EnglishУкраїнська
  • Main
  • Useful links
  • Information for Contributors
  • About
  • Editorial board

  • Article
    Belikova O.I., Frenkel Yu.D., Cherno V.S., Kostenko V.O.

    INFLUENCE OF NUCLEAR FACTOR κB INHIBITOR ON BIOCHEMICAL MARKERS OF INSULIN RESISTANCE SYNDROME UNDER HYPOPINEALISM AND HIGH-CALORIE CARBOHYDRATE-LIPID DIET


    About the author: Belikova O.I., Frenkel Yu.D., Cherno V.S., Kostenko V.O.
    Heading EXPERIMENTAL MEDICINE
    Type of article Scentific article
    Annotation The aim of the work is to clarify the effect of the nuclear factor κB (NF-κB) inhibitor on the biochemical markers of the insulin resistance syndrome under hypopnealism induced by the exposure to round-the-clock light, and the maintenance of high-calorie carbohydrate-lipid (HCHL) diet. Material and methods. The research was conducted on 21 white rats of the Wistar line weighing 215-255 g in 3 series of experiments: the first series was designed to obtain necessary indices in intact animals (control series), in the second series we measured the same indices in the rats subject to modeled syndrome of IR (HCHL diet for 60 days and round-the-clock light with intensity of 1500 lux for the last 30 days of the experiment); in the third series we assessed the indices under administration of ammonium pyrrolidinedithiocarbamate (PDTC - ammonium pyrrolidinedithiocarbamate) in a dose of 76 mg/kg 3 times a week starting on the 30th day of the experiment. The concentration of melatonin, insulin and tumor necrosis factor-α (TNF-α) by enzyme-linked immunosorbent assay, glucose, total cholesterol (CHL), triacylglycerol (TAG), and high-density lipoprotein cholesterol (CHL-HDL), ceruloplasmin, were determined. We calculated the content of low and very low density lipoprotein cholesterol (CHL-LDL and CHL-VLDL) by the Friedwald formula, Homeostasis Model Assessment of Insulin Resistance (HOMA-IR). Results. Round-the-clock lightening of the with an intensity of 1500 lux during the period of maintaining the carbohydrate-lipid diet reduces the content of melatonin in the blood serum by 77.7% (p<0.001), indicating the development of experimental hypopneinalism. The administration of the NF-κB inhibitor PDTC against round-the-clock light and the administration of HCHL diet to rats reduces glucose concentration in serum, which is 35.3% (p<0.001) inferior to that in the second series. Under these conditions, the serum insulin concentration and the HOMA-IR index are reduced by 56.5% (p<0.001) and 71.9% (p<0.01), respectively, compared with the data from the second series. The administration of the NF-κB inhibitor PDTC against round-the-clock light and administration of HCHL diet does not significantly affect the concentration of total CHL and CHL-LDL, but significantly increases the CHL-HDL content, which is by 95.8% (p <0.001) higher than the findings in the second series. Under these conditions, the concentration of CHL-VLDL and TAG decreases by 38.3% (p<0.001) and 38.9% (p<0.001) respectively, compared to the findings of the second series. The administration of the PDTC under round-the-clock light and maintaining carbohydrate-lipid diets in rats reduces the concentration of TNF-α and ceruloplasmin by 46.3% (p<0.001) and 33.0% (p<0.01) respectively compared to the findings of the second series. Conclusion. The introduction of the NF-κB inhibitor ammonium pyrrolidinediothiocarbamate in rats under the round-the-clock light and administration of carbohydrate-lipid diet reduces glucose serum concentration, restricts hyperinsulinemia and insulin resistance, reduces the manifestations of dyslipoproteinemia, hypo-α-lipoproteinemia and hypertriacylglycerolemia, as well as inhibits the development of systemic inflammatory responses.
    Tags insulin resistance syndrome, hypopinealism, nuclear factor κB, carbohydrate and lipid metabolism, systemic inflammatory response
    Bibliography
    • Berkalo L. V. Metody klinichnykh ta eksperymentalnykh doslidzhen v medytsyni / L. V. Berkalo, O. V. Bobovych, N. O. Bobrova [ta in.]; Za red. I.P. Kaidasheva. – Poltava, - 2003. – 320 s.
    • Kuzminova I. A. Dinamika izmeneniy lipidnogo spektra krovi v usloviyah eksperimentalnogo gipopinealizma / I. A. Kuzminova, L. A. Bondarenko // Probl. endokrin. patologIYi. - 2006. – No.4. - S.49-55.
    • Kaidashev I. P. Aktyvatsiia NF-κB pry metabolichnomu syndromi / I. P. Kaidashev // Fiziol. zhurn. – 2012. – T.58, №1. – S. 93-101.
    • Rapoport S. I. Melatonin i insulinorezistentnost / S. I. Rapoport, A. Yu. Molchanov, V. A. Golichenkov [i dr.] // Klin. meditsina. – 2013. – No.11. – S. 8-14.
    • Talash V. V. Vplyv inhibitoriv aktyvatsii yadernoho faktora κB na metabolizm i hemokoahuliatsiiu za umov vidtvorennia metabolichnoho syndromu / V. V. Talash, V. O. Kostenko // Farmakolohiia ta likarska toksykolohiia. – 2015. – No. 2. – S. 83-89.
    • Gao Z. Serine phosphorylation of insulin receptor substrate 1 by inhibitor kappa B kinase complex / Z. Gao, D. Hwang, F. Bataille [et al.] // J. Biol. Chem. – 2002. – Vol. 277. – P. 48115-48121.
    • Tornatore L. The nuclear factor kappa B signaling pathway: integrating metabolism with inflammation / L. Tornatore, A.K. Thotakura, J. Bennett [et al.] // Trends Cell Biol. – 2012. – Vol. 22, № 11. – P. 557-566.
    • Qin J.D. Effect of ammonium pyrrolidine dithiocarbamate (PDTC) on NF-κB activation and CYP2E1 content of rats with immunological liver injury / J.D. Qin, Z.H. Cao, X.F. Li [et al.] // Pharm. Biol. – 2014. – Vol.52, №11. – P. 1460-1466.
    Publication of the article «World of Medicine and Biology» №3(61), 2017 year, 080-082 pages, index UDK 616–008.9–07:577.17:[616.43:616.831–675.874.25]
    DOI 10.26724/2079-8334-2017-3-61-80-82