FEATURES OF RAT BLOOD SERUM MARKERS AGAINST THE BACKGROUND OF FLUOROSIS MODELING, ORTHODONTIC INTERVENTION, AND THERAPEUTIC AND PROPHYLACTIC COMPLEX

Authors:

Abstract:

The study was devoted to evaluate the influence of a therapeutic-prophylactic complex on blood-serum markers in experimental animals under conditions of modelled fluorosis and orthodontic intervention. Experimental studies were carried out on 40 male Wistar rats (herd breeding), 4 months of age, with an average body mass of 280±14 g. The developed therapeutic-prophylactic complex exhibits pronounced antioxidant and anti-inflammatory (adaptogenic) properties. Its application enhances non-specific resistance under the combined effects of fluoride excess and mechanical stress, as evidenced by normalisation of biochemical markers of oxidative stress and proteolysis. These findings substantiate the feasibility of employing this complex to prevent oxidative damage and inflammatory changes in clinical practice, particularly in orthodontic patients residing in endemic fluorosis areas.

Keywords:

fluoride fluorosis blood serum rats experimental study

References:

1. Hubskyy YUI, Nizhenkovska IV, Korda MM. Biolohichna i bioorhanichna khimiya: u 2 kn.: pidruchnyk. Kn.2. Biolohichna khimiya. Kyiv: VSV “Medytsyna”, 2016. 497–506. [in Ukrainian].
2. Nakaz Ukrayiny “Pro zatverdzhennya Poryadku provedennya naukovymy ustanovamy doslidiv, eksperymentiv na tvarynakh”. Ministerstvo osvity i nauky Ukrayiny. 2012;249. [in Ukrainian].
3. Rohach IM, Keretsman AO, Sitkar AD. Pravylno vybranyy metod statystychnoho analizu – shlyakh do yakisnoyi interpretatsiyi danykh medychnykh doslidzhen. Naukovyy visnyk Uzhhorodskoho universytetu, seriya “Medytsyna”. 2017;2(56):124–128. [in Ukrainian].
4. Burster T, Mustafa Z, Myrzakhmetova D, Zhanapiya A, Zimecki M. Hindrance of the proteolytic activity of neutrophil-derived serine proteases by serine protease inhibitors as a management of cardiovascular diseases and chronic inflammation. Front Chem. 2021;9:784003. DOI: 10.3389/fchem.2021.784003.
5. Inchingolo F, Inchingolo AM, Latini G, Ferrante L, Trilli I, Del Vecchio G, et al. Oxidative stress and natural products in orthodontic treatment: A systematic review. Nutrients. 2024;16(1):113. DOI: 10.3390/nu16010113.
6. Li M, Wang J, Wu P, Manthari RK, Zhao Y, Li W, et al. Self-recovery study of the adverse effects of fluoride on small intestine: Involvement of pyroptosis induced inflammation. Sci Total Environ. 2020;742:140533. DOI: 10.1016/j.scitotenv.2020.140533.
7. Pal P, Jha NK, Pal D, Jha SK, Anand U, Dey A, et al. Molecular basis of fluoride toxicities: Beyond benefits and implications in human disorders. Genes Dis. 2023;10(4):1470–1493. DOI: 10.1016/j.gendis.2022.09.004.
8. Shashi A, Thakur S. Gene expression and alterations of antioxidant enzymes in spleen of rats exposed to fluoride. J Trace Elem Med Biol. 2022;72:126966. DOI: 10.1016/j.jtemb.2022.126966.
9. Song C, Zhao J, Fu B, Li D, Mao T, Peng W, et al. Melatonin-mediated upregulation of Sirt3 attenuates sodium fluoride-induced hepatotoxicity by activating the MT1-PI3K/AKT-PGC-1α signaling pathway. Free Radic Biol Med. 2017;112:616–630. DOI: 10.1016/j.freeradbiomed.2017.09.005.
10. Xia J, Zhang X, Xu L, Yan N, Sun Z, Duan X, et al. Sodium butyrate attenuates oxidative stress, apoptosis, and excessive mitophagy in sodium-fluoride-induced hepatotoxicity in rats. Ecotoxicol Environ Saf. 2025;291:117821. DOI: 10.1016/j.ecoenv.2025.117821.

Publication:

«World of Medicine and Biology» Vol. 21 No. 92 (2025) , с. 202-206
УДК [57.084.1+546.16]:616.31-08-039.71

DOI:

https://doi.org/10.26724/2079-8334-2025-2-92-202-206