EVALUATION OF INDICATORS OF BACTERIAL CONTAMINATION, ANTIMICROBIAL AND ANTIOXIDANT PROTECTION IN ORAL MUCOSAL HOMOGENATES OF RATS UNDER EXPERIMENTALLY INDUCED IMMUNODEFICIENCY AND DYSBIOSIS FOLLOWING A THERAPEUTIC-PROPHYLACTIC REGIMEN

Authors:

Abstract:

The study was devoted to the evaluation the impact of a multi-component therapeutic-prophylactic complex on indices of bacterial contamination, antimicrobial defence, and antioxidant protection in oral mucosal homogenates of rats subjected to cyclophosphamide-induced immunodeficiency and lincomycin-associated dysbiosis. Thirty adult male Wistar rats were randomly assigned to an intact control, a combined pathology group, and a combined pathology + therapeutic-prophylactic complex group (n=10 each). Seven days after modelling the pathology, animals in the third group received the therapeutic-prophylactic complex for 30 days. Urease and lysozyme activities were quantified in mucosal homogenates, and a dysbiosis index was calculated. The therapeutic-prophylactic complex significantly reduced urease activity, restored lysozyme activity, and normalised the dysbiosis index, demonstrating pronounced antimicrobial, antioxidant, and immunostimulatory effects. These findings substantiate further exploration of the therapeutic-prophylactic complex as a promising strategy for preventing complications arising from combined immunodeficient–dysbiotic conditions.

Keywords:

bacterial contamination lysozyme urease immunodeficiency dysbiosis antioxidants therapeutic-prophylactic complex

References:

1. Hubskyy YuI, Nizhenkovska IV, Korda MM. Biolohichna i bioorhanichna khimiya: u 2 kn.: pidruchnyk. Kn.2. Biolohichna khimiya. Kyiv: VSV “Medytsyna”, 2016. 497–506. [in Ukrainian].
2. Nakaz Ukrayiny “Pro zatverdzhennya Poryadku provedennya naukovymy ustanovamy doslidiv, eksperymentiv na tvarynakh”. Ministerstvo osvity i nauky Ukrayiny. 2012;249. [in Ukrainian].
3. Rohach IM, Keretsman AO, Sitkar AD. Pravylno vybranyy metod statystychnoho analizu – shlyakh do yakisnoyi interpretatsiyi danykh medychnykh doslidzhen. Naukovyy visnyk Uzhhorodskoho universytetu, seriya “Medytsyna”. 2017;2(56):124–128. [in Ukrainian].
4. Cui Y, Zhang J, Li Z, Liu X, Zhao L, Chen H, et al. Compound small peptide of Chinese medicine alleviates cyclophosphamide-induced immunosuppression by modulating Th17/Treg balance and jejunal flora in mice. Front Microbiol. 2023;14:1039287. DOI: 10.3389/fmicb.2023.1039287.
5. Di Palma G, Inchingolo AM, Latini G, Ferrante L, Nardelli P, Malcangi G, et al. The effectiveness of curcumin in treating oral mucositis: a systematic review. Nutrients. 2024;16(6):821. DOI: 10.3390/nu16060821.
6. Ferraboschi P, Ciceri S, Grisenti P. Applications of lysozyme, an innate immune defense factor, as an alternative antibiotic. Antibiotics (Basel). 2021;10(12):1534. DOI: 10.3390/antibiotics10121534.
7. Irfan M, Delgado RM, Frias-Lopez J. The oral microbiome and cancer. Front Immunol. 2020;11:591088. DOI: 10.3389/fimmu.2020.591088.
8. Kambara Y, Matsumoto T, Yamaguchi K, Fujiwara H, Yamamoto A, Gotoh K, et al. Oral dysbiosis in patients increases incidence of chronic GVHD after haploidentical transplantation. Nat Commun. 2024;15:486.
9. Kinash OV, Yeroshenko GA, Shevchenko KV, Slipchenko LB, Vatsenko AV, Riabushko OВ, et al. Metrical indices of the cecum wall in areas of gut-associated lymphoid tissuelocalisation under the in influence of food additive complex. World of Medicine and Biology. 2023;83(1):203–208. DOI: 10.26724/2079-8334-2023-1-83-203-208.
10. Malcangi G, Patano A, Ciocia AM, Netti A, Viapiano F, Palumbo I, et al. Benefits of natural antioxidants on oral health: a narrative review. Antioxidants (Basel). 2023;12(6):1309. DOI: 10.3390/antiox12061309.
11. Matsuoka M, Soria SA, Pires JR, Yamada Y, Okamoto S, Ichikawa T, et al. Natural and induced immune responses in oral cavity and saliva. BMC Immunol. 2025;26:34.
12. Ren D, Li S, Lin H, Xia Y, Li Z, Bo P, et al. Panax quinquefolius polysaccharides ameliorate antibiotic-associated diarrhoea by regulating gut microbiota in rats. J Immunol Res. 2022;2022:4126273. DOI: 10.1155/2022/4126273.
13. Wang Z, Li Y, Wang C, Xia H, Liang Y, Li Z. Oral administration of Urtica macrorrhiza polysaccharides protects against cyclophosphamide-induced intestinal immunosuppression. Exp Ther Med. 2019;18(3):2178-2186. DOI: 10.3892/etm.2019.7792.
14. Zhou X, Wu Y, Zhu Z, Lu C, Zhang C, Zeng L, et al. Mucosal immune response in biology, disease prevention and treatment. Signal Transduct Target Ther. 2025;10(1):7. DOI: 10.1038/s41392-024-02043-4.

Publication:

«World of Medicine and Biology» Vol. 21 No. 93 (2025) , с. 162-166
УДК 57.084.1:616-006.6

DOI:

https://doi.org/10.26724/2079-8334-2025-3-93-162-166