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    Kondaurova A. Yu.

    ULTRASTRUCTURAL ANALYSIS OF CHIEF CELLS NUCLEI OF RAT GASTRIC MUCOSA AFTER CO-ADMINISTRATION OF ZOLEDRONIC ACID AND HYDROCORTISONE ACETATE


    About the author: Kondaurova A. Yu.
    Heading EXPERIMENTAL MEDICINE
    Type of article Scentific article
    Annotation Studied the effect of zoledronic acid, bisphosphonates relating to the 5th generation, and its combination with hydrocortisone acetate in the different terms of introduction to the structure of the chief cells nuclei of the rats gastric mucosa. Used electron microscopic and morphometric methods of research chief cells of the gastric mucosa. The chief cells were are wrinkled and deformed. The cytoplasm of chief cells vacuolated, slightly stained. Most major cell had enlightened of perinuclear cytoplasm. After 30 days administration of hydrocortisone and zoledronic acid in the nuclei of the chief cells was prevailed heterochromatin, which condensed as near the nuclear membrane and in center of the nucleus. 90 days after the administration of drugs in many major chief cells was observed pronounced widening of perinuclear space. Observed nucleoli fragmentation and rings. With increasing duration of the experiment (90 days) in the nuclei of the chief cells there was tendency to increase the heterochromatin area. Electron microscopic study of the chief cells in the body glands in 30 days after drug administration revealed the predominance of functionally inactive cells. In the nuclei of these gland cells heterochromatin is prevailed, its area is increased by 23% (p <0,05) compared with the control value, euchromatin area decreased by 30% (p <0,05), the thickness of the marginal chromatin increased by 36% (p <0.05). With the increase of the introduction of the zoledronat and hydrocortisone in the nuclei of chief cells trend towards an increase in the heterochromatin area and euchromatin decrease in the area continued to increase the thickness of the marginal chromatin. The predominance of the square of euchromatin area over heterochromatin and increased thickness of chromatin near the nuclear membrane at all stages of administration of hydrocortisone in combination with zoledronic acid, could indicate that the inhibit: uneven distribution of chromatin, its disintegration, exit fragments of nuclear structures and the formation of apoptotic bodies corresponds ultrastructural signs of apoptosis.
    Tags euchromatin, heterochromatin, apoptosis
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    Publication of the article «World of Medicine and Biology» №2(49) 1 part 2015 year, 100-103 pages, index UDK 591.87:591.147