| About the author: |
Tokaruk N. S. |
| Heading |
EXPERIMENTAL MEDICINE |
| Type of article |
Scentific article |
| Annotation |
For cluster analysis we used agglomerative hierarchical method and iterative method of k-means. In cellular composition of transitional epithelium four clusters and their morphometric parameters were determined. Cluster 1 takes the smallest share (10.96%), and cell clusters 2, 3 and 4 are respectively 39.47%, 34.21% and 15.35%. Cluster 1 contains the smallest undifferentiated basal cells with high nuclear cytoplasmic ratio (NCR). They act as cambial committed cells that proliferate and regenerate cellular composition of the urothelium. Cluster 4 contains major superficial urothelial cells with the lowest NCR. They are highly differentiated umbrella cells that actually form an urothelial barrier. Clusters 2 and 3 are formed by intermediate layer cells of the transitional epithelium. Cells from cluster 2 are moderately differentiated. They characterize the initial stage of urothelial cells differentiation. Cells from cluster 3 are highly differentiated. They characterize terminal differentiation. It was also determined that the cellular structure of clusters 1 and 4, 2 and 3 are in a particular functional equilibrium. The resulting cluster structure characterizes the urothelial cells population in transitional epithelium of the normal (annual, male) rat bladder. |
| Tags |
cluster analysis, morphometric analysis, transitional epithelium, urinary bladder |
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| Publication of the article |
«World of Medicine and Biology» №1(48), 2015 year, 163-167 pages, index UDK 591.87+591.462+616-092.9+616-074 |
