EXTRACELLULAR VESICLES OF STEM CELLS AS DIAGNOSTIC BIOMARKERS IN LIQUID BIOPSY OF SOMATIC AND ONCOLOGICAL DISEASES

Authors:

Abstract:

The purpose of the study was to investigate the prognostic value of extracellular vesicles (CD63 expression) in oncological and somatic diseases by liquid biopsies. To test our hypothesis, this case-control study consisted of the indicators of CD63 expression in the blood plasma of 20 healthy donors (control group); 18 patients with a positive diagnosis of COVID-19 according to PCR analysis; 33 patients with critical limb ischemia; 38 patients with renal cell carcinoma; 11 patients with uterine sarcoma. Extracellular vesicle was isolation from human plasma samples. Our studies have shown that the CD63 expression correlates with the progression of the deseases and lower survival. Thus, we performed this analysis to better understand the prognostic value of CD63 expression in somatic and oncologic diseases. Levels of circulating extracellular vesicles (CD63 expression) showed healthy donors group has the lowest mean value; uterine sarcoma group shows the highest mean value; groups with COVID-19 and renal cell cancer demonstrate similar mean values; group with critical limb ischemia shows an intermediate value. The results suggest that the CD63 can be a universal valuable marker in predicting complications and survival in somatic and oncological diseases.

Keywords:

extracellular vesicles. renal cell carcinoma uterine sarcoma COVID-19 critical limb ischemia

References:

1. Dagher J, Delahunt B, Rioux-Leclercq N, Egevad L, Srigley JR, Coughlin G, et al. Clear cell renal cell carcinoma: validation of World Health Organization/International Society of Urological Pathology grading. Histopathology. 2017 Dec;71(6):918-925. doi: 10.1111/his.13311.
2. Deng Y, Cai S, Shen J, Peng H. Tetraspanins: Novel Molecular Regulators of Gastric Cancer. Front Oncol. 2021;11:702510. https://doi.org/10.3389/fonc.2021.702510.
3. Galardi A, Fogazzi V, Tottone C, Giussani M, Pupa SM, Cosentino G, et al. Small extracellular vesicles: messengers at the service of breast cancer agenda in the primary and distant microenvironments. J Exp Clin Cancer Res. 2025;44(1):216. https://doi.org/10.1186/s13046-025-03471.
4. Hosh M, Antar S, Nazzal A, Warda M, Gibreel A, Refky B. Uterine Sarcoma Analysis of 13,089 Cases Based on Surveillance, Epidemiology, and End Results Database. Intern.J.Gynecological cancer. 2016; 26(6): P1098-1104. DOI: 10.1097/IGC.0000000000000720.
5. ICD-0–3. SEER Site/Histology Validation December 5 2012:301-496. Available at http://seer.cancer.gov/icd-o-3/.
6. Jeppesen DK, Fenix AM, Franklin JL, Higginbotham JN, Zhang Q, Zimmerman LJ, et al. Reassessment of Exosome Composition. Cell. 2019;177(2):428-445.e18. https://doi.org/10.1016/j.cell.2019.02.029.
7. Justo BL, Jasiulionis MG. Characteristics of TIMP1, CD63, and β1-Integrin and the Functional Impact of Their Interaction in Cancer. Int J Mol Sci. 2021;22(17):9319. https://doi.org/10.3390/ijms22179319.
8. Kaprio T, Hagstrom J, Andersson LC, Haglund C. Tetraspanin CD63 independently predicts poor prognosis in colorectal cancer. Histol Histopathol. 2020;19:18209. doi: 10.14670/HH-18-209.
9. Koh H, An H, Jung J, Song D. The prognostic significance of CD63 expressionin patients with non-small cell lung cancer. Pol J Pathol. 2019;70:183–188. doi: 10.5114/pjp.2019.90394.
10. Koh HM, Jang BG, Kim DC. Prognostic Value of CD63 Expression in Solid Tumors: A Meta-analysis of the Literature. In Vivo. 2020;34(5):2209-2215. https://doi.org/10.21873/invivo.12031.
11. Komarevtsev VM, Balabanova KV, Chernykh YuA, Kalinin IV, Komarevtseva IO, Cherednichenko RV, et al. The biomarker significance of circulating DNA according to liquid biopsy in patients with pregnancy-associated cancer and urogenicological cancer. World of Medicine and Biology. 2025;91(1):065-071. https://doi.org/10.26724/2079-8334-2025-1-91-65-71.
12. Mathieu M, Martin-Jaular L, Lavieu G, Théry C. Specificities of secretion and uptake of exosomes and other extracellular vesicles for cell-to-cell communication. Nat Cell Biol. 2019;21(1):9-17. https://doi.org/10.1038/s41556-018-0250-9.
13. Netti GS, De Luca F, Camporeale V, Khalid J, Leccese G, Troise D, et al. Liquid Biopsy as a New Tool for Diagnosis and Monitoring in Renal Cell Carcinoma. Cancers (Basel). 2025;17(9):1442. https://doi.org/10.3390/cancers17091442.
14. Toth GG, Brodmann M, Kanoun Schnur SS, Bartus S, Vrsalovic M, Krestianinov O, et al. Intentional coronary revascularization versus conservative therapy in patients after peripheral artery revascularization due to critical limb ischemia: the INCORPORATE trial. Clin Res Cardiol. 2024;114(8):991-999. doi: 10.1007/s00392-024-02487-2.
15. van Niel G, D'Angelo G, Raposo G. Shedding light on the cell biology of extracellular vesicles. Nat Rev Mol Cell Biol. 2018;19(4):213-228. https://doi.org/10.1038/nrm.2017.125.

Publication:

«World of Medicine and Biology» Vol. 21 No. 93 (2025) , с. 88-94
УДК 616.61-006:616.65+576.367

DOI:

https://doi.org/10.26724/2079-8334-2025-3-93-88-94