English Українська
  • Main
  • Useful links
  • Information for Contributors
  • About
  • Editorial board

  • Article
    Chugai O., Lyubinets L.

    PHAGOCYTIC ACTIVITY OF LEUKOCYTES IN THE DYNAMIC OF EXPERIMENTAL PNEUMONIA


    About the author: Chugai O., Lyubinets L.
    Heading EXPERIMENTAL MEDICINE
    Type of article Scentific article
    Annotation Introduction. Respiratory system - is one of the most complex multifunctional systems in the human body. The lungs provide not only the exchange of gases, but also involved in many physiological processes of the body, including the immune defense. Through constant contact with the air space of the environment and enhanced vascularization, the lungs accounts for a significant antigenic load, including various infectious agents. The aim of the study- study phagocytic activity of leukocytes in the dynamics of experimental pneumonia (EP). Materials and methods. Experimental studies conducted on 9 guinea pigs (mammals) weighing 180-220 grams, divided into 4 groups: І group - intact guinea pigs, 3 animals (control); ІІ group - the guinea pigs with EP on the 6th day, 2 animals; III group - the guinea pigs wit EP on the 10th day, 2 animals; IV group - guinea pigs with EP on the 20 th day, 2 animals. The experimental pneumonia reproduced by intranasal administration to animals Staphylococcus aureus culture by the method of Shlyapnykova V.N. et al. [10]. The animals were decapitated on the 6th, 10th and 20th day of EP. Phagocytic activity of monocytes analyzed (PAM) by the cup method. As a test - object phagocytosis used daily live culture of S. aureus (strain 505). When setting PAM determined the following parameters: phagocytic index (PI) - the percentage of phagocytic monocytes, phagocytic number (PN) - the amount of absorbed bacterial cells per 1 monocytes. Also performed spontaneous NBT test in blood by the method M.E. Viksman, A.N. Mayanskoho. The results are statistically evaluated by Student t-test. Results and discussion. As a result of the research found that in guinea pigs with EP observed growth rates of macrophage phagocytic system. Is revealed an increase of PN on the 6th, 10th and 20th day of experiment on 9.8%, 41.1% and 46%, respectively, compared to the control group. In addition, PI also grew respectively on 11.9%, 15.6% and 22.9% on the 6th, 10th and 20th day of study, compared to the control group. With such results by NBT-test, which also grew respectively on 9.0%, 9.8% and 57.8% at on the 6th, 10th and 20th day. Attention is drawn to the fact that despite the uniform growth to PI during the period of formation of pneumonia, the other two indicators showed certain periods of maximum growth. Thus, PN has increased in 1.4 times in just 10 days of the experiment, which was significantly higher compared with the control group, p <0.05. However, NBT test that shows the metabolic activity of lymphocytes digesting 1.6 times increased significantly at 20th day compared with the control group of animals, p <0.05. Conclusion. The course of experimental pneumonia characterized by activation of macrophage phagocytic system manifested in growing blood indicators such as PI, PN, NBT. Amid the trend towards a uniform an increase absorptive capacity of phagocytic lymphocytes, the average number of absorbed infectious agents and digesting activity of monocytes significantly increased at 10 and 20 days of experimental pneumonia.
    Tags experimental pneumonia, phagocytosis, mononuclear phagocytes
    Bibliography
    • Viksman M. E. Sposob otsenki funktsionalnoy aktivnosti neytrofilov cheloveka po reaktsii vosstanovleniya nitrosinego tetrazoliya: Metod. rekomendatsii. / M. E. Viksman, A. N. Mayanskiy. – Kazan: Kazanskiy NIIEM, - 1979. – 11 s.
    • Shlyapnikov V. I. Eksperimentalnyie modeli ostryih pnevmoniy, vyizvannyih uslovno-patologicheskimi bakteriyami i ih assotsiatsiey: metod. ukazaniya / [V. I. Shlyapnikov, T. L. Solodova, S. A. Stepanov i dr.]. – Saratov, 1988. – 30 s.
    • Frolov V. M. Opredelenie fagotsitarnoy aktivnosti monotsitov perifericheskoy krovi u bolnyih / V.M. Frolov, N.A. Peresadin, I.Ya. Pshenichnyiy // Laboratornoe delo. – 1990. – No. 9. – S. 27-29.
    • Aderem A. Mechanisms of phagocytosis in macrophages / A. Aderem, D. M. Underhill. // Annu Rev Immunol. – 1999. – No.17. – P. 593–623.
    • Hampton M. B. Inside the neutrophil phagosome: oxidants, myeloperoxidase, and bacterial killing / M. B. Hampton, A. J. Kettle, C. C. Winterbourn. // Blood. – 1998. – No.92. – С. 3007–3017.
    • Gordon S. Alternative activation of macrophages / S. Gordon. // Nat Rev Immunol. – 2003. – No.3. – P. 23–25.
    • MacMicking J. Nitric oxide and macrophage function / J. MacMicking, Q. W. Xie, C. Nathan. // Annu Rev Immunol. – 1997. – No.15. – P. 323–350.
    • Nelson S. Pathophysiology of pneumonia / S.Nelson, C. M. Mason, J. Knolls [et al.] // Clin. Chest Med. – 1995. – No.16. – P. 1–12.
    • Onofrio J. M. Granulocyte-alveolar macrophage interaction in the pulmonary clearance of Staphylococcu.s aureus / J. M. Onofrio, G. B. Toews, M. F. Lipscomb. // Am. Rev. Respir. Dis. – 1983. – No.127. – P. 335–342.
    • Steinman R. M. Endocytosis and the recycling of plasma membrane / R. M. Steinman, I. S. Mellman, W. A. Muller [et al.] // J Cell Biol. – 1983. – No.96. – P. 1–27.
    • Тoews G. B. The relationship of inoculum size to lung bacterial clearance and phagocytic cell response in mice / G. B. Тoews, G. N. Gross, A. K. Pierce. // Am. Rev. Respir. Dis. – 1979. – No.120. – P. 559–566.
    • West A. P. TLR signalling augments macrophage bactericidal activity through mitochondrial ROS / A. P. West, I. E. Brodsky, C. Rahner [et al.]. // Nature. – 2011. – No.472. – P. 476–480.
    Publication of the article «World of Medicine and Biology» №2(60), 2017 year, 172-174 pages, index UDK 616.24-002-092.4/.9-07:616.155.3-008.13-07