РусскийEnglishУкраїнська
  • Main
  • Useful links
  • Information for Contributors
  • About
  • Editorial board

  • Article
    Gancho O.V., Loban G.A., Vazhnichaya Ye.M., Skripnik N.V.

    ANTIMICROBIAL ACTION OF COMPOSITIONS BASED ON SILVER NANOPARTICLES AND 3-HYDROXYPYRIDINE DERVATIVE


    About the author: Gancho O.V., Loban G.A., Vazhnichaya Ye.M., Skripnik N.V.
    Heading EXPERIMENTAL MEDICINE
    Type of article Scentific article
    Annotation Among the promising tools in the market of new antimicrobial agents associated with nanotechnology one of the first position is occupied by nanoparticles (NPs) of silver, which have a wide range of antibacterial, antiviral and antiparasitic activity. According to current data regardless of the method of preparation, silver nanoparticles (NPs) have antibacterial and antifungal activity, the potency of which depends on the size, nature and condition of the surface coating. This effect is due to the release of silver ions and the formation of the interface defined between NPs and the cell. Nanosilver antimicrobial effects are more pronounced against gram-negative microflora and are propagating on biofilm- and spore forming strains, as well as on bacteria multi-resistant to antibiotics. As a result of studying the sensitivity museum strains of S. aureus ATCC 25923, S. epidermidis ATCC 14990, E. coli ATCC 25922, E. faecalis ATCC 29212, C. albicans ATCC 10231 to silver (Ag) nanoparticles obtained by electron-ray technology and dispersed in hydrophilic medium with the use of 3-hydroxypyridine derivative and polyvinylpyrrolidone (PVP) or dextran, it was shown that such dispersions exhibit antimicrobial activity against E. coli ATCC 25922. Among them liquid which is composed of Ag nanoparticles together with 3-hydroxypyridine derivative and PVP has the greatest bacteriostatic action. The aim of this study was to determine the antimicrobial properties of disperse systems based on various combinations of silver nanoparticles and condensate derivative 3- hydroxypyridine to the bacteria strains isolated from the oral cavity of healthy students. It is studied the sensitivity of strains isolated from oral cavity of students to silver nanoparticles (NPs) obtained by electron-beam technology and dispersed in hydrophilic medium with the use of 2-ethyl-6-methyl-3-hydroxypyridine succinate and polyvinyl pyrrolidone or dextran. Investigated the sensitivity of strains of microorganisms isolated from the oral fluid student volunteers to nanoparticles (NPs) of silver obtained by electron-beam technology, and dispersed in a hydrophilic medium with use of a 2-ethyl-6-methyl-3-hydroxypyridine succinate and polyvinyl pyrrolidone or dextran. It is shown that S. aureus, S. epidermidis, E. faecalis, Neisseria sp. and Bacillus sp. are sensitive to all studied samples of stabilized silver NPs. This sensitivity depends on the microorganism species, the nature of NPs coating and silver concentration. It indicates the prospects to use of silver NPs with different surface characteristics in dental diseases of infective-inflammatory genesis.
    Tags sensitivity of microorganisms, nanoparticles, silver
    Bibliography
    • Gancho O. V. Chutlivіst' muzejnih shtamіv mіkroorganіzmіv do preparatіv na osnovі kondensatu іz nanochastinok srіbla / O.V. Gancho, G.A. Loban', O.M. Vazhnicha [ta іn.] // Vіsnik problem bіologії ta medicini. – 2014. – № 1(43). – S.102-105.
    • Movchan B.O. Antibakterіal'na aktivnіst' novogo farmacevtichnogo іngredієnta – nanokompozicії srіbla / B.O. Movchan, І.S. Chekman, S.B. Bіlous [ta іn.] // Profіlaktichna medicіna. – 2014. – №1-2. – C. 7-14.
    • Nakaz MOZ Ukraїni za №167 vіd 05.04.2007 r. «Pro zatverdzhennja metodichnih vkazіvok «Viznachennja chutlivostі mіkroorganіzmіv do antibakterіal'nih preparatіv».– [Elektronnij resurs].– Rezhim dostupu: http://www.moz.gov.ua
    • Pat. 75670 UA; MPK B01J 13/00, C23C 14/24, B82B 3/00. Sposіb oderzhannja koloїdnogo rozchinu srіbla / B.O. Movchan (UA); Z.R. Ul'berg (UA); V.P. Chernih (UA); V.A. Prokopenko (UA); S.M. Kovalenko (UA); G.G. Dіdіkіn (UA). – №u201206263; zajavl. 24.05.2012; opubl. 10.12.2012, bjul. №23/2012.
    • Pat. 92556 UA, MPK B82B 3/00, C23C 14/24, C23C 14/54. Sposіb oderzhannja nanochastinok sistemi metal-kisen' іz zadanim skladom elektronno-promenevim viparovuvannjam і kondensacієju u vakuumі / B.Є. Paton (UA), B.O. Movchan (UA), Ju.A. Kurapov (UA), K.Ju. Jakovchuk (UA); zajavnik ta patentovlasnik Astromont Limited (CY). – №a200905985; zajavl. 10.06.2009; opubl. 10.11.2010, bjul. №21.
    • Savchenkova L. І. Problema antibiotikorezistentnosti v sovremennoj medicine / L. І. Savchenkova, V. O. Luk'janchuk // Vіsnik farmakologії ta farmacії. – 2005. – №1. – S. 43-49.
    • Feshhenko Ju. I. Racional'naja antibiottikoterapija bol'nyh s infekcijami nizhnih dyhatel'nyh putej / Ju.I. Feshhenko, A.Ja. Dzjublik / II Mіzhnarodnij kongres z antiіnfekcіjnoї hіmіoterapії, 10-11 grudnja 2009 roku, Kiїv: materіali kongresu. – Ukraїns'kij pul'monologіchnij zhurnal, – 2009. – №4. – C. 5-8.
    • Chekman І. S. Nanonauka, nanobіologіja, nanofarmacіja: monografіja / І. S. Chekman, Z. R. Ul'berg, V. O. Malanchuk [ta іn.]. – K.: Polіgraf pljus, 2012. – 328 s.
    • Shherbakov O. B. Preparati srіbla: vchora, s'ogodnі і zavtra / O. B. Shherbakov, G.І. Korchak, O.V. Surmasheva [ta іn.] // Farmacevtichnij zhurnal. – 2006. – №5. – S. 45-57.
    • Chernousova S. Silver as antibacterial agent: ion, nanoparticle, and metal / S. Chernousova, M. Epple // Angew. Chem. Int. Ed. Engl. – 2013. – Vol. 52, №6. – P. 1636-1653.
    • Di Giulio M. The effect of a silver nanoparticle polysaccharide system on streptococcal and saliva-derived biofilms / M. Di Giulio, S. Di Bartolomeo, E. Di Campli [et al.] // Int. J. Mol. Sci. – 2013. – Vol. 14, №7. – R.13615-13625.
    • Jain J. Silver nanoparticles in therapeutics: development of an antimicrobial gel formulation for topical use / J. Jain, S. Arora, J.M. Rajwade [et al.] // Mol. Pharm. – 2009. – Vol. 66, №5. – P. 1388-1401.
    • Mei L. Bioconjugated nanoparticles for attachment and penetration into pathogenic bacteria / L. Mei, Z. Lu, W. Zhang [et al.] // Biomaterials. – 2013. –Vol. 34, №38. – P. 10328-10337.
    • Rizzello L. Nanotechnology tools for antibacterial materials / L. Rizzello, R, Cingolani, P.P. Pompa // Nanomedicine (Lond.). – 2013. –Vol. 8, №5. – P. 807-821.
    Publication of the article «World of Medicine and Biology» №1(48), 2015 year, 113-116 pages, index UDK 579.22:546.57