| About the author: |
Shatalin B.O., Kostenko V.O. |
| Heading |
EXPERIMENTAL MEDICINE |
| Type of article |
Scentific article |
| Annotation |
This experiment involved 28 white rats was designed to study the effect of nuclear factor κB (NF-κB) inhibitor – JSH-23 (4-methyl-N- (3-phenylpropyl) benzene-1,2-diamine) – on quantitative and qualitative indicators of sperm under the conditions combining chronic intoxication with sodium nitrate (200 mg / kg for 30 days) and exposure to X-rays (in a dose of 0.08 Gy three times a week on alternate days, for a total of 0.25 Gy). It has been shown the administration of JSH-23 (1 mg/kg, twice a week) in experimental conditions significantly reduces the percentage of non-viable cells and their pathological forms (with caput abnormalities). At the same time the motility of spermatozoa becomes impaired that is reflected in the cell increase with the rapid forward movement (normokinesis, category A), as well as sperm cells with oscillating disordered movement (dyskinesis, category C), reducing the cell with a slow forward movement (hypokinesis, category B) and fixed sperm (akinesis, category D). |
| Tags |
nuclear factor kappa B, nitrate intoxication, X-ray irradiation, spermiogram, kinesiogram |
| Bibliography |
- Bazarnova M. A. Rukovodstvo k prakticheskim zanyatiyam po klinicheskoy laboratornoy diagnostike / M. A. Bazarnova, V.T. Morozova // – K.: Vischa shkola, - 1988. – 318 s.
- Barilyak I. R. Vivchennya gonadotoksichnoyi diyi novih likarskih zasobiv ta yih vplivu na reproduktivnu funktsiyu tvarin / I.R. Barilyak, L.V. Neumerzhitska, T.F. Bishovets [ta in.] // – K. : Avitsena, - 2001. – S. 139-152.
- Dolgov V. V. Laboratornaya diagnostika muzhskogo besplodiya / V.V. Dolgov, S.A. Lugovskaya, N.D. Fanchenko [i dr.] // – M. – Tver: Triada, -2006. – 145 s.
- Shatalin B. O. Sostoyanie okislitelnogo metabolizma semennikova na fone deystviya nitratnoy intoksikatsii i rentgenovskogo oblucheniya / B. O. Shatalin, V. A. Kostenko // Aktualnyie problemyi transportnoy meditsinyi. – 2014. – No. 3. – S. 56-61.
- Shatalin B. O. Pokazniki funktsionalnogo stanu spermi bilih schuriv za umov sukupnoyi diyi na organizm nitratu natriyu ta rentgenivskogo oprominennya / B. O. Shatalin, V. O. Kostenko // Aktualni problemi suchasnoyi meditsini: Visn. Ukrayinskoyimed. stomatol. akademiyi. – 2016. – T.16, – No.3 – C. 192-195.
- Delfino F. Stage-specific nuclear expression of NF-kappa B in mammalian testis / F. Delfino, W.H. Walker // Mol. Endocrinol. – 1998. – Vol.12, №11. – P. 1696-1707.
- Hayden M. S. Shared principles in NF-kappaB signaling / M. S. Hayden, S. Ghosh // Cell. – 2008. – Vol. 132, № 3. – P. 344-362.
- Kumar A. JSH-23 targets nuclear factor-kappa B and reverses various deficits in experimental diabetic neuropathy: effect on neuroinflammation and antioxidant defence / A. Kumar, G. Negi, S.S. Sharma // Diabetes Obes. Metab. – 2011. – Vol. 13, № 8. – P.750-758.
- Lysiak J. J. Activation of the nuclear factor kappa B pathway following ischemia-reperfusion of the murine testis / J.J. Lysiak, H.J. Bang, Q.A. Nguyen, T.T. Turner // J. Androl. – 2005. – Vol.26. – P. 129-135.
- Rasoulpour R. J. NF-kappa B activation elicited by ionizing radiation is proapoptotic in testis / R. J. Rasoulpour, K. Boekelheide // Biol. Reprod. – 2007. – Vol.76, №2. – P. 279-285.
- Vos T.A. Expression of inducible nitric oxide synthase in endotoxemic rat hepatocytes is dependent on the cellular glutathione status / T.A. Vos, H. Van Goor, L. Tuyt [et al.] // Hepatology. – 1999. – Vol. 29, № 2. – P. 421-426.
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| Publication of the article |
«World of Medicine and Biology» №3(57), 2016 year, 158-161 pages, index UDK 612.616-092.9:615.916’175:616-073.7 |
