CHARACTERISTICS OF METRIC PARAMETERS OF THE ILEUM WALL IN RATS UNDER LONG-TERM COMPLEX INFLUENCE OF CHEMICAL FOOD ADDITIVES

Authors:

Abstract:

It has been experimentally proven that chemical food additives can cause changes in the morphological structure of various internal organs, including the digestive system. To date, data on the complex effects of these substances on the ileum are rather limited. It justified the aim of the study, which was to investigate changes in the intestinal wall after long-term administration of a complex of chemical food additives, including monosodium glutamate, sodium nitrite and ponceau 4R. In this study, using histological and morphometric methods of research, we determined the dynamic changes in the overall average wall thickness and its separate layers. It was found that the ileum of rats of both the control group (receiving saline) and the experimental groups (receiving a complex of chemical food substances for 1, 4, 8, 12, 16, and 20 weeks) is represented by serosa, muscular layer, submucosa and mucosa. It has been established that changes occur for up to 8 weeks due to hyperhydration of connective tissue and pericellular oedema of the structural components of all layers. However, starting from week 12, there are signs of irreversible changes that progress to week 20 and manifest as dystrophic changes with subsequent atrophy of the ileal wall, confirmed by a decrease in all indicators. Thus, with their prolonged administration, chemical food additives disrupt the protective and adaptive mechanisms of the ileum.

Keywords:

ileum intestinal wall food additives morphometric analysis rats mucosa serosa lymphocytes

References:

1. Horalskyy LP, Khomych VT, Kononskyy OI. Osnovy histolohichnoyi tekhniky i morfofunktsionalni metody doslidzhen u normi ta pry patolohiyi. Zhytomyr: “Polissya”; 2015. 286 s. [in Ukrainian].
2. Oliynichenko YaO. Metrychni zminy stinky klubovoyi kyshky shchuriv pry diyi kompleksu kharchovykh khimichnykh dobavok na rannikh terminakh eksperymentu. Aktualni problemy suchasnoyi medytsyny. 2024;24(2(86)):131–136. doi: https://doi.org/10.31718/2077-1096.24.2.131. [in Ukrainian].
3. Shepitko KV. Kharakterystyka morfometrychnykh parametriv stinky klubovoyi kyshky pry odnorazovomu vvedenni kriokonservovanoyi platsenty ta aseptychnomu zapalenni. Svit medytsyny ta biolohiyi. 2014;4(46):174–178. [in Ukrainian].
4. Akimov OY, Kostenko VO. Functioning of nitric oxide cycle in gastric mucosa of rats under excessive combined intake of sodium nitrate and fluoride. Ukrainian Biochemical Journal. 2016;88(6):70–5. doi: 10.15407/ubj88.06.070.
5. Banerjee A, Mukherjee S, Maji BK. Monosodium glutamate causes hepato-cardiac derangement in male rats. Hum Exp Toxicol. 2021;40:359–369. doi: 10.1177/09603271211049550.
6. Banerjee A, Mukherjee S, Maji BK. Worldwide flavor enhancer monosodium glutamate combined with high lipid diet provokes metabolic alterations and systemic anomalies: an overwiev. Toxicology Reports. 2021; 8:938–961. doi: 10.1016/j.toxrep.2021.04.009.
7. Grigorenko А, Yeroshenko G, Shevchenko K, Lisachenko O, Perederii N. Remodeling of the rat duodenal wall under the effect of complex food additives of monosodium glutamate, sodium nitrite and Ponceau 4R. Georgian Medical News. 2021;5(314):145–150.
8. Koka VM, Starchenko II, Mustafina GM, Roiko NV, Filenko BM, Proskurnia SA. Features of the structure of the epithelium of the mucous membrane of the tongue under the effect of complex of food additives in the experiment. World of medicine and biology. 2022;1(79):200–204. doi: 10.26724/2079-8334-2022-1-79-200-204.
9. Lavado G, Higuero N, León-Camacho M, Cava R. Formation of Lipid and Protein Oxidation Products during In Vitro Gastrointestinal Digestion of Dry-Cured Loins with Different Contents of Nitrate/Nitrite Added. Foods. 2021; 10:1748. doi: https://doi.org/10.3390/foods10081748.
10. Song Z, Song R, Liu Y, Wu Z, Zhang X. Effects of ultra-produced foods on the microbiota-gut-brain axis: The bread-and-butter issue. Food Res Int. 2023;167:112730. doi: 10.1016/j.foodres.2023.112730.
11. Yeroshenko GA, Donets IM, Shevchenko KV, Vatsenko AV, Ulanovska-Tsyba NA, Rіabushko OB, et al. Morphometric and morphological features of rat bronchusassociated lymphoid tissue under the impact of the complex of food additives. World of medicine and biology. 2023;3(85):209–215. doi: 10.26724/2079-8334-2023-3-85-209-215.

Publication:

«World of Medicine and Biology» Vol. 20 No. 89 (2024) , с. 203-207
УДК 611.344:613.29-036.14-071.3-092.9

DOI:

https://doi.org/10.26724/2079-8334-2024-3-89-203-207