Syrma O. I., Dumbrova N. E., Molchanyuk N. I., Ulyanov V. A., Skobeeva V. M.

COMPARATIVE CHARACTERISTICS OF MORPHOLOGICAL AND FUNCTIONAL CHANGES IN THE RAT SKIN WITH THE INTRODUCTION OF SILVER NANOPARTICLES OF DIFFERENT SIZES

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About the author:	Syrma O. I., Dumbrova N. E., Molchanyuk N. I., Ulyanov V. A., Skobeeva V. M.
Heading	EXPERIMENTAL MEDICINE
Type of article	Scentific article
Annotation	Data about the estimation of size-depended tissue effects of silver nanoparticles (NP) within the framework of one experiment are absent. Also given not enough data about influence on a basal membrane silver NP of different sizes. These null data do not allow estimating the tissue effects of influence NP and those size-depended properties. So, the objective of this work is to study of features of influence of silver NP of different sizes on the rat skin. Methods. Used spherical silver NP 20, 30, 70 nm were synthesized using citrate. The experiment was conducted on 261 Wistar rats. Effect was assessed on 1, 3, 7, 14, 21, 30, 45 days after subcutaneous administration of 0.01 ml solution of silver nanoparticles. For electron microscopic examination of skin fragments were fixed, ultrathin sections were contrasted by the method of Reynolds. Results. As a result of the introduction of silver NP of different sizes edema, separation, fragmentation and partial homogenization of the basement membrane is marked. In this case, the dependence of the NP size of rate of basement membrane recovery was observed. In impact conditions, recovery was observed at day 14 in the group 30 nm silver NP, at day 21 in the group 70 nm, and recovery was not observed until the end of the experiment in the group 20 nm. NP 20 nm decrease the number of young forms of fibroblasts to 60, 1 ± 4,2% (p <0,05); at 30 nm increase of 44,5 ± 3,2% (p <0,05) were marked; 70 nm reversible changes which disappear on day 45 were observed. At 20 nm mitotic activity of basal cells of epidermis is reduced maximum of 29, 3 ± 1, 9% (p <0, 05); at 30 nm mitotic activity is increased of a maximum of 16, 5 ± 2, 2% (p <0, 05) after 14 days; Conclusions: The size-depended effects of silver NP 20, 30, 70 nm are educed on skin, namely dependence on a speeds of proceeding in the structure of basal membrane (quicker than all at introduction of silver NP 30 nm); mitotic activity of basal cells of epidermis (grows among 20≤70≤30); amounts of macrophages (grows among 70≤20≤30) amount of active fibroblasts (grows among 20≤70≤30), that results in the increase of collagen fibers (maximally in the group of 30 nm).
Tags	silver nanoparticles, skin, size-depended effects
Bibliography	Butc T. Proniknovenie nanochastic v dermu: izvestnye i neizvestnye fakty / T. Butc // Kosmetika & medicina. – 2009.–N 5.– S. 42–45. Musina L.A. Rol' makrofagov v regeneracii soedinitel'noj tkani pri implantacii biomaterialov / L.A. Musina, S.A. Muslimov, A.I. Lebedeva // «Aktual'nye voprosy patologii». – Zdravoohranenie Bashkortostana. – 2004. – № 4. – S. 146–149. Mіshalov V.D. Pro pravovі, zakonodavchі ta etichnі normi і vimogi pri vikonannі naukovih doslіdzhen' / V.D. Mіshalov, Ju.B. Chajkovs'kij, І.V. Tverdohlіb // Morfologіja. – 2007 – № 2. – S. 108–115. Sarkisov D. S. Mikroskopicheskaja tehnika: Rukovodstvo / D.S. Sarkisov, Ju.L. Perov // – M.:Medicina, - 1996. – 544 s. Syrma E. I. Morfofunkcional'naja harakteristika dermy kozhi krys pri vvedenii nanochastic serebra / E. I. Syrma, V. M. Skobeeva, V. A. Ul'janov // Vіsnik morfologії. - 2014. - №2. - S. 245-248. Syrma E. I. Razmerozavisimye morfofunkcional'nye izmenenija kozhi krys pri vvedenii nanochastic serebra / E. I. Syrma, V. M. Skobeeva, V. A. Ul'janov // Vіsnik problem bіologії і medicini. - 2014. - №3. - S. 284-287. Arora S. Interactions of silver nanoparticles with primary mouse fibroblasts and liver cells / S. Arora, J. Jain, J. Rajwade //Toxicol Appl Pharmacol. – 2009. – № 236. – Р.310–318. Hubbs A. F. Nanotoxicology-A Pathologist’s Perspective / A.F. Hubbs, R.R. Mercer, S.A. Benkovic [et al.] // Toxicol Pathol. – 2011. – № 2.–Р. 301–324 Kim A. S. Silver nanoparticles induce apoptosis through the Toll–like receptor 2 pathway / A. S. Kim, C. Chae, J. Kim // Oral and maxillofacial pathology. – 2012. – № 6. – P. 789–799. Park J.Size dependent macrophage responses and toxicological effects of Ag nanoparticles / J. Park, D.H. Lim, H.J. Lim [et al.] // Chem Commun. – 2011. – № 47. –P. 4382–4384. Reуnoldes E.S. The use of lead citrate at high pH as an electron–opaque stain in electron microscopy / E.S. Reуnoldes // J. Cell Biol. – 1963. – № 17. – P. 208–212. Samberg M.E. Evaluation of silver nanoparticle toxicity in skin in vivo and keratinocytes in vitro / M.E. Samberg, N.A. Oldenburg, S.J. Monteiro–Riviere // Environ. Health Perspect. – 2010. – №118. – Р. 407–413. Watari F. Material nanosizing effect on living organisms: non–specific, biointeractive, physical size effects/ F.Watari, N.Takashi, A.Yokoyama // J R Soc Interface. – 2009. – №6. – P. 371–388.
Publication of the article	«World of Medicine and Biology» №2(49) 1 part 2015 year, 136-140 pages, index UDK 535.343: 612.017.4