alzheimer's Disease: The Role of Antioxidants in Prevention and Treatment. Antioxidants (Basel). 2021 Sep 9;10(9):1439. DOI: 10.3390/antiox10091439.
7. Li Q, Zhao Y, Deng D, Yang J, Chen Y, Liu J, et al. Aggravating Effects of Psychological Stress on Ligature-Induced Periodontitis via the Involvement of Local Oxidative Damage and NF-κB Activation. Mediators Inflamm. 2022 Feb 16;2022:6447056. DOI: 10.1155/2022/6447056.
8. Maciejczyk M, Zalewska A, Ładny JR. Salivary Antioxidant Barrier, Redox Status, and Oxidative Damage to Proteins and Lipids in Healthy Children, Adults, and the Elderly. Oxid Med Cell Longev. 2019 Dec 5;2019:4393460. DOI: 10.1155/2019/4393460.
9. Macrì M, D'Albis G, D'Albis V, Antonacci A, Abbinante A, Stefanelli R, et al. Periodontal Health and Its Relationship with Psychological Stress: A Cross-Sectional Study. J Clin Med. 2024 May 16;13(10):2942. DOI: 10.3390/jcm13102942.
10. Sardaro N, Della Vella F, Incalza MA, DI Stasio D, Lucchese A, Contaldo M, et al. Oxidative Stress and Oral Mucosal Diseases: An Overview. In Vivo. 2019 Mar-Apr;33(2):289–296. DOI: 10.21873/invivo.11474.
11. Wakamori S, Taguchi K, Nakayama Y, Ohkoshi A, Sporn MB, Ogawa T, et al. Nrf2 protects against radiation-induced oral mucositis via antioxidation and keratin layer thickening. Free Radic Biol Med. 2022 Aug 1;188:206–220. DOI: 10.1016/j.freeradbiomed.2022.06.239.
12. Yin Q, Liu C, Bao H, Li S, Huang Z, Gu D, et al. Estimation of gingival crevicular fluid oxidative stress markers in school-aged children and teenagers with insufficient sleep. BMC Oral Health. 2022 Dec 18;22(1):616. DOI: 10.1186/s12903-022-02642-z.

Publication:

«World of Medicine and Biology» Vol. 21 No. 94 (2025) , с. 211-215
УДК [57.084.1+611.018.4]:616-08-039.71

DOI:

https://doi.org/10.26724/2079-8334-2025-4-94-211-215