EVALUATION OF INDICATORS OF BACTERIAL CONTAMINATION, ANTIMICROBIAL AND ANTIOXIDANT PROTECTION IN ORAL MUCOSAL HOMOGENATES OF RATS UNDER EXPERIMENTALLY INDUCED IMMUNODEFICIENCY AND DYSBIOSIS FOLLOWING A THERAPEUTIC-PROPHYLACTIC REGIMEN

Authors:

Abstract:

The study was devoted to the evaluation the impact of a multi-component therapeutic-prophylactic complex on indices of bacterial contamination, antimicrobial defence, and antioxidant protection in oral mucosal homogenates of rats subjected to cyclophosphamide-induced immunodeficiency and lincomycin-associated dysbiosis. Thirty adult male Wistar rats were randomly assigned to an intact control, a combined pathology group, and a combined pathology + therapeutic-prophylactic complex group (n=10 each). Seven days after modelling the pathology, animals in the third group received the therapeutic-prophylactic complex for 30 days. Urease and lysozyme activities were quantified in mucosal homogenates, and a dysbiosis index was calculated. The therapeutic-prophylactic complex significantly reduced urease activity, restored lysozyme activity, and normalised the dysbiosis index, demonstrating pronounced antimicrobial, antioxidant, and immunostimulatory effects. These findings substantiate further exploration of the therapeutic-prophylactic complex as a promising strategy for preventing complications arising from combined immunodeficient–dysbiotic conditions.

Keywords:

bacterial contamination lysozyme urease immunodeficiency dysbiosis antioxidants therapeutic-prophylactic complex

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Publication:

«World of Medicine and Biology» Vol. 21 No. 93 (2025), pp. 162-166
UDC 57.084.1:616-006.6

DOI:

https://doi.org/10.26724/2079-8334-2025-3-93-162-166