Shtanyuk YE. A.

INVESTIGATION OF ANTIBACTERIAL ACTIVITY OF OINTMENTS WITH LEVOFLOXACIN AND DECAMETOXINE ON CLINICAL STRAINS - AGENTS OF WOUND INFECTIONS

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About the author:	Shtanyuk YE. A.
Heading	CLINICAL MEDICINE
Type of article	Scentific article
Annotation	The actual problem is medical treatment of surgical wounds complicated by multiresistant strains of bacteria to antibiotics. Resistance of bacterial microflora to antimicrobial agents is increasing every year and requires implementation in medical practice of new effective drugs. According to the literature the main causative agents of inflammatory diseases is Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa. It is advisable to monitor the efficiency of modern antibiotics (for example fluoroquinolones), antiseptics and other excipients that can potentially overcome bacterial resistance to these antibiotics. The purpose of the study. Research by agar diffusion of antibiotic action of ointments levofloxacin 0.1% and ointment decametoxine on standard and clinical strains of S. aureus, E. coli and P. aeruginosa and the search for solutions to overcome the resistance of clinical strains these types of bacteria to levofloxacin. Materials and methods. In the experiment, 10 samples of ointments with levofloxacin 0.1% and / or 0.5% decametoxine were used for water-soluble base, consisting of poloxamer, polyethylenes and propyleneglycol. Furthermore, some additional samples of ointments were containing 0.5% disodium EDTA complexing (DNE), 10% penetration amplifier of dimethylsulfoxide (DMSO) or N-metylpyrolidon (NMP) or transkutol (monoetylovyy ether of diethylene glycol – MED), 0.5% cationic surfactant active substances (SAS) or miramistin etoniy, or 1% anionic surfactant sodium lauryl sulfate (SLS). Antibiotic ointments action tested on standard and nosocomial multiresistant strains of S. aureus, E. coli and P. aeruginosa. Each study was repeated 6 times. Reliability of the results was determined by Student's t test (p<0.05). Results and discussion. The strain of E. coli № 6 was insensitive to ointment of levofloxacin 0.1%. Adding 0.5% DNE significantly contributes to stunted growth areas of the clinical strain of E. coli № 6. Significant (p ≤ 0.05) increase the diameter of the zones of growth retardation is also observed in addition to the ointment 0.5% DNE in combination with penetration enhancers (DMSO, NMP and MED) and SAS. Only one sample of ointment containing levofloxacin 0.1%, 0.5% DNE, 10% DMSO and 0.5% decametoxine, has effective action against strains of P. aeruginosa № 17. In the next series of experiments was investigated samples of ointments with decametoxine and the best sample ointment with levofloxacin in standart and clinical strains of S. aureus, E. coli and P. aeruginosa. Ointments with decametoxine act effectively on all standard and investigational clinical strains of bacteria. Highly sensitive standard strains of bacteria S. aureus, E. coli and P. aeruginosa and clinical strain of S. aureus № 910 were to ointment of levofloxacin 0.1%. Studies indicate that decamethoxin, DMSO and DNE potentsirovat antibacterial action of ointment with levofloxacin 0 1% against the standard and clinical strains of Staphylococcus and E. coli. Clinical strains of P. aeruginosa are resistant to levofloxacin in the form of water-soluble ointment base, and its effectiveness is determined for them mainly decametoxine. Conclusions. Ointment with levofloxacin appears highly relative against standard strains of S. aureus, E. coli and P. aeruginosa and clinical strain of S. aureus, but relatively ineffective and inefficient against clinical strains of gram-negative bacteria of E. coli and P. aeruginosa, respectively. Decametoxine, DMSO and DNE potentsirovat antibacterial action of ointment with levofloxacin 0.1% against the standard and clinical strains of Staphylococcus and E. coli. The effectiveness of water-soluble ointment with levofloxacin 0.1%, 0.5% decametoxine, 10% DMSO and 0.5% DNE is determined mainly decametoxine against clinical strains of P. aeruginosa.
Tags	levofloxacin, decametoxine, ointments, bacteria, antibacterial action, growth retardation zone
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Publication of the article	«World of Medicine and Biology» №3(52) 2 part 2015 year, 074-077 pages, index UDK 579.842.11:579.84:615.454.1:615.33:616-001-092.9