Rikalo N. A., Polinkevich S. G.

FEATURES NUCLEAR DNA PLOIDY OF HEPATOCYTES IN RATS OF DIFFERENT AGES UNDER CONDITIONS OF CHRONIC TOXIC HEPATITIS

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About the author:	Rikalo N. A., Polinkevich S. G.
Heading	EXPERIMENTAL MEDICINE
Type of article	Scentific article
Annotation	Nuclear DNA ploidy was determined by the usage of flow cytometry at the rats of different age groups with chronic toxic hepatitis. Revealed that the group of animals with chronic toxic hepatitis significantly bigger amount of the hepatocytes with polyploid DNA in comparison to the intact animals of similar age. In our opinion, this feature changes of the ratio of hepatocytes nuclei with different ploidy can be explained by the fact that the cells with octaploid and dyoctaploid set of nuclear DNA are a reserve pool, and after termination of the pathogenic factor action re-enter to the cell cycle and become a source of formation of diploid cells because regeneration by proliferation of this type hepatocytes is the main mechanism of mass recovery, the structure and function of the damaged liver.
Tags	ploidy, hepatitis, age characteristics
Bibliography	Celton-Morizur S. Polyploidization of liver cells / S. Celton-Morizur, C. Desdouets // Advances in Experimental Medicine and Biology. -2010. – Vol. 676. –P. -123-135. Guidotti J. E. Liver Cell Polyploidization: A Pivotal Role for Binuclear Hepatocytes / J. E. Guidotti, O. Brégerie, A. Robert [et al] // The journal of biological chemistry. -2003. - Vol. 278. - No. 21. - P. 19095–19101 Margall-Ducos G. Liver tetraploidization is controlled by a new process of incomplete cytokinesis / G. Margall-Ducos, S. Celton-Morizur, C. Dominique [et al.] // Journal of Cell Science. – 2007. - Vol. 120. - №20. - P. 3633-3639. Ravid K. Roads to polyploidy: the megakaryocyte example. / K. Ravid, J. Lu, J. M. Zimmet [et al.] // Journal of Cellular Physiology.- 2002. – Vol.190(1). - P. 7-20. Torres S. Thyroid hormone regulation of rat hepatocyte proliferation and polyploidization / S. Torres, B.P. Díaz, J.J. Cabrera [et al.] // American Journal of Physiology - Gastrointestinal and Liver Physiology.- 1999. - Vol.276. -P.155-163
Publication of the article	«World of Medicine and Biology» №4(47) 2 part 2014 year, 183-186 pages, index UDK 616.36-002:599.323.4:601.2:575.853