MORPHOLOGICAL AND FUNCTIONAL CHANGES IN THE EXCHANGE VESSELS OF THE RAT ILEAL MUCOSA UNDER NITRITE ACCUMULATION INDUCED BY COMBINED ADMINISTRATION OF MONOSODIUM GLUTAMATE, SODIUM NITRITE, AND PONCEAU 4R

Authors:

Abstract:

Chemical food additives, especially when administered in combination, can induce morphofunctional changes in various organs, including the ileum. Therefore, the aim of this study was to evaluate alterations in the morphometric parameters of the exchange segment of the haemomicrocirculatory bed in the ileal mucosa of rats, as well as fluctuations in nitrite levels, following the combined administration of monosodium glutamate, sodium nitrite, and Ponceau 4R. To achieve this, histological and morphometric methods were employed, alongside the determination of nitrite concentrations in biopsies of the target organ from control animals (administered saline) and experimental groups, which received the mixture of food additives for 1, 4, 8, 12, 16, and 20 weeks. The obtained results were subjected to statistical analysis. It was established that alterations in the mean values of the total diameter, lumen diameter, and wall thickness of capillaries occurred from the early stages of the study. These parameters exhibited dynamic fluctuations, with the most pronounced changes observed in the vascular wall, which demonstrated degenerative alterations. A statistically significant increase in nitrite concentration was noted in the biochemical assessments, persisting throughout the entire duration of the experiment. Thus, it was concluded that the combined administration of chemical food additives induces marked structural and biochemical changes in the ileum.

Keywords:

small intestine haemomicrocirculatory bed capillaries food additives rats mucosa nitrites morphometry

References:

1. Akimov OY, Kostenko VO. Okyslyuvalno-nitrozatyvnyy stres ta metody yoho doslidzhennya. Lviv: Mahnoliya; 2021.152 s. [in Ukrainian].
2. Horalskyy LP, Khomych VT, Kononskyy OI. Osnovy histolohichnoyi tekhniky i morfofunktsionalni metody doslidzhen u normi ta pry patolohiyi. Zhytomyr: «Polissya»; 2015. 286 s. [in Ukrainian].
3. Akimov OY, Kostenko VO. Functioning of nitric oxide cycle in gastric mucosa of rats under excessive combined intake of sodium nitrate and fluoride. Ukr Biochem J. 2016;88(6):70–5. DOI: https://doi.org/10.15407/ubj88.06.070.
4. Amtul Z, Yang J, Lee TY, Cechetto DF. Pathological Changes in Microvascular Morphology, Density, Size and Responses Following Comorbid Cerebral Injury. Front Aging Neurosci. 2019;11:47. DOI: 10.3389/fnagi.2019.00047.
5. Bilash SM, Oliinichenko YaO, Pronina OM, Koptev MM, Pirog-Zakaznikova AV, Donchenko SV, et al. Reaction of the capacitive link of the hemomicrocirculatory bed of the ileum under oxidative stress caused by the introduction of a complex of chemical food additives. World of Medicine and Biology. 2025;1(91):145-149. DOI: 10.26724/2079-8334-2025-1-91-145-149.
6. Cicchetti A, Laurino F, Possenti L, Rancati T, Zunino P. In silico model of the early effects of radiation therapy on the microcirculation and the surrounding tissues. Phys Med. 2020 May;73:125-134. DOI: 10.1016/j.ejmp.2020.04.006.
7. Kahe K, Laferrere B, Castellanos FX, Zhang Y, Mozaffarian D. Monosodium glutamate: A hidden risk factor for obesity? Obes Rev. 2025;26(6):e13903. DOI: https://doi.org/10.1111/obr.13903.
8. Liu L, Wen L, Gao C, Piao H, Zhao H, Yu D, et al. Effects of Non-directional Mechanical Trauma on Gastrointestinal Tract Injury in Rats. Front Physiol. 2021 Apr 15;12:649554. DOI: 10.3389/fphys.2021.649554.
9. Moradi A, Mokhtarpour A, Yazdani A, Kianersi K, Bahari Khasraghi L. Algorithmic Approach to Inflammatory Disorders of Ileum. Iran J Pathol. 2022 Fall;17(4):381-394. DOI: 10.30699/IJP.2022.539357.2736.
10. Niethamer TK, Levin LI, Morley MP, Babu A, Zhou S, Morrisey EE. Atf3 defines a population of pulmonary endothelial cells essential for lung regeneration. Elife. 2023 May 26;12:e83835. DOI: 10.7554/eLife.83835.
11. Popko SS. Morphological rearrangement of the metabolic link of the microcirculatory bed of guinea pigs lungs after sensitization with ovalbumin. Current issues in pharmacy and medicine: science and practice. 2021;14(1):79-83. DOI: https://doi.org/10.14739/2409-2932.2021.1.226851.
12. Savchuk RV, Kostyev FI, Zhukovskij DA, Nasibullin BA. Structural and functional transformation of the small intestine wall in the conditions of the functioning of the artificial urinary reservoir in the experiment. Reports of Morphology. 2018;24(1):28-33. DOI: 10.31393/morphology-journal-2018-24(1)-05.
13. Synenko VA, Yeroshenko GA, Shevchenko KV, Grygorenko AS, Sokolenko VV, Sharlay NM, et al. Current views on the impact of sodium nitrite and Ponceau 4R food additives on the retina and the whole body. World of Medicine and Biology. 2024;4(90):260-264. DOI: https://doi.org/10.26724/2079-8334-2024-4-90-260-264.
14. Yeroshenko GA, Donets IM, Shevchenko KV, Vatsenko АV, Ulanovska-Tsuba NA, Riabushko OB, et al. Morphometric and morphological features of rat bronchus-associated lymphoid tissue under the impact of the complex of food additives. World of Medicine and Biology. 2023;3(85):209-215. DOI: https://doi.org/10.26724/2079-8334-2023-3-85-209-215.
15. Yeroshenko GA, Grygorenko AS, Shevchenko KV, Lysachenko OD, Riabushko OB, Pyvovar NM, et al. Influence of food additives complex on the morphology of villi of the rats’ duodenum mucosa. World of Medicine and Biology. 2022;2(80):199-203. DOI: https://doi.org/10.26724/2079-8334-2022-2-80-199-203.

Publication:

«World of Medicine and Biology» Vol. 21 No. 93 (2025) , с. 188-192
УДК 616.344:611.1:612.08:599.323.4:54

DOI:

https://doi.org/10.26724/2079-8334-2025-3-93-188-192