РусскийEnglishУкраїнська
  • Main
  • Useful links
  • Information for Contributors
  • About
  • Editorial board

  • Article
    Kharchenko A. V.

    MICROSATELLITE EXPANSION – MOLECULAR-BIOLOGICAL PHENOMEN OF DIAGNOSIS PRECANCEROUS AND CANCEROUS PROCESSES


    About the author: Kharchenko A. V.
    Heading LITERATURE REVIEWS
    Type of article Review article
    Annotation Relative saturation of genomes with any microsatellite sequences is the result of influence of many factors, which all in all determine composite, structural and thermodynamic features of genomic microsatellite sequences. Polymorphism of microsatellites can be identified by their morphological characteristics. The difference in the degree of polymorphism between various microsatellites may depend first on the length of microsatellite sequence it self. Intensive extension of microsatellite sequences due to replication errors is called microsatellite expansion. The ability of repeats to expansion depends on the length of microsatellite sequence. The relations between mutative events, leading to expansion of microsatellite sequences due to addition of a repeat, correlates with number of mutations, which lead to reduction of repeats in human microsatellites as 10:4. Polymorphism of microsatellites can be identified by their localization and orientation in genome. The secondary structure of DNA is currently viewed as the cause of expansion of microsatellite sequences. The secondary structure of DNA itself is the derivative of thermodynamic characteristics of its sequence. The secondary bulge-type structures in microsatellite sequences, identified by their thermodynamic characteristics, can initiate the phenomenon of expansion of microsatellite repeats. The more stable these bulges, the lower is the risk of formation of new microsatellite repeats. Calculations of thermodynamic characteristics of replicable sequences allow developing number of model systems, evaluating the ability of microsatellite sequences to influence the DNA modifications, forming various secondary structures, related to phenomenon of expansion of microsatellite repeats. Types of markers, obtained as a result of PCR, are divided into two groups on the basis of primers’ design: the first group is known as STSs (sequence-tagged sites) with primers, constructed from known sequences, and the second one is based on the random primers. The most informative or polymorphic STS-marker emerges during amplification of DNA-area, containing sequences of microsatellite repeats. This marker is based on STS, and is marked as simple-sequence length polymorphism (SSLP) or sequence-tagged microsatellite site (STMS). Each STMS-marker detects inherited codominant alleles in single locus in genome.
    Tags microsatellite sequences, microsatellite expansions, replication errors, microsatellite instability, PCR-based markers
    Bibliography
    • Abramov D. D. Tochnost' metoda polimeraznoj cepnoj reakcii «v real'nom vremeni» / D. D. Abramov, D. Ju. Trofimov, D. V. Rebrikov // Prikl. Biohimija i mikrobiologija. – 2006. T.42. S.485 – 488.
    • Markovs'kij V. D. Kompleksna patomorfologіchna diferencіjna dіagnostika peredpuhlinnih procesіv і raku shlunka / V. D. Markovs'kij, O. V. Harchenko // Patologіja. – 2012. – №3. – S. 15–18.
    • Markovs'kij V. D. Vijavlennja disemіnovanih puhlinnih klіtin u periferichnіj krovі u hvorih na virazkovo-іnfіl'trativnij rak shlunka / V. D. Markovs'kij, O. V. Harchenko // Vіsnik morfologії. – 2012. –T.18, №1. – S.135–139.
    • Pat. 76768 Ukraїna A61V 10/00. Sposіb dіagnostiki іnfіl'trativno-virazkovogo raku shlunka / O.V. Harchenko, V.D. Markovs'kij, V.M. Balac'kij. – u 2012 09011; zajavl. 23.07.2012.; opubl. 10.01.2013; Bjul. №1.
    • Rebrikov D. V. PCR «v real'nom vremeni» / D. V. Rebrikov, G. A. Samatov, D. Ju. Trofimov [i dr.] // – M. : BINOM. Laboratorija znanij, - 2009. – 215 s.
    • Baldi P. Sequence analysis by additive scales: DNA structure for sequences and repeats lengths / P. Baldi, P.F. Baisnee // Bioinformatics. – 2000. – Vol. 16. – P. 865 – 889.
    • Bull L. Compound microsatellite repeats: practical and theoretical feautures / L. Bull, C.R. Pabon-Pena., N. B. Freimer // Genome Res. – 2000. – №9 . – P. 830 – 838.
    • Brohede J. Individual variation in microsatellite mutation rate in barn swallows / J. Brohede, A.P. Moller, H. Ellegren // Mutat Res. – 2004. - №12. - Р.73-80.
    • Bordoni A. A microarray platform for parallel detection of five transgenic events in foods: a combined polimerase chain reaction-ligation detection reaction-universal array method / A. Bordoni, A. Germini, A. Mezzelani [et al.] // J Agric Food Chem., - 2005. – Vol. 53 – P. 912 – 918.
    • Cleary J. D. Replication fork dynamics and dynamic mutations: the fork-shift model of repeat instability / J. D. Cleary, C. E. Pearson // Trends Genet. – 2005. – № 21. – Р. 272–280.
    • Cowan C. A. Nuclear reprogrammin of somatic cells after fusion with humen embryonic stem cells / C.A. Cowan // Science. – 2005. – Vol. 309. – P.1369 – 1373.
    • Hartenstine M. J. Base stacking and even/odd behavior of hairpin loops in DNA triplet repeat slippage and expansion with DNA polymerase / M.J. Hartenstine, M.F. Goodman, J. Petruska // J Biol Chem. – 2000. – №24. – Р.18382 – 18390.
    • Hernandez M. Interlaboratory transfer of a PCR multiplex method for simeltaneous detection of four genetically modified maize lines: Bt11, MON810, T25, and GA21 / M. Hernandez, D. Rodrnguez-Lbzaro, D. Zhang [et al.] // Jagric Food Chem. – 2005. Vol.53 – P. 3333 – 3337.
    • Leontis N. B. The non-Watson–Crick base pairs and their associated isostericity matrices / N.B. Leontis, N. Stombaugh, J. Westhof // Nucl.Acid.Res. – 2002. – № 3. – Р. 3497 – 3591.
    • Makova K. D. Evolution of microsatellite alleles in four species of mise Genus apodemus / K.D. Makova, A. Nekrutenko, R.J. Baker // J Mol Evol. – 2000. – № 51. – Р.166 – 172.
    • Scotti I. Microsatellite repeats are not randomly distributed within Norway sprue (Picea abies K.) expressed sequences / I. Scotti, F. Magni, R. Fink [et al.] // Genome. – 2000. – № 4. – Р. 41 – 46.
    • Wren D. J. Repeat polymorphisms within gene regions: phenotypic and evolutionary implication / D. J. Wren, E. Forgacs, J.W. Fondon [et al.] // – 2000. - № 67. – Р.345 – 356.
    • Xu X. The direction of microsatellite mutations is dependent upon allele length / X. Xu, M. Peng, Z. Fang // Nat Genet. – 2000. – Vol.4. – №4. – Р. 396 – 399.
    Publication of the article «World of Medicine and Biology» №2(49) 1 part 2015 year, 195-199 pages, index UDK 616 – 006.6 : 577.2