| About the author: |
Ambarova N. A. |
| Heading |
EXPERIMENTAL MEDICINE |
| Type of article |
Scentific article |
| Annotation |
Having used postnatal renal morphogenesis and experimental diabetic nephropathy as test-models in rats we investigated the prospective usefulness of Clitocybe nebularis fungus lectin (CNFA) for histochemical research. It was documented possibilities of this lectin application as a specific histochemical marker of renal corpuscles and of proximal tubules brush border, for morphometric studies, as well as reliable histochemical reagent for the investigation of qualitative rearrangement of renal glycoconjugates under physiological conditions and in histopathology research. Comparison of CNFA with lectins of similar carbohydrate specificities – peanut agglutinin (PNA), soybean agglutinin (SBA), Helix pomatia agglutinin (HPA) – demonstrated certain differences in labeling, encompassing unique histochemical properties of this lectin. |
| Tags |
rat kidney, postnatal morphogenesis, diabetic nephropathy, lectin CNFA |
| Bibliography |
- Antonyuk V.O. Lektini ta yih sirovinni dzherela / V.O. Antonyuk // Lviv: Kvart, - 2005.- S. 328-329.
- Antonyuk R.V. lektinogistohimichne doslidzhennya tovstoyi kishki lyudini v normi ta pri neplastichnih protsesah z vikoristannyam lektiniv, spetsifichnih do T-antigenu ta N-atsetillaktozaminu / R.V. Antonyuk, O.D. Lutsik // Svit meditsini ta biologiyi.-2015.-No. 2(49).-S.73-78.
- Lutsik A.D. Lektinyi v gistohimii / A.D. Lutsik, E.S. Detyuk, M.D. Lutsik // Lviv: Vischa shkola, - 1979.-S.7-37.
- Yaschenko A. M. TsitotopografIya retseptorIv lektinIv u strukturnih komponentah organIv Imunogenezu / A.M. Yaschenko, V.O. Antonyuk, O.V. Nakonechna [ta In.] // - LvIvskiy medichniy chasopis.-2005.-T.9, No. 3.-S. 96-100.
- Dan X. Development and applications of lectins as biological tools in biomedical research / X. Dan, W. Liu, T.B. Ng // Med.Res.Rev.-2015. P. 213-263.
- Gabius H.J. The sugar code. Fundamentals of glycosciences / H.J.Gabius // Weinheim: Wiley, - 2009.-P. 317-328.
- Horejsi V. Studies on lectins. XXXVI. Properties of some lectins prepared by affinity chromatography on O-glycosyl polyacrylamide gels / V. Horejsi, J. Kocourek // Biochim.Biophys.Acta.-1978.- Vol.538, № 2.-P.299-315.
- Lutsyk A. Diabetic alteration versus postnatal maturation of rat kidney glycoconjugates – comparative detection by lectin probes/ A. Lutsyk, N. Ambarova, V. Antonyuk // Folia Histochem.Cytobiol.-2013.- Vol. № 1.-P.92-102.
- Roth J. Lectins for histochemical demonstration of glycans/ J.Roth // Histochem.Cell Biol.-2011.-Vol.136.-P.117-130.
|
| Publication of the article |
«World of Medicine and Biology» №1(55), 2016 year, 119-121 pages, index UDK 616.61-079:[616-018:547.96:582.28]-07 |
