| About the author: |
Kocherha Z. R., Shvets L. S., Kosylo N. V. |
| Heading |
EXPERIMENTAL MEDICINE |
| Type of article |
Scentific article |
| Annotation |
The purpose of our research was to study protein peroxidation and enzyme activity of glutathione system in newborns under the influence of ecotoxicants. Primarily, by means of comprehensive assessment of anthropogenic pollution the following four districts of Ivano-Frankivsk region were chosen: Kosiv district (relatively ecologically clean), chemically contaminated Kalush and Burshtyn, and Sniatyn district (the area of intensive radiological control); the increased levels of xenobiotics were found in water, soil and air. We have carried out the investigation of protein peroxidation and enzyme activity of glutathione system in 108 newborns from the above mentioned residence areas. Neutral aldehyde- and ketone-derivatives prevailed in neonates from Burshtyn. Basic aldehyde- and ketone-derivatives with maximum absorption at a wavelength of 530 nm prevailed in newborns from Kosiv district. Study of glutathione system enzymes showed decreasing activity of glutathione peroxidase, glutathione reductase and glutathione-S-transferase in neonates from Burshtyn and Kalush that proves the exhaustion of adaptation mechanisms in terms of a long-term impact of xenobiotics on the fetus’ organism. The obtained results may indicate better functioning of protective anti-radical systems in babies from ecologically friendly zones of Kosiv district. |
| Tags |
newborns, ecological zone, protein peroxidation, enzymes of glutathione system |
| Bibliography |
- Artamonova O. V. Bioetyka eksperymentuvannia nad zhyvymy istotamy. / O. V. Artamonova // Bezpeka zhyttiediialnosti – 2005. – No. 10. – S. 45–48.
- Beletskaya E. N Individualnaya biokorrektsiya ekologozavisimyih sostoyaniy u kriticheskih grupp naseleniya / E. N. Beletskaya, N. M. Onul, V. I. Glavatskaya [i dr.] // Gigiena i sanitariya. – 2014. – No. 2. – S. 34–37.
- Vlasova S. N. Aktivnost glutationzavisimyih fermentov eritrotsitov pri hronicheskih zabolevaniyah pecheni u detey / S. N. Vlasova, E. I. Shabunina, I. A. Pereslyagina // Lab. delo. – 1990. – No.8. – S. 19–21.
- Gorovenko N. G.Osobennosti polimorfizma genov glutation-S- transferazyi u novorozhdennyih razlichnyih ekologicheskih regionov Ivano-Frankovskoy oblasti Ukrainyi / N. G. Gorovenko, S. V. Podolskaya, Z. R. Kocherga // European Jornal of Medcine. – 2014 – Vol.3, No. 1 – P. 17–28.
- Dubynina O. Yu. Okysniuvalnyi stres i okysliuvalna modyfikatsiia bilkiv / O. Yu. Dubynina // Med. khimiia. –2001. – T. 3, No. 2. – S. 5–12.
- Denysenko O. I. Osoblyvosti tsyrkadnykh rytmiv pokaznykiv oksydantnoi ta antyoksydantnoi system krovi u khvorykh na alerhodermatozy / O. I. Denysenko // Dermatolohiia ta venerolohiia. – 2003. – No. 1. – S. 61–64.
- Kuzmak I. P. Okysniuvalna modyfikatsiia bilkiv u shchuriv riznoho viku za umov hostroho otruiennia toksynamy blidoi pohanky / I. P. Kuzmak, Ie. B. Dmukhalska, S. R. Pidruchna [ ta in.] // Medychna ta klinichna khimiia. - 2017. – T. 19, № 1. – S.114–118.
- Melnychuk A. S. Pokaznyky okysnoi modyfikatsii bilkiv ta antyoksydantnoho zakhystu u rotovii ridyni khvorykh na heneralizovanyi parodontyt z chastkovoiu vtratoiu zubiv / A. S. Melnychuk, M. M. Rozhko, H. M. Ersteniuk // Novyny stomatolohii. – 2012. – No. 4 – S. 96–98..
- Rukovodstvo po kratkosrochnyim testam dlya vyiyavleniya mutagennyih i kantserogennyih himicheskih veschestv. Gigienicheskie kriterii sostoyaniya okruzhayuschey sredyi 51. VOZ, Zheneva, - 1989. – 212 s.
- Spitsyin V. A. Ekologicheskaya genetika cheloveka. Nasledstvennyie bolezni: natsionalnoe rukovodstvo / V. A.Spitsyin // – M.: GEOTAR. – 2012. – S.244–283
- Ayyappan S. Antioxidant status in neonatal jaundice before and after phototherapy. / S. Ayyappan, J. Sachu Philip, N. Bharathy // Journal of Pharmacy and Bioallied Sciences. – 2015.– Vol. 7(1). – P.16–21.
- Christiansen L. A longitudinal study of the effect of GSTT1 and GSTM1 gene copy number on survival. / L. Christiansen, C. Brasch-Andersen, L. Bathum [et al.] // Mech Ageing Dev. – 2006. – Vol. 127. № 7 – P. 597–599.
- Correlation between glutathione S-transferase Mu 1 (GSTM1) and glutathione S-transferase pi gene (GSTP1) polymorphisms and markers of inflammatory stress in pregnant females / Poovendhree Reddy, Rajen N. Naidoo, Anil Chuturgoon [et al.] // Journal of Toxicology and Environmental Health Sciences. – 2013. – Vol. 6 (3).– P. 52–59.
- Dusinska M. Are glutathione S transferases involved in DNA damage signalling? Interactions with DNA damage and repair revealed from molecular epidemiology studies / M. Dusinska, M. Staruchova, A. Horska [et al.] // Mutation Research. – 2012. – Vol. 736 (1-2). – P. 130–137.
- Hayes J. D. Glutathione Transferases / J. D. Hayes, J. U. Flanagan, I. R. Jowsey // Ann Rev Pharm Toxicol. – 2005. – Vol. 45. – P. 51–88.
- Kocherha Z. R. State of protein peroxidation in newborns with intrauterine growth restriction / Z. R. Kocherha, H. M. Ersteniuk, L. Ye. Kovalchuk // The Pharma Innovation Jornal. –2014. – Vol. 3. № 6. – P. 9.
- Oxidative Stress and Antioxidant Defense / Birben Esra, Sahiner Umit Murat, Cansin Sackesen [et al.] // World Allergy Organization Journal. –2012. – Vol. 5 (1). – P. 9–19.
- Ozsurekci Ya. Oxidative Stress Related Diseases in Newborns. / Ya. Ozsurekci, A. Kubra // Oxidative Medicine and Cellular Longevity. – 2016. – Review: Article ID 2768365. doi:10.1155/2016/2768365
- Pisochi A. M. The role of antioxidants in the chemistry of oxidative stress: A review. / A. M. Pisochi, A. Pop // European Journal of Medicinal Chemistry. – 2015. – Vol. 5 (97). – P.55–74.
- Samir N. The Role of genetic Polymorphisms in Enviromental Health / N. Samir, I. Kelada, L. David [et al.] // Еnviron Health Perspect. – 2003. – № 111. – P. 1055–1064.
- Waggiallah H. The effect of oxidative stress on human red cells glutathione peroxidase, glutathione reductase level, and prevalence of anemia among diabetics / H. Waggiallah, M. Alzohairy // North American Journal of Medical Sciences. – 2011. – Vol. 3(7) – P. 344–347.
|
| Publication of the article |
«World of Medicine and Biology» №4(62), 2017 year, 139-144 pages, index UDK 612.398 + 612.015.1 + 613.952 + 574.2 |
| DOI |
10.26724/2079-8334-2017-4-62-139-144 |
