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    Pravotorova O. S., Silkina Yu. V.

    METHODOLOGY OF LABELING DENDRITIC CELLS IN THE COLONIC MUCOSA IN EXPERIMENTAL ULCERATIVE COLITIS


    About the author: Pravotorova O. S., Silkina Yu. V.
    Heading EXPERIMENTAL MEDICINE
    Type of article Scentific article
    Annotation There is a problem - labeling of immune cells with monoclonal antibodies to CD-receptors because appears the cross expression of cell determinants by several types of leukocytes, that requires to use of two or three markers for identification some cell clusters. No exception the dendritic cells that have identical antigenic tags the same the histiocytes, B-lymphocytes. The aim - to find a specific marker of dendritic cells within the inflamed mucosa of the colon in experimental ulcerative colitis. We were studied colon laboratory Wistar rats, which modeled ulcerative colitis by administering trinitrobenzolsulfonic acid in the colon. It was found that the most important marker of dendritic cells in the mucosal part of the colon is S-100 because accumulates in the cytoplasm dendritic cells, but not leukocytes and histiocytes, not requires differentiation with neural elements. Marker allows to investigate the details of the heterogeneity of its accumulation in the cytoplasm of dendritic cells.
    Tags dendritic cells, ulcerative colitis, immunohistochemical markers
    Bibliography
    • Глузман Д. Ф. Диагностика опухолей из гистиоцитов и дендритных клеток / Д. Ф. Глузман, Л. М. Скляренко, С. В. Коваль [и др.] // Здоров’я України. – 2013. - № 9. – С. 32-34.
    • Дорофеев А. Э. Сравнительная оценка клинических форм и степени активности воспалительных заболеваний кишечника / А. Э. Дорофеев, А. А. Элина // Новости медицины и фармации. Гастоэнтерология. – 2012. - № 407. –4 с.
    • Одинец А. Г. Роль дендритных клеток в иммунном ответе / А. Г. Одинец // Новые медицинские технологии. – 2008. - № 8. – С. 72-74.
    • Fries P. Mucosal dendritic cell diversity in the gastrointestinal tract / P. Fries, P. Griebel // Cell Tissue Res.– 2011. - № 343. – Р. 33-41.
    • Olson O. Homeostasis and inflammation in the Intestine / O. Olson, J. Laukoetter, E. Su [et al.] //Cell. – 2010. - № 6. – Р. 859-870.
    • Tolstanova G. Role of anti-angiogenic factor endostatin in the pathogenesis of experimental ulcerative colitis / G. Tolstanova, X. Deng, T. Khomenko [et al.] // Life Sci. – 2011. – Vol. 88 (1-2). – P. 74-81.
    Publication of the article «World of Medicine and Biology» №4(46) 1 part 2014 year, 143-145 pages, index UDK 616.345-002-092.4:576:31:615.37