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    Stepansky D. A., Kremenchuk G. N., Koshevaya I. P.

    INFLUENCE OF AEROSOCCUS VIRIDANS AUTO-SIMBIONTS ON CLOSTRIDIUM PERFRINGENS, HAVING IN THE INTESTINE OF THE MICE


    About the author: Stepansky D. A., Kremenchuk G. N., Koshevaya I. P.
    Heading EXPERIMENTAL MEDICINE
    Type of article Scentific article
    Annotation The toxin produced by Clostridium perfringens is an important virulence factor, resulting in severe poisoning in humans and animals. Microorganisms of Aerococcus viridans species are effective for the prevention and treatment of inflammatory diseases caused by pathogenic and opportunistic pathogenic microorganisms. Due to the increasing popularity of personalized treatment by auto-strains of probiotic bacteria, antagonistic properties of autosymbiont Aerococcus viridans towards Clostridium perfringens in mice are of interest. Antagonistic properties of autosymbiont Aerococcus viridans towards Clostridium perfringens, which naturally live in the gut of mice, were investigated in this work. 50 white mice daily for 10 days received per os 0,5 billion of A. viridans 5m2015 in physiological sodium chloride solution. The other 50 white mice daily for 10 days received per os 0,5 billion of A. viridans 167 in physiological sodium chloride solution. Before, during and after 7-10 days of feeding animals by Aerococci bacteriological examination of faeces for the presence and quantity of C. perfringens was made. Before feeding by aerococci in the majority of animals in both groups (100 and 98%), S. perfringens was detected while sowing the initial suspension of feces. At the stool suspension dilution, C. perfringens detection gradually decreased, and was approximately the same in both groups. During feeding expressed antagonistic action of A.viridans 5m2015 and A.viridans 167 and towards the specified type of clostridia in the intestines of mice was noted, as evidenced by the sharp decline in their sowing. After the cessation of feeding, allocation of C. perfringens gradually increased, but did not reach the initial level (before feeding by aerococci) even after 10 days in any of groups. When comparing the antagonistic action of A. viridans 5m2015 and A.viridans and 167, it should be noted that autosymbiont A. viridans 5m2015 had higher antagonism towards C. perfringens that live in the intestines of mice, than 167 museum A. viridans. Conclusions 1. Autosymbionts A. viridans are natural inhabitants of warm-blooded animals unsterile cavities. 2. Autosymbiont strain of A. viridans 5m2015, isolated from feces of mice, has antagonistic effect against C. perfringens, living in the intestine of mice. 3. Autosymbiont strain of A. viridans 5m2015 has higher antagonistic properties to C. perfringens, which lives in the intestine of mice, than strain of museum A. viridans 167. 4. Application of autosymbiont microbes-antagonists may be perspective in prophylaxis and treatment of conditions, caused by C. perfringens, which requires further research.
    Tags Aerococcus viridans, antagonism, autosymbionts
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    Publication of the article «World of Medicine and Biology» №2(60), 2017 year, 161-164 pages, index UDK 579.22:579.262:616.3–008.8–092.9