EnglishУкраїнська
  • Main
  • Useful links
  • Information for Contributors
  • About
  • Editorial board

  • Article
    Vorobey E.S., Voronkova O.S., Vinnikov A.I.

    COMPARATIVE CHARACTERISTICS OF MICE VAGINAL DYSBIOS MODELS, CAUSED BY INTRODUCING OF STRAINS OF STAPHYLOCOCCUS AUREUS


    About the author: Vorobey E.S., Voronkova O.S., Vinnikov A.I.
    Heading EXPERIMENTAL MEDICINE
    Type of article Scentific article
    Annotation Comparative analysis of qualitative and quantitative composition of microflora of the mice’s vagina showed that more significant changes were defined under the dysbiosis, caused by filmforming strain of S. aureus (DFS) comparative to dysbiosis caused by non-filmforming strain of S. aureus (DNS). Thus, under the DFS amount of microaerophilic lactobacilli decreased to 1.07±0.58 lg CFU/ml and frequency of detection was 44.1%, while DNS – 1.71±1.10 lg CFU/ml with the frequency of detection 70.1%. The amount of anaerobic lactobacilli in first group decreased to 3.60±2.46 lg CFU/ml and frequency of detection 44.1% and in second group 3.77±2.28 lg CFU/ml at a frequency of 49.3%, respectively. The amount of staphylococci in model with DFS increased to 3.36±2.36 lg CFU/ml, while DNS – to 2.99±1.38 lg CFU/ml with the frequency of detection 100% in both cases, while the amount of Enterobacteriaceae increased to 2.39±1.30 lg CFU/ml with the frequency of detection 19.1% and to 2.00±1.14 lg CFU/ml and the frequency 19.4% respectively. Besides them, an increase of total microbial amount to 2.79×105 CFU/ml for DFS models and to 1.62×105 CFU/ml – for DNS took place, and the ratio of aerobic to anaerobic bacteria were 1:83 and 1:90 respectively. Thus, DFS characterized by a significant increase of the number of opportunistic and anaerobic bacteria and prolonged stability of dysbiosis.
    Tags staphylococcus, biofilm, dysbiosis, reproductive tract
    Bibliography
    • Berezovskaya E.S. Bioplenki pri bakterialnom vaginoze / E.S. Berezovskaya, I.O. Makarov, M.A. Gomberg [i dr.] // Akusherstvo, ginekologiya i reproduktsiya. – 2013. – T. 7, No. 2. – S. 34-36.
    • Bondarenko V.M. Vaginalnaya mikroekosistema v norme i patologii / V.M. Bondarenko, K.R. Bondarenko // Byulleten Orenburgskogo nauchnogo tsentra UrO RAN. – 2014. – No. 1. – S. 1-12. – Rezhim dostupa k zhurnalu: http: //www.elmag.uran.ru     
    • Voronkova O.S. Vpliv antibiotikiv ta tsitostatikiv na formuvannya disbakteriozu pihvi: avtoref. dis. … kand. biol. nauk: 03.00.07 / O.S. Voronkova; In-t mikrobiologiyi i virusologiyi im. D.K.Zabolotnogo NAN Ukrayini. – K.,2010.–23 s.
    • Danshina A.V. Mikroekologicheskie osobennosti komponentov bioplenki reproduktivnogo trakta zhenschin: avtoref. dis. … kand. biol. nauk: 03.02.08 / A.V. Danshina; FGBOU VPO «Ulyanovskiy gosudarstvennyiy universitet». – Ulyanovsk, 2012. – 22 s.
    • Dobrohotova Yu.E. Sovremennyie predstavleniya o mehanizmah razvitiya disbioza vlagalischa / Yu.E. Dobrohotova, N.G. Zatikyan // Orzhin. Akusherstvo, ginekologiya i reproduktsiya. – 2008. – No. 1. – S. 7-9.
    • Yevseev A.A. Vaginalnyiy disbioz i metodyi ego korrektsii / A.A. Evseev // Rossiyskiy vestnik akushera-ginekologa. – 2007. – No. 4. – S. 65-69.
    • Yevropeyska konventsiya pro zahist hrebetnih tvarin, scho vikoristovuyutsya dlya doslidnih ta inshih naukovih tsiley / Strasburg, 18 bereznya 1986 roku: zbIrka dogovoriv Radi Evropi: ukrayinska versiya // [za red. E.M. Vishnevskogo ]. – K.: Parlamentske vidavnitstvo, 2000. – 654 s.
    • Kira E.F. Nespetsificheskiy vaginit i ego vliyanie na reproduktivnoe zdorove zhenschinyi (obzor literaturyi) / E.F. Kira, S.Z. Muslimova // Problemyi reproduktsii. – 2008. – No. 5. – S. 8-14.
    • Mayanskiy A.N. Stafilokokkovyie bioplenki: struktura, regulyatsiya, ottorzhenie / A.N. Mayanskiy, I.V. Chebotar // Zhurnal mikrobiologii, epidemiologii i immunobiologii. – 2011. – No.1. – S. 101-108.
    • Ob unifikatsii mikrobiologicheskih (bakteriologicheskih) metodov issledovaniya, primenyaemyih v kliniko-diagnosticheskih laboratoriyah lechebno-profilakticheskih uchrezhdeniy: prikaz No. 535. – [chinniy vId 22.04.1985 r.]. – M.: MOZ SSSR, 1985. – 65 s.
    • Opredelitel bakteriy Berdzhi: [rukovodstvo] / [pod. red. Dzh. Houlta, N. Kriga, P. Snita [i dr.], per. s angl.]: v 2-tt. – M.: Mir, 1997. – T. 1. – 1997. – 432 s.
    • Opredelitel bakteriy Berdzhi: [rukovodstvo] / [pod. red. Dzh. Houlta, N. Kriga, P. Snita [i dr.], per. s angl.]: v 2-tt. – M.: Mir, 1997. – T. 2. – 1997. – 368 s.
    • Ordiyants I.M. Bakterialnyiy vaginoz: diagnostika i lechenie na sovremennom etape / I.M. Ordiyants, E.S. Chetvertakova, A.A. Chyimba [i dr.] // Zemskiyvrach. – 2011. – No. 2. – S. 28-30.
    • Pro zahist tvarin vId zhorstokogo povodzhennya: zakon Ukrayini vid 21 lyutogo 2006 r. No.3447-IV // Vidomosti Verhovnoyi Radi Ukrayini. – 2006. – No.27. – S. 230.
    • Radzinskiy V.E. Terapiya vaginalnyih infektsiy: grani problemyi (mezhdunarodnyie realii i rossiyskiy opyit) / V.E. Radzinskiy, M.B. Hamoshina, L.A. Shelenina [i dr.] // Doktor. Ru. – 2013. – No. 7 (85). – S. 13-17.
    • Hamoshina M.B. Narusheniya mikrobiotsenoza urogenitalnogo trakta: grani problemyi, perspektivyi korrektsii i profilaktiki / M.B. Hamoshina, V.E. Radzinskiy, A.S. Kalendzhyan [i dr.] // Voprosyi ginekologii, akusherstva i perinatologii. – 2009. – T. 8, No. 5. – S. 69-74.
    • Schuruk N.V. Korektsiya disbiotichnih staniv statevih shlyahiv u vagitnih iz nevinoshuvannyam shlyahom dvoetapnogo pidhodu do likuvannya / N.V. Schuruk // Bukovinskiy medichniy visnik. – 2014. – T. 18, No. 2 (70). – S. 127-131.
    • Swidsinski A. Presenceof a polymicrobial endometrial biofilm in patients with bacterial vaginosis / A. Swidsinski, H. Verstraelen, V. Loening-Baucke [etal.] // PLoSOne. – 2013. – Vol. 8, № 1. – P. 1-5. – Modeofaccess: www.plosone.org.
    • Verstraelen H. The biofilm in bacterial vaginosis: implications for epidemiology, diagnosis and treatment / H. Verstraelen, A. Swidsinski // Current Opinion in Infectious Diseases. – 2013. – Vol. 26, № 1. – P. 86-89.
    Publication of the article «World of Medicine and Biology» №2(56), 2016 year, 110-115 pages, index UDK 579.61:616-078