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    Sulaieva O. N.

    OBTAINING OF PLATELET RICH PLASMA: MYTHS AND REALITY


    About the author: Sulaieva O. N.
    Heading EXPERIMENTAL MEDICINE
    Type of article Scentific article
    Annotation A comparative analysis of plasma obtained by using different medical devices for PRP-therapy (RegenLab, Plasmolifting, BTI, MM Medic, Ycellbio tubes) was performed. Plasma was obtained according to the protocols of manufacturers from the blood of 12 volunteers (6 men and 6 women, the average age was 40 ± 0.8 years). Evaluation of the platelet count was performed using a haematological analyser Micro-CC 20 (HTI CША), as well as by flow cytometry using fluorescently labelled antibodies to CD61 (Beckman Coulter, USA). The maximum number of platelets was obtained using Ycellbio tubes, but the drawback of this type of tubes is the need for additional manipulations and openness of the system. The use of tubes containing separating gel (RegenLab, Plasmolifting) does not allow obtaining PRP. The low count of platelets in the obtained plasma may be due to adsorption by separating gel and/or because of use of a more stringent centrifugation regime. The best device for obtaining plasma, that corresponds to requirements to PRP, was a kind of simple tubes (BTI and MM Medic).
    Tags platelet rich platelet, PRP-therapy, platelets
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    Publication of the article «World of Medicine and Biology» №3(61), 2017 year, 150-153 pages, index UDK 611.018.52-54:612.118
    DOI 10.26724/2079-8334-2017-3-61-150-153