| About the author: |
I. Zastavnyy, A. Yashchenko, A. Lutsyk, I.Tkach |
| Heading |
EXPERIMENTAL MEDICINE |
| Type of article |
Scentific article |
| Annotation |
9 tissue samples of chorionic villi of human embryos, died in the first trimester of pregnancy at 4-13 weeks in-utero were investigated. Two research groups were formed: the first - sporadic miscarriages, the second - recurrent miscarriages. Control group consisted of 5 chorionic villi tissue samples of human embryos obtained after artificial abortion of physiological pregnancies at respective gestational age accomplished due to women’s demand. Histological material was fixed in 4% neutral formalin solution. To study the general morphology the 5-7 µm thick sections were stained with hematoxylin and eosin. Hydrocarbon cell surface glycopolymers determinants of chorionic villi and their intracellular compartments were studied by lectin-peroxidase technique using lectins PNA, GNA, WGA and PFA. Viewing and photographing of histological samples was performed using a microscope “Swift Instruments International”, equipped with camera “Echoo-Imager 502 000” using computer program “TopView 3.2”.
General morphological studies shown changes of the shape and structure of chorionic villi in the investigated samples, compared with the control. Based on lectin histochemical studies the different exhibition level of lectin receptors in the chorionic villi structural components of 4-13-week-old human embryos was observed. The highest expression of lectin receptors WGA, GNA and PFA detected in the study group of recurrent miscarriage, indicating a violation of biopolymers end-stage glycosylation due to excessive activation of glycosidases, compared with a group of sporadic miscarriage and control group.
|
| Tags |
chorionic villi, sporadic miscarriage, recurrent miscarriage, lectin histochemistry, human embryos |
| Bibliography |
- Antonyuk V.O. Lectins and their material sources / V.O. Antonyuk // - 2005. Lviv., 551 p.
- Ajit V. Essentials of Glycobiology, 2nd edition / V. Ajit, Richard D. Cummings, D. Jeffrey [et al.] // Cold Spring Harbor (NY): Cold Spring Harbor Laboratory Press, - 2009.
- Bilyy R. Apoptosis-related changes in plasma membrane glycoconjugates of peripheral blood lymphocytes in rheumatoid arthritis / R. Bilyy, L. Nemesh, V. Antonyuk [et al.] // - Autoimmunity, - 2009, Vol.42, P. 334-336.
- Gabius H. J. The sugar code: fundamentals of glycosciences / H. J. Gabius // John Wiley & Sons, Weinheim, - 2009.
- Hrytsevych N. Search for new markers of endothelial disfunction under nonerosive esophagitis during postprandial hyperglycemia and administration of L-tryptophan / N. Hrytsevych, O. Zayachkivska, A. Yashchenko // Practical Medicine, Lviv, - 2012, Vol.18, P. 29-38.
- Kajii T. "Anatomic and chromosomal anomalies in 639 spontaneous abortuses" / T. Kajii, A. Ferrier, N. Niikawa [et al.] // Human Genetics - 1980, Vol. 55 (1), P.87–98.
- Komath S. S. Organic and Biomolecular Chemistry / S.S. Komath, M. Kavitha, M. J. Swamy // Vol. 4. - 2006. - P. 973–988.
- Katz V. L. Spontaneous and recurrent abortion: etiology, diagnosis, treatment / V. L. Katz, G.M. Lentz, R. A. Lobo [et al.] // Comprehensive Gynecology. 6th ed. Philadelphia, PA: Elsevier Mosby; - 2012.
- Lee R. M. Silver RM 2000 Recurrent pregnancy loss: summary and clinical recommendations / R.M. Lee // - Semin Reprod Med 18, P. 433–440.
- Lutsyk A. D. Lectins in histochemistry / A. D. Lutsyk, E.S. Detyuk, M.D. Lutsyk // - 1989. Lviv: Vyshcha shkola, 144 p.
- Lutsyk A. D. Structural, functional, and lectin histochemical characteristics of rat ovaries and endometrium in experimental hyper- and hypothyroidism / A. D. Lutsyk, E. A. Sogomonian // Folia Histochem Cytobiol, - 2012, Vol 50,P. 331-339.
- Moorghen M. Peanut lectin: a histochemical marker for phaeochromocytomas / M. Moorghen, F. Carpenter // Virchows Arch A Pathol Anat Histopathol, - 1991, Vol. 419, P. 203-207.
- Management of Early Pregnancy Loss. ACOG Practice Bulletin (American College of Obstetricians and Gynecologists) 24 (February). - 2001.
- Mohangoo A. D. "International comparisons of fetal and neonatal mortality rates in high-income countries: should exclusion thresholds be based on birth weight or gestational age?" / A.D. Mohangoo, B. Blondel, M. Gissler [et al.] //- 2013, PLoS ONE Vol.8 (5).
- Nybo Anderson A.M. Maternal age and fetal loss: population based register linkage study / A.M. Nybo Anderson, J. Wohlfahrt, P. Christens [et al.] // BMJ - 2000, Vol.320, P.1708–1712.
- National Coordinating Centre for Women's and Children's Health (UK) (December 2012). “Ectopic Pregnancy and Miscarriage: Diagnosis and Initial Management in Early Pregnancy of Ectopic Pregnancy and Miscarriage”. NICE Clinical Guidelines, No. 154. Royal College of Obstetricians and Gynaecologists. Retrieved 4 July - 2013.
- Rai R. Recurrent miscarriage / R. Rai, L. Regan // Lancet - 2006, Vol.368, P.601-611.
- Roth J. Lectins for histochemical demonstration of glycans / J. Roth // Histochem Cell Biol, - 2011, Vol.136, P. 117-130.
- Royal College of Obstetricians and Gynaecologists (RCOG) (April 2011). “The investigation and treatment of couples with recurrent first-trimester and second-trimester miscarriage”. Green-top Guideline №. 17. Retrieved 2 July - 2013.
- Stephenson M. D. Cytogenetic analysis / M. D. Stephenson, K. A. Awartani, W.P. Robinson
- Stirrat G.M. Recurrent miscarriage; definition and epidemiology / G.M. Stirrat // - Lancet - 1990, Vol.336, P.673–675.
- Susan A. Brooks and Adrian Harris. Breast cancer research protocols / A. Susan // Humana Press Inc. Totowa, New Jersey - 2006.
- Sharon N. Lectins: carbohydrate-specific reagents and biological recognition molecules / N. Sharon // J Biol Chem, - 2007, Vol.282, P. 2753-2764.
- Sokurenko L. M. Application of lectin histochemistry for the evaluation of spinal cord / L. M. Sokurenko, Y. B. Chaikovsky // World Med Biol (Poltava), - 2012, Vol. 2, P. 151-153.
- Yashchenko A. M. Rat liver carbohydrate alterations in streptozotocininduced diabetic rats / A.M. Yashchenko, L.V. Pankevych, A.D. Lutsyk // Eur J Anat, - 2012, Vol.16, P. 82-90.
|
| Publication of the article |
«World of Medicine and Biology» №1(48), 2015 year, 126-132 pages, index UDK 616 |
