| About the author: |
I.V. Gunas, Yu.I. Guminskiy, N.P. Ocheretn, D.A. Lysenko, О.І. Kovalchuk, І.V. Dzevulska, E.V. Cherkasov |
| Heading |
EXPERIMENTAL MEDICINE |
| Type of article |
Scentific article |
| Annotation |
In the experiment, during 7 days, changes in cell cycle and DNA fragmentation of spleen cells in rats after burn injuries were studied in the background of the introduction of 0.9% NaCl solution. After skin burn in the background of the introduction of 0.9% NaCl solution, after 1 day, greater values of the interval SUB-G0G1 and the G0G1 phase and, at the same time, lower values of the phases S, G2+M and the index of proliferation were determined, indicating the pathological induction of apoptosis and violations of the synthetic processes of splenocytes. After 3 days after burning the skin, large average values of the G0G1, proliferation and proliferation indexes were determined and, at the same time, the highest possible apoptosis rate compared to similar animal numbers in the 1 day after burn, which could be considered as the activation of mechanisms for compensating for pathological effects thermal damage at the given time. 7 days after skin burn, the average values of the G0G1 and the proliferation index are close to the similar figures for a group of animals without burn injuries of the skin, with the introduction of 0.9% NaCl solution, while the values of the S-phase (at almost 2.5 times) and the SUB-G0G1 interval (2.7 times), indicating insufficient compensation for the proliferative activity of the spleen cells against the background of increased apoptosis |
| Tags |
cell cycle indexes, DNA fragmentation, spleen, rats, burn skin, 0.9% NaCl solution |
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| Publication of the article |
«World of Medicine and Biology» №1(63), 2018 year, 116-120 pages, index UDK 616.411+616.5-001.37 |
| DOI |
10.26.724/2079-8334-2018-1-63-116-120 |
