English Українська
  • Main
  • Useful links
  • Information for Contributors
  • About
  • Editorial board

    •
    Article
    World of Medicine and Biology / №1(63), 2018 / INDICATORS CELL CYCLE AND DNA FRAGMENTATION IN CELLS OF SMALL INTESTINE MUCOSA 14, 21 AND 30 DAYS AFTER SKIN BURNS ON THE BACKGROUND OF PRE-LIMINARY INFUSION OF SOLUTION LACTOPROTEIN WITH SORBITOL OR HAES-LX 5%
    A. O. Gavryluk, G. M. Galunko, I. L. Chereshniuk, V. O. Tikholaz, E. V. Cherkasov, І. V. Dzevulska, О. І. Kovalchuk

    INDICATORS CELL CYCLE AND DNA FRAGMENTATION IN CELLS OF SMALL INTESTINE MUCOSA 14, 21 AND 30 DAYS AFTER SKIN BURNS ON THE BACKGROUND OF PRE-LIMINARY INFUSION OF SOLUTION LACTOPROTEIN WITH SORBITOL OR HAES-LX 5%

    About the author: A. O. Gavryluk, G. M. Galunko, I. L. Chereshniuk, V. O. Tikholaz, E. V. Cherkasov, І. V. Dzevulska, О. І. Kovalchuk
    Heading EXPERIMENTAL MEDICINE
    Type of article Scentific article
    Annotation In analyzing the data of cell cycle and DNA fragmentation of the cells of the mucous membranes of the small intestine of rats in the late stages after the thermal burn of skin 2-3 degrees, in the area of 21-23% of the body surface, on the background of the previous use of infusion solutions, it was found that "lactoprotein with sorbitol" or HAES-LX-5% have a positive effect on cell cycle performance: after 14 days, the S-phase data and the index of proliferation were increased compared with those in the burn group + 0.9% NaCl solution in the same period; after 21 days, the S-phase data and the index of proliferation of these two groups were significantly higher than those in the burns + 0.9% NaCl solution, and at the same time, the values of the SUB-G0G1 interval in both groups were lower than those in the group where was used 0,9% NaCl solution on the background of burn. After 30 days in the burn + HAES-LX-5% group, all cell cycle indices have no significant or trend-specific differences compared to those in the non-burning group, and with "lactoprotein with sorbitol", the G0G1 and the proliferation phases have been significantly lower than indicators in a group without skin burns.
    Tags cell cycle, DNA cytometry, small intestine, rats, thermal burn skin, "lactoprotein with sorbitol", HAES-LX 5%
    Bibliography
    • Béchir M, Puhan MA, Fasshauer M, Schuepbach RA, Stocker R, Neff TA. Early fluid resuscitation with hydroxyethyl starch 130/0.4 (6%) in severe burn injury: a randomized, controlled, double-blind clinical trial. Crit. Care, 2013; 17(6): R299.
    • Carter EA, Udall JN, Kirkham SE, Walker WA. Thermal injury and gastrointestinal function. I. Small intestine nutrient absorption and DNA synthesis. J. Burn. Care. Rehab., 1986; 7: 469-474.
    • Cherkasov EV. Strukturni zminy` ty`musa pry` ekspery`mental`nij opikovij xvorobi u shhuriv za umov yiyi likuvannya shlyaxom vnutrishn`ovennoyi infuziyi HAES-LX-5%. Ukrayins`ky`j medy`chny`j al`manax, 2012; 15(3): 225-230. (in Ukraine)
    • Clark JA, Coopersmith CM. Intestinal crosstalk – a new paradigm for understanding the gut as the “motor” of critical illness. Shock (Augusta, Ga), 2007; 28(4): 384-393.
    • De Jong PR, González-Navajas JM, Jansen NJG. The digestive tract as the origin of systemic inflammation. Crit.Care,2016; 20(1): 279.
    • Earley ZM, Akhtar S, Green SJ, Naqib A, Khan O, Cannon AR, Choudhry MA. Burn Injury Alters the Intestinal Microbiome and Increases Gut Permeability and Bacterial Translocation. PLoS ONE, 2015; 10(7): e0129996.
    • Gavrylyuk AO, Galunko GM, Volkov AO, Shapoval OM. Gistologichni zminy v tonkij kyshci v pizni stadiyi opikovoyi xvoroby pry korekciyi infuzijny`my` rozchy`namy`. Visnyk morfologiyi, 2017; 23(2): 226-231. (in Ukraine)
    • Grimes L, Doyle A, Miller AL, Pyles RB, Olah G, Szabo C. Intraluminal Flagellin Differentially Contributes to Gut Dysbiosis and Systemic Inflammation following Burn Injury. PLoS ONE, 2016; 11(12): e0166770.
    • Gunas I, Dovgan I, Masur O. Method of thermal burn trauma correction by means of cryoinfluence. 1997; Abstracts are presented in zusammen mit der Polish Anatomical Society with the participation of the Association des Anatomistes Verhandlungen der Anatomischen Gesellschaft, Olsztyn (р. 105). Jena – München : Der Urban & Fischer Verlag.
    • Gunas IV, Kondraczkyj BO, Nurmetova IK, Dzevulska IV, Kovalchuk OI, Cherkasov YeV. Dynamika zmin rivnya endogennoyi intoksykaciyi v organizmi shhuriv protyagom misyacya pislya opiku shkiry II-III stupenya, ploshheyu 21-23% poverxni tila ta yiyi korekciya infuzijnymy rozchynamy, laktoproteyinom z sorbitolom ta HAES-LX-5 %. Ukrayinskyj morfologichnyj almanax, 2012; 10(4): 29-34. (in Ukraine)
    • Ikeda H, Suzuki Y, Suzuki M, Koike M, Tamura T, Tong J. Apoptosis is a major mode of cell death caused by ischaemia and ischaemia/reperfusion injury to the rat intestinal epithelium. Gut, 1998; 42: 530-537.
    • Ismailova LI, Guterova LD. Rol kishechnoy mikrofloryi v patogeneze ozhogovoy bolezni. Zdravoohranenie Tadzhikstana, 2008; 5: 97-98. (in Russian)
    • Jeschke G, Gauglitz GG, Kulp GA, Finnerty CC, Williams FN, Kraft R. Long-Term Persistance of the Pathophysiologic Response to Severe Burn Injury. PLoS ONE, 2011; 6(7): e21245.
    • Kozhemyakin YuM, Xromov OS, Boldyryeva NU, Dobrelya NV, Sajfetdinova GA. Naukovo-praktychni rekomendaciyi z utrymannya laboratornyx tvaryn ta roboty z nymy. 2017; K. : Interservis. (in Ukraine)
    • Lee MO. Determination of the surface area of the white rat with its application to the expression of metabolic results. Am. J. Physiol., 1989; 24: 1223.
    • Makarova OI, Chajkovskyj YuB. Osoblyvosti ul`trastrukturnyx zmin v respiratornomu viddili legen shhuriv u viddalenyj period pislya termichnoyi travmy za umov yiyi korekciyi koloyidno-giperosmolyarnym infuzijnym rozchynom HAES-LX-5%. Svit medycyny`ta biologiyi, 2014; 4(46): 115-120. (in Ukraine)
    • Meylanova RD, Magomedov MA. Otsenka mikrotsirkulyatornogo rusla bryizheyki tonkoy kishki kryis metodom vitalnoy mikroskopii pri korrektsii termicheskogo ozhogovogo shoka perftoranom.Sovremennyie naukoemkie tehnologii,2005;6:37-38.(in Russian)
    • Regas FC, Ehrlich HP. Elucidating the vascular response to burns with a new rat model. J. Trauma, 1992; 32(5): 557-563.
    • Semenenko AI, Kondraczkyj BO, Yakovlyeva OO, Sheremeta AV, Xodakivska OL, Petrova GD. Vplyv laktoproteyinu z sorbitolom ta HAES-LX-5% na dynamiku deyakyx pokaznykiv funkcionuvannya pechinky pry opikovij xvorobi u shhuriv. Visnyk morfologiyi, 2010; 16(2): 363-365. (in Ukraine)
    • Shano VP, Grin VK, Fistal EYa, Miminoshvili OI, Zayats YuV. Ozhogovyiy shok. 2006; Donetsk : Yugo-Vostok. (in Russian)
    • Stefanov OV. Doklinichni doslidzhennya likarskih zasobiv. Metodichni rekomendatsiyi. 2001; Kiyiv: Avitsena. (in Ukraine)
    • Szczesny B, Brunyánszki A, Ahmad A, Oláh G, Porter C, Toliver-Kinsky T. Time-Dependent and Organ-Specific Changes in Mitochondrial Function, Mitochondrial DNA Integrity, Oxidative Stress and Mononuclear Cell Infiltration in a Mouse Model of Burn Injury. PLoS ONE, 2015; 10(12): е0143730.
    • Yalcin AD, Bisgin A, Erbay RH, Oguz O, Demir S, Yilmaz M. Trimetazidine effect on burn-induced intestinal mucosal injury and kidney damage in rats. International Journal of Burns and Trauma, 2012; 2(2): 110-117.
    Publication of the article «World of Medicine and Biology» №1(63), 2018 year, 104-108 pages, index UDK (616.341:611.018.1):616.5-001.37
    DOI 10.26724/2079-8334-2017-4-62-104-108