| About the author: |
Kornienko V. V. |
| Heading |
EXPERIMENTAL MEDICINE |
| Type of article |
Scentific article |
| Annotation |
The aim of our research was to evaluate effectiveness of chitosan films that are applied to treat burns. We studied the features of tissue morphogenesis of the thermal damaged skin in the young animals. We modeled the burn wounds of IIIb degree on the rats of the experimental and control groups. The chitosan films were applied on the animals of the experimental group for the topical treatment. We analyzed the healing of burn wounds by the morphological and cytological criteria. We measured them on 1st, 3rd, 7th, 14th, and 21st days after the burn modelling. Application of the chitosan films to treat thermal burns enhanced wound cleaning from dead tissue and reduced eschar, decreased the intensity of inflammatory reactions and disorders of blood circulation, stimulated the early growth of granulation tissue, and improved epithelialization of the wound. |
| Tags |
burn, medical supplies, chitosan, morphometry, cytology |
| Bibliography |
- Автандилов Г. Г. Основы количественной патологической анатомии / Г. Г. Автандилов // М.: Медицина. – 2002. – 239 с.
- Быков В. Л. Цитология и общая гистология (функциональная морфология клеток и тканей) / В. Л. Быков // – СПб.: СОТИС, - 2003. – 520 с.
- Камаев М. Ф. Инфицированная рана и ее лечение. 2-е изд. / М. Ф. Камаев // - М.: Медицина, - 1979. - 159 с.
- Кузин М. И. Раны и раневая инфекция: Руководство для врачей / М. И. Кузин, Б.М. Костюченок // – М.: Медицина. – 1990. – 592 с.
- Коржевский Д. Э. Основы гистологической техники / Д.Э. Коржевский, А. В. Гиляров // СПб.: СпецЛит.-2010 95 c.
- Покровская М. П. Цитология раневого экссудата как показатель заживления ран / М. П. Покровская, М. С. Макаров // М.: Медгиз, - 1942. – 44 с.
- Azad A. K. Chitosan membrane as a wound-healing dressing: characterization and clinical application / A.K. Azad, N. Sermsintham, S. Chandrkrachang [et al.] // J Biomed Mater Res Appl Biomater. – 2004. – Vol. 69B, P.216-222.
- Bottomley M. K. Metalloproteinases as targets for anti-Inflammatory drugs / M.K. Bottomley, D. Bradshaw, J.S. Nixon [et al.] // Basel: Birkhauser. – 1999.
- Boucard N. The use of physical hydrogels of chitosan for skin regeneration following third-degree burns / N. Boucard, C. Vitona, D. Agayb [et al.] // Biomaterials – 2007. – Vol. 28, P.3478 – 3488.
- Chen R. N. Development of N, O-(carboxymethyl) chitosan/collagenmatrixes as awound dressing / R.N. Chen, G.M. Wang, C.H. Chen [et al.] // Biomacromolecules. – 2006. – Vol. 7, P. 1058 –1064.
- HaiPeng G. Studies on nerve aftinity of chitosan-derived materials / G. HaiPeng, Z. Yinghui, L. Jianchun [et al.] // Journal of Biomedical Material Research. – 2000. – Vol. 52(2). – P. 285–295
- Ignatova M. Electrospun nano-fibre mats with antibacterial properties from quaternized chitosan and poly(vinyl alcohol) / M. Ignatova, K. Starbova, N. Markova [et al.] // Carbohydr Res. – 2006. – Vol. 341, P. 2098 – 2107.
- Masayuki Photocrosslinkable chitosan as a dressing for wound occlusion and accelerator in healing process / Masayuki, Ishihara, Kuniaki [et al.] // Biomaterials. – 2002. – Vol. 23(5). – P. 833–840.
- Midwood K.S. Tissue repair and the dynamics of the extracellular matrix / K.S. Midwood, L.V. Williams, J.E. Schwarzbauer // The International Journal of Biochemistry & Cell Biology. – 2004. – Vol. 36 (6), P.1031–1037.
- Pollock The cellular, biochemical, and mechanical phases of wound healing / Pollock, E. Raphael, F. Charles [et al.] // Schwartz's Principles of Surgery, Ninth Edition. McGraw-Hill Professional. – 2009.
- Slack J. M. Regeneration research today / J. M. Slack // Dev. Dyn. –2003. – P. 162–166.
- Vats A. Scaffolds and biomaterials for tissue engineering: a review of clinical applications / A.Vats // Clin. Otolaryngol. Allied Sci. – 2003. – P. 165–172/
- Willoughby D. A. Inducible enzymes in the inflammatory response / D.A. Willoughby, A. Tomlinson [et al.] // Basel, CH: Birkhauser Verlag. – 1999.
- Watthanaphanit A. Fabrication, structure, and properties of chitin whisker-reinforced alginate nanocomposite fibers / A. Watthanaphanit, P. Supaphol, H. Tamura [et al.] // Appl Polym Sci. – 2008. – Vol.110, Р. 890–899.
- Wang C. C. Water absorbing and antibacterial properties of N-isopropyl acrylamide grafted and collagen/chitosan immobilized polypropylene nonwoven fabric and its application on wound healing enhancement / C.C. Wang, C.H. Su, C.C. Chen // J Biomed Mater Res. – 2008. – Vol.84A, P. 1006–1017.
- Yu-Bey Preparation and characterization on mechanical and antibacterial properties of chitosan/cellulose blends / Yu-Bey, Wu, Shu-Huei [et al. ] // Carbohydrate Polymers. – 2004. – Vol. 57(7). – P. 435–440.
- Yang L. Identification of fibrocytes in postburn hypertrophic scar / L. Yang, P. G. Scott , C. Dodd [et al.] // Wound Repair Regen. – 2005 – 13, Р. 398–404.
|
| Publication of the article |
«World of Medicine and Biology» №4(46) 1 part 2014 year, 107-113 pages, index UDK 616-001.17-003.93-089.42: 547.995: 616-092.9-053.81 |
