EnglishУкраїнська
  • Main
  • Useful links
  • Information for Contributors
  • About
  • Editorial board

  • Article
    Chereshnyuk I.L., Povkh V.L., Zagorii G.V., Khodakovskyi A.A., Ostra E.A.

    USAGE OF NEUROMARKER (S100 PROTEIN) AND FLOW CYTOMETRY FOR COMPARATIVE ASSESSMENT OF NMDA-RECEPTOR ANTAGONISTS' EFFECT ON NEUROPROLIFERATIVE PROCESSES IN RETINA AND OPTIC NERVE IN CASE OF MODELLED OPHTHALMIC ISCHEMIA-REPERFUSION


    About the author: Chereshnyuk I.L., Povkh V.L., Zagorii G.V., Khodakovskyi A.A., Ostra E.A.
    Heading EXPERIMENTAL MEDICINE
    Type of article Scentific article
    Annotation The purpose of the study is to investigate the changes of the proliferative processes cells in retina and optic nerve during postreperfusional period of acute ocular ischemia using the flow cytometric analysis and measurement of neuromarker activity, and to assess the efficacy of NMDA receptor antagonists in case of mentioned pathology. Such investigation aims not only the implementation of the new methods for pre-clinical research of drugs with neurotropic vector of pharmacological activity, but allows assessment of the possibility for already known drugs used in neurological practice to be applied for the new indication, particularly as neuroretinoprotectors. In the course of research we determined the significant increase of percentage ratio of the cells in DNA synthetic phase (phase S) to their total amount in the tissue suspension for a median of 7,46 times in the modelled 60 minute ophthalmic ischemia-reperfusion at the end of the first day of observation. This is followed by the increase of neuroglial proliferation marker (S100 protein) titer for a median of 32,6 times (7th day). Comparative assessment of the NMDA receptor antagonists' effect on neuroproliferative processes in retina and optic nerve in case of modelled ophthalmic ischemia-reperfusion using the change of S100 protein titer as neuromarker, together with the flow cytometry method, showed that the most significant effect on the mentioned processes was expressed by ademol. The data of enzymatic test show that this drug has significant advantage of memantine and magnesium sulfate solution, and correlates with the last mentioned by the cytological index of proliferative activity (intensification of S-phase of cell cycle). Іt is reasonable and rational to use the proposed methods and markers for the assessment of the value and grade of ischemic injury retina and optic nerve, and the investigation of NMDA receptor antagonists is prospective for clinical assessment using the new indication - as neuroretinoprotectors.
    Tags ademol, memantine, magnesium sulfate, NMDA receptor antagonists, neuroretinoprotection
    Bibliography
    • Belenichev I. F. Ratsionalnaya neyroprotektsiya / I. F. Belenichev, V. I. Cherniy, Yu. M. Kolesnik [i dr.] // – Donetsk: Izdatel Zaslavskiy A.Yu., – 2009. – 262 s.
    • Bagauri O. V. Eksperimentalna otsinka vplivu pohidnogo 3,2'-spiro-pirrolo-2-oksindolu (spoluka r-86) na dinamiku protsesiv neyrodestruktsiyi pri gostriy tserebralniy ishemiyi / O. V. Bagauri // Ukrayinskiy visnik psihonevrologiyi. – 2014. – No. 2 (79). – S. 22–25.
    • Serdyuk V. M. Kliniko-eksperimentalne obgruntuvannya neyroprotektsiyi v kompleksi likuvannya hvorih na pervinnu vidkritokutovu glaukomu: avtoref. dis. na zdobuttya nauk. stupenya doktora med. nauk / V. M. Serdyuk – Odesa., - 2015. – 36 s.
    • Hodakivskiy O. A. porivnyalna otsinka vplivu pohidnih adamantanu spoluk YuK-1 ta YuK-4 na aktivnIst NMDA-retseptoriv / O. A. Hodakivskiy // Klinichna farmatsiya. – 2011. – T. 15, No. 4. – S. 60-63.
    • Hodakivskiy O. A. Doslidzhennya vplivu pohidnogo adamantanu ademolu na fragmentatsiyu DNK yader neyroniv lobnih chastok kori za ishemiyi-reperfuziyi golovnogo mozku u schuriv / O. A. Hodakivskiy, I. L. Chereshnyuk // Ukrayinskiy visnik psihonevrologiyi. – 2013. – T. 21, vip. 1(74). – S. 26-28.
    • Hodakivskiy O.A. Harakteristika protiIshemIchnih ta mnemotropnih vlastivostey ademolu pri modelnomu gostromu porushenni mozkovogo krovoobigu / O. A. Hodakivskiy // Fiziologichniy zhurnal. – 2013, T. 59, No. 5. – S. 71-77.
    • Hodakivskiy O. A. Patogenetichne obgruntuvannya dotsilnosti vikoristannya novih pohidnih adamantanu pri eksperimentalniy terapiyi gostroyi ishemiyi golovnogo mozku ta miokarda: avtoref. dis. na zdobuttya nauk. stupenya doktora med. nauk / O. A. Hodakivskiy. – Odesa., - 2014. – 32 s.
    • Chereshnyuk I. L. Zastosuvannya protochnoyi tsitometriyi dlya skriningovoyi otsinki vmistu DNK v yadrah klitin neyronalnoyi sitkivki v schuriv / I. L. Chereshnyuk // Visnik morfologiyi. – 2015. T. 21, No. 1 – S.222-226.
    • Belda X. Acute stress induced sensitization of the pituitary–adrenal response to heterotypic stressors: Independence of glucocorticoid release and activation of CRH1 receptors / X. Belda, N. Daviu, R. Nadal [et al.] // Hormones and Behavior. – 2012. – Vol. 62, № 4. – Р. 515-524.
    • Osborne N. N. Topically applied betaxolol attenuates NMDA-induced toxicity to ganglion cells and the effects of ischemia to the retina / N. N. Osborne, L. DeSantis, J. H. Bae [et al.] // Exp. Eye Res. – 1999. – Vol. 69, № 3. – Р. 331-342.
    • Simone F. Biotechnology, animal health and animal welf are within the framework of European Unionlegislation / F. Simone; J. Serratosa // Rev. Sci. Tech. Оіе. – 2005. – Vol. 24, №.1 – Р. 89-99.
    Publication of the article «World of Medicine and Biology» №2(56), 2016 year, 159-164 pages, index UDK 617.21: 617.735: 617.731: 617.7-005.4: 576.35