| About the author: |
Paltov Ye.V., Chelpanova I.V., Fik V.B., Vil’hova I. V., Kyryk Kh. A., Kryvko Yu. Ya. |
| Heading |
EXPERIMENTAL MEDICINE |
| Type of article |
Scentific article |
| Annotation |
The objective set in the given study wasto examine pathomorphological manifestations of angio-and neuroretinopathy as a result of chronic exposure to therapeutic doses of opioid analgesics.
Sexually mature outbred male rats in the amount of 15 animals weighing 190 grams, age 4.5 months served as study materials. Animals were injected intramuscularly with nalbuphine daily once a day in the same period of time (10-11 am) for 42 days. The initial dose of nalbuphine during the first 2 weeks was 0.212 mg/kg, the next 2 weeks (II - IV week) 0.225 mg/kg, and during IV- VI weeks - 0.252 mg/kg. Thus, chronic opioid effect was obtained [20].
Animals were divided into 2 groups. Group 1 animals received nalbuphine for 42 days, followed by sampling of study material (end of 6th week of experimental opioid effect); Group 2 of controls that was injected with normal saline intravenously for 42 days in the same period of time (10 - 11 am). All animals were in vivarium and conditions that dealt with issues of maintenance, care, labelling and all other manipulations were carried out in compliance with the provisions of “European Convention for the Protection of vertebrate animals used for experimental and other scientific purposes” [Strasbourg, 1985], “General ethical principles of animal experimentation” adopted by the first National Congress on Bioethics [Kyiv, 2001], the Law of Ukraine No3447 – IV “On prevention of cruelty to animals”. Bioethics Committee of Danylo HalytskyLviv National Medical University determined that the given study met the ethical requirements according to MOH of Ukraine No 231 of 01.11.2000 (Record No 10 of 26.12.2011), (record No 2 of 20.02.2012). Before sampling of material vital animals were narcotized by intraperitoneal administration of sodium thiopental (calculated as 25 mg/kg). The rats’ eyeballs were used as the material for microstructural research obtained by postvital enucleation with further actions to preserve topographic relationship of eye membranes. Histological sections 5 - 7 microns thick were produced. Histologic specimens were prepared by the conventional method dyed with hematoxylin, eosin and azane by Heidenhain. Microscopic examination and photographic recording of specimens were carried out by means of a microscope MBI-1 and digital camera Nikon D3100.
Results of the study in the future will allow us to form pathomorphological database that can be used to conduct comparative characteristics. Concerning the processes of the increase of pathological changes in the layers of retina during short-term and long-term effects of small doses of opioids, the above-mentioned will enable further development of methods for optimal correction of angio- and neuroretinopathy caused by opioid effects.
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| Tags |
opioid, retina, rat |
| Bibliography |
- Adrashkin A. P. Rol serdechno – sosudistoy sistemyi v tanatogeneze u opiynyih narkomanov vtoroy stadii /A. P. Adrashkin, I. V. Salomatin, B.F. Murashov [i dr.] – М.,– 2003.– 18 s.
- Vilkhova I. V. Morfolohichni zminy nyrkovoho tiltsia pry dvo–, chotyry– ta shestytyzhnevomu vplyvi nalbufinu (eksperymentalne doslidzhennia) / I. V. Vilkhova // Ukrainskyi medychnyi almanakh. – 2014. – Tom 12, No.1. S.13 –16.
- Vilkhova I. V. Zminy struktury nyrkovoho tiltsia na riznykh terminakh khronichnoho opioidnoho vplyvu/ I. V. Vilkhova // Svit medytsyny ta biolohii. – 2014. – Tom. 46, No.4. – S. 78 – 81.
- Vilkhova I. V. Patomorfolohichni zminy nyrkovoho tiltsia na piznikh terminakh khronichnoho opioidnoho vplyvu) / I.V. Vilkhova // Zhurnal klinichnykh ta eksperymentalnykh medychnykh doslidzhen. – 2015. – Tom. 3, No. 1. – S 25 – 31.
- Vilkhova I. V. Morfolohichni zminy kanaltsiv nefrona pry khronichnomu opioidnomu vplyvi / I. V. Vilkhova // Svit medytsyny ta biolohii. – 2015. – Tom. 49, No.2. – S. 85 – 88.
- Lohash M. V. Deiaki aspekty istorii opiativ v konteksti suchasnykh problem narkozalezhnosti / M. V. Lohash, P. B. Pokotylo // Visnyk problem biolohii ta medytsyny. – 2013. – Vyp. 4, T. 1.(42) – S. 129 – 131.
- Onysko R. M. Mikrostrukturna orhanizatsiia tkanyn yazyka na 28 dobu pislia vidminy opioida / R. M. Onysko, I. O. Onysko // Novyny stomatolohii. – 2013. – No. 3 (76). – S. 101 – 106.
- Onysko I.O. Zminy na elektronnnomikroskopichnomu rivni v tkanynakh yazyka pid vplyvom malykh doz opioidu v kintsi 6 i 8 tyzhniv (eksperymentalne doslidzhennia) / I. O. Onysko, A. P. Korol, O. Ye. Maievskyi [ta in.] // Biomedical and Biosocial Antropology. – 2013. − No.2. – S. 13 – 19.
- Popyk P. M. Morfometrychna kharakterystyka zmin lanok hemomikrotsyrkuliatornoho rusla pidshlunkovoi zalozy pid vplyvom nalbufinu / P.M. Popyk / Visnyk Ukrainskoi medychnoi stomatolohichnoi akademii. –2013. – T.13, Vypusk 4. – S. 158 –161.
- Pidvalna U.Ye. Morfometrychna kharakterystyka perebudovy sudynnoi obolonky ochnoho yabluka pid vplyvom nalbufinu / U.Ye. Pidvalna // Ukrainskyi zhurnal Klinichnoi ta laboratornoi medytsyny. – m. Luhansk, 2013. – T.8, No.3. – S. 94–97.
- Patent No.76565 Ukraina. Sposib modeliuvannia khronichnoho opioidnoho vplyvu ̸ Ye.V. Paltov, V.B. Fik, I.V. Vilkhova, R.M. Onysko, O.S. Fitkalo, Yu. Ya. Kryvko; zaiavnyk i patentovlasnyk Lvivskyi natsionalnyi medychnyi universytet imeni Danyla Halytskoho. – opubl. 10. 01. 2013, Biul. No.1.
- Romeys B. Mikroskopicheskaya tehnika / B. Romeys // – M.: Meditsina, - 1953. – S. 71 – 72.
- Raietska L. V. Tendentsii poshyrennia narkomanii v Ukraini /L.V. Raietska // Borotba z orhanizovanoiu zlochynnistiu i koruptsiieiu.– 2008.– S. 318.
- Treschinskiy I. S. Nekotoryie voprosyi narkomanii i toksikomanii na Ukraine / I .S. Treschinskiy, L. A. Harchenko, V .A. Usenko // Provizor. – 1998. – No. 4 . – S. 15 – 17.
- Yakymiv N. Ya. Toksychne urazhennia zorovykh nerviv vnaslidok tryvaloho pryiomu levomitsetynu na tli narkotychnoi zalezhnosti vid kodterpinu / I. Ya. Novytskyi, N. Ya. Yakymiv, O. M. Yerokhova [ta in.] // Oftalmol. zhurnal. – 2012. – No. 3. – S. 43–45.
- Yakymiv N. Ya. Mikrostrukturna kharakterystyka raiduzhno-rohivkovoho kuta ochnoho yabluka shchuriv pry opioidnomu vplyvi / N. Ya. Yakymiv, Yu. Ya. Kryvko // Svit medytsyny ta biolohii. – 2013. – No. 4. – S. 120–124.
- Yakymiv N. Ya. Ultrastrukturna kharakterystyka struktur raiduzhno-rohivkovoho kuta ochnoho yabluka shchuriv na 35-u ta 42-u dobu opioidnoho vplyvu / N. Ya. Yakymiv // Svit medytsyny ta biolohii. – 2014. – No. 2. – S. 185–188.
- Yakymiv N. Ya. Ultrastrukturna kharakterystyka struktur raiduzhno-rohivkovoho kuta ochnoho yabluka shchuriv na 7-u, 14-u, 21-u, 28-u dobu opioidnoho vplyvu / N. Ya. Yakymiv // Ukrainskyi morfolohichni almanakh. – 2014.No. 2. S. 28–31.
- Yakimiv N. Ya. Morfologicheskaya harakteristika struktur raduzhno-rogovichnogo ugla kryis na raznyih srokah deystviya i na rannih srokah posle otmenyi eksperimentalnogo opioidnogo vliyaniya / N. Ya. Yakimiv // Oftalmologiya. Vostochnaya Evropa. – 2014. – No. 2. – S. 89–97.
- Paltov Y. Dynamics of the onset of pathological changes in the retinal layers at the end of the first week of opioid exposure / Y. Paltov, Y. Kryvko, V. Fik, I. Vilkhova, Kh. Ivasivka, M. Pankiv, K. Voitsenko // Deutscher Wissenschaftsherold. German Science Herald. – 2016. – No.2. – P. 30 – 33.
- Paltov Y. Pathomorphological manifestations in the retina layers during one - week of opioid analgesic exposure / Y. Paltov, Y. Kryvko, V. Fik, I. Vilkhova, Kh. Ivasivka, M. Pankiv, K. Voitsenko // Natural Science Readings abstracts booc (May 19 – 22, - 2016, Bratislava) . – P. 25 – 27.
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| Publication of the article |
«World of Medicine and Biology» №2(60), 2017 year, 146-150 pages, index UDK 611. 843. 1: 615. 212. 7 ] “ 43 = 06 ” |
