| About the author: |
Lykhatskyi P.H., Fira L.S. |
| Heading |
EXPERIMENTAL MEDICINE |
| Type of article |
Scentific article |
| Annotation |
The aim of this work was to investigate the activity of the NO-synthase system and the development of inflammatory processes in rats of different ages in the dynamics of tobacco smoke poisoning. Studies were carried out on white, non-native male rats of different ages, which were exposed to tobacco smoke daily for 45 days in a sealed chamber (exposure time 6 min). After 15, 30 and 45 days from the onset of the animals' defeat with tobacco smoke, they were taken out of the experiment by euthanizing them under thiopental anesthesia, observing all the rules for working with vertebrates. It was found that the damage to the rats for 45 days with tobacco smoke led to an increase in the serum content of the proinflammatory cytokine IL-6. The most sensitive to this toxicant were immature rats, in which the content of IL-6 increased by 50%, 164% and 237%, respectively, 15, 30 and 45 days after injury. Poisoning of rats with tobacco smoke led to an increase in the activity of inducible NO-synthase in serum and liver throughout the study period. It is noted that the body of immature rats reacts most quickly and most strongly to tobacco smoke. The activity of iNO synthase reached the highest values both in the blood serum and in the liver of immature animals. After the defeat of rats with tobacco smoke, in our experiments a decrease in the activity of endothelial NO synthase in all age groups of rats was noted. In immature rats, the activity of eNOS in serum decreased 1.4 times, 1.8 and 2 times in 15, 30 and 45 days, respectively, compared with the norm. In the liver of rats of all age groups, during the experiment, a similar decrease in eNOS activity was observed and the lowest value was reached at the last term of the study (day 45 of the exposure to tobacco smoke). |
| Tags |
tobacco smoke, white rats, nitrooxidative stress, NO synthase, interleukins |
| Bibliography |
- Aleksandrova Yu. N. O sisteme tsitokinov / Yu. N. Aleksandrova // Pediatriya. – 2007. – No. 3 (86). – S. 124–28.
- Berezhnaya N. M. Tsitokinovaya regulyatsiya pri patologii: stremitelnoye razvitiye i neizbezhnyye voprosy / N. M. Berezhnaya // Tsitokiny i vospaleniye. - 2007. - No. 2 (6). - S. 26-34.
- Mykhaylenko V.M. Markery nitrozatyvnoho stresu pry inhalyatsiyniy diyi oksydiv azotu v normi ta pry pukhlynnomu rosti / V. M. Mykhaylenko, P. M. Mykhaylenko, I. S. Holovina // Suchasni problemy toksykolohiyi. – 2011. – No.4. – S. 28-35.
- Pikas O.B. Pro stan kurinnya tsyharok u suchasnykh umovakh, yoho vplyv na vynyknennya zakhvoryuvan v orhanizmi lyudyny / O. B. Pikas // Bukovynskyy medychnyy visnyk. – 2015. – No. 4 (76). – S. 227-30.
- Yachnyk A. I. Fiziolohichni aspekty oksydu azotu pry porushennyakh lehenevoho krovoobihu ta rolʹ L-arhininu v korektsiyi yoho syntezu / A. I. Yachnyk, M. I. Humenyuk, A. D. Chopchyk // Ukrayinskyy pulmonolohichnyy zhurnal. – 2008. – No. 1. – S.40-44.
- Argacha J. Acute effect of sidestream cigarette smoke extract on vascular endothelial function / J. Argacha, D. Fontaine, D. Adamopoulos, A. Ajose, P. Van de Borne [et.al.] // J. Cardiovasc. Pharmacol. – 2008. – Vol. 52(3). – Р. 262-27.
- Birben E. Oxidative Stress and Antioxidant Defense / E.Birben, U. Sahiner, C. Sackesen, S. Erzurum, O. Kalayci // World Allergy Organ. J. – 2012. – Vol. 5(1). – Р. 9–19.
- Csiszar A. Oxidative stress and accelerated vascular aging: implications for cigarette smoking / A. Csiszar, A. Podlutsky, M. Wolin, G. Losonczy, P. Pacher [et.al.] // Front Biosci (Landmark Ed). – 2009. – Vol. 14(1). – Р. 3128-44.
- Gross D. Ethics in Animal-Based Research / D. Gross, R. Tolba // Eur. Surg. Res. – 2015. – Vol. 55(1-2). – Р. 43 – 57.
- Lee J. Cigarette smoking and inflammation: cellular and molecular mechanisms / J. Lee, V. Taneja, R. Vassallo // J. Dent. Res. – 2012. – Vol. 91(2). – Р. 142-49.
- Luckett-Chastain L. Interleukin (IL)-6 modulates transforming growth factor-beta expression in skin and dermal fibroblasts from IL-6- deficient mice / L. Luckett-Chastain, R. Gallucci // Br. J. Dermatol. – 2009. – Vol. 161(2). – Р. 237–48.
- Nam J. Interleukin-13/-4-induced oxidative stress contributes to death of hippocampal neurons in aβ1-42-treated hippocampus in vivo / J. Nam, K. Park, E. Park, Y. Lee, H. Lee [et.al.] //Antioxid. Redox. Signal. – 2012. – Vol. 16(12). – Р. 1369-83.
- Nolan Y. Role of interleukin-4 in regulation of age-related inflammatory changes in the hippocampus / Y. Nolan, F. Maher, D. Martin, R. Clarke, M. Brady [et.al.] // J. Biol. Chem. – 2005. – Vol. 280(10). – Р. 9354–62.
- Okeh U. Statistical problems in medical research / U. Okeh // East. Afr. J. Public. Health. – 2009. – Vol. 6(1). – Р. 1 – 7.
- Rahman M. Structural and functional alteration of blood vessels caused by cigarette smoking: an overview of molecular mechanisms / M. Rahman, I. Laher // Curr. Vasc. Pharmacol. – 2007. – Vol. 5(4). – Р. 276-92.
- Wirth N. Respiratory diseases related to passive smoking / N. Wirth, A. Bohadana, A. Spinosa, Y. Martinet // Rev. Mal. Respir. – 2009. – Vol. 26(6). – P. 667-78.
- Zhou H. Changes in serum contents of interleukin-6 and interleukin-10 and their relation with occurrence of sepsis and prognosis of severely burned patients / J. Zhou, J. Tu, Y. Huangetal. // Zhonghua Shao Shang Za Zhi. – 2012. – Vol. 28(2). – P. 111-15.
|
| Publication of the article |
«World of Medicine and Biology» №4(62), 2017 year, 145-149 pages, index UDK 599.32:612.76+615.063-002:515.9]-092.4 |
| DOI |
10.26724/2079-8334-2017-4-62-145-149 |
