EnglishУкраїнська
  • Main
  • Useful links
  • Information for Contributors
  • About
  • Editorial board

  • Article
    Zabenko Y. Y., Pivneva T. A.

    EFFECTS OF REPETITIVE MILD TRAUMATIC BRAIN INJURY ON BEHAVIOUR OF MICE IN THE “OPEN FIELD”


    About the author: Zabenko Y. Y., Pivneva T. A.
    Heading EXPERIMENTAL MEDICINE
    Type of article Scentific article
    Annotation Mild traumatic brain injury, often identified with brain concussion, is about 80% of all cases of traumatic brain injury. Due to insufficient sensitivity of standard diagnostic methods pathophysiology of mild traumatic brain injury remains poorly understood. Until recently, there was information only about external manifestations of concussion, such as loss of consciousness, amnesia, sleep disorders, emotional instability, etc. Although mild trauma is not considered to be life threatening in close perspective, the evidence of its delayed neurodegenerative effects is growing. The aim of our research was to study changes in behavior of mice after experimental repetitive mild traumatic brain injury. To induce trauma, we used a new model, developed by Michael J. Kane et al. Kane’s design represents a modification of Marmarou’s weight-drop injury. The objects were 32 males (age – 2-3 months) of white laboratory mouse (24-40 g). Animals were divided into following groups: 1) non-anesthetized, non-injured; 2) anesthetized, non-injured; 3) anesthetized, injured. Comparison of righting reflex duration in groups of anesthetized non-injured and anesthetized injured mice revealed delay in full recovery of consciousness. In the “Open field”, we tested mice for locomotor activity, rest duration, grooming activity and anxiety level on day. When conducting the test in injured animals we observed a decrease of locomotor activity and increase of anxiety level at days 5 and 10 since the first impact and recovery of respective characteristics to the level of control at day 30. Delay in recovery of consciousness together with increase of anxiety level indicate symptoms related to concussion in humans. Thus, the new model might be useful for further studies of neurodegenerative processes induced by this kind of trauma and for selection of potential treatments.
    Tags repetitive mild traumatic brain injury, behavioral tests
    Bibliography
    • Belanger H. G. Factors moderating neuropsychological outcomes following mild traumatic brain injury: A meta-analysis / H. G. Belanger, G. Curtiss, J. A. Demery [et al.] // J Int Neuropsychol Soc. – 2005. – Vol. 3, N. 11. – P. 215–227.
    • Cassidy J. D. Incidence, risk factors and prevention of mild traumatic brain injury: results of the WHO collaborating centre task force on mild traumatic brain injury / J. D. Cassidy, L. J. Carroll, P. M. Peloso [et al.] // J. Rehabil. Med. – 2004. – N. 36. – P. 28–60.
    • Dixon C. E. A fluid percussion model of experimental brain injury in the rat / C. E. Dixon, B. G. Lyeth, J. T. Povlishock [et al.] // J. Neurosurg. – 1987. – Vol. 1, N. 67. – P. 110–119.
    • Davis G. A. Contributions of neuroimaging, balance testing, electrophysiology and blood markers to the assessment of sport-related concussion / G. A. Davis, G. L. Iverson, K. M. Guskiewicz [et al.] // Br. J. Sports Med. – 2009. – N. 43. – P. 36–45.
    • Daneshvar D. H. Long-term consequences: effects on normal development profile after concussion / D. H. Daneshvar, D. O. Riley, C. J. Nowinski [et al.] // Phys. Med. Rehabil. Clin. N. Am. – 2011. – Vol. 4, N. 22.– P. 683–700.
    • Daneshvar D. H. The epidemiology of sport-related concussion / D. H. Daneshvar, C. J. Nowinski, A. C. McKee [et al.] // Clin. Sports Med. – 2011. – Vol. 1, N. 30. – P. 1‑17.
    • Kane M. J. / A mouse model of human repetitive mild traumatic brain injury / M. J. Kane, M. Angoa-Pérez, D. I. Briggs [et al.] // J. Neurosci Methods. – 2012. – Vol.1, N. 203. – P. 41-49.
    • Lighthall J. W. Controlled cortical impact: a new experimental brain injury model / J. W. Lighthall // J. Neurotrauma. – 1988. – Vol. 1, N. 5. – 1–15.
    • Lundin A. Symptoms and disability until 3 months after mild TBI / A. Lundin, C. de Boussard, G. Edman [et al.] // Brain Inj. – Vol. 8, N. 20. – 2006. – 799–806.
    • Lehman E. J. / Neurodegenerative causes of death among retired National Football League players / E. J. Lehman, M.J.Hein, S. L. Baron [et al.] // Neurology. – 2012. – Vol. 19, N. 79. – P. 1970–1974.
    • Marmarou A. A new model of diffuse brain injury in rats. Part I: Pathophysiology and biomechanics / A. Marmarou, M. A. Foda, W. van den Brink [et al.] // J.Neurosurg. – 1994. – Vol. 2, N. – 291– 300.
    • McKee A. C. The neuropathology of sport / A. C. McKee, D.H. Daneshvar, V. E. Alvarez [et al.] // Acta Neuropathol. – 2014. – Vol. 1, N. 127. – 29–51.
    • Schneiderman A. I. Understanding sequelae of injury mechanisms and mild traumatic brain injury incurred during the conflicts in Iraq and Afghanistan: persistent postconcussive symptoms and posttraumatic stress disorder / A. I. Schneiderman, E. R. Braver, H.K. Kang // Am. J. Epidemiol. – 2008. – Vol. 12, N. 167. – 1446–1452.
    • Stern R. A. Long-term consequences of repetitive brain trauma: chronic traumatic encephalopathy / R. A. Stern, D. O. Riley, D. H. Daneshvar [et al.] // PM & R. – 2011. – N. 3. – 460–46.
    Publication of the article «World of Medicine and Biology» №1(48), 2015 year, 122-125 pages, index UDK 616-001: 57.083.3