Dmytryshyn T.M.

ANALYSIS OF CORRELATIONS BETWEEN MICROBIOLOGICAL AND BIOCHEMICAL, BIOPHYSICAL PARAMETERS IN PATIENTS WHO USE REMOVABLE DENTURES AND HAVE DIFFERENT LEVELS OF ORAL HYGIENE

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About the author:	Dmytryshyn T.M.
Heading	CLINICAL MEDICINE
Type of article	Scentific article
Annotation	Despite a large number of studies on the choice of oral hygiene products and methods for persons using removable dentures, there is limited information on the study of the relationship between microbiological and biochemical, biophysical indicators of oral fluid in such individuals. Objective: to establish the relationship between microbiological, biochemical, and biophysical parameters in patients who use partial and complete removable dentures and have different levels of oral hygiene. Materials and methods. To achieving objective were examined 227 patients, who used partial and complete removable dentures and had different levels of oral hygiene. The state of oral hygiene in persons using removable structures of dentures was determined by the integral index of oral hygiene (IIOH). Microbiological parameters were determined in all patients: the massiveness of colonization with б-hemolytic Streptococcus sp., the total mass of the colonization of the prosthetic bed mucosa by pathogenic microorganisms, as well with parameters, proposed by us: microbial number (MN) and microbial number deficiency (DMN). The conducted biochemical studies of oral fluid included concentrations of glucose, lactate, urea, ammonia. The concentration of mucin in the oral liquid was determined by the method of E.N. Korobeynikova, E.I. Ilyinych using Benedict reagent. The viscosity of the oral fluid was determined using standard micropipette with T. L. Redinovaya method. The salivation speed was calculated in ml / min, according to the formula: amount of extracted oral liquid / time of collection. Indicator of concentration of hydrogen ions pH was determined using pH-meter-millivoltmeter pH-410 «NKPA Akvilon» (RF). Research results. Patients, who use removable dentures and have different levels of oral hygiene have established a close relationship between some microbiological and biochemical, biophysical indicators. Thus, for the values of the index IIOH≤ 44,0 (proper level of hygiene) the reliable average, strong in magnitude and direct in the direction correlation connection was established between the indicators: MN and concentration of ammonia, hydrogen ions (pH); DMN and salivation speed, mucin concentration; the massiveness of colonization by the most typical representatives of the resident microflora of the oral cavity - α-hemolytic Streptococcus sp. and the salivation speed; the total massiveness of the colonization of pathogenic microflora and the viscosity of the oral fluid. For the same value of IIOH, the strong, average inverse correlation is established between the massiveness of colonization by α-hemolytic Streptococcus sp. and the concentration of ammonia, pH, lactate of the oral fluid. Among patients, who had the IIOH index > 44,0 (improper hygiene) a reliable average, strong in magnitude, and a straight line, the correlation relationship was established between the indices: MN and viscosity, pH; DMN and salivation speed; massiveness of prosthetic bed mucosa colonization by α-hemolytic streptococci and salivation speed, concentration of urea and glucose. For the same value of IIOH, the strong, average inverse correlation bonds are established between the total massiveness of colonization by pathogenic microflora and glucose concentration; MN and salivation speed; DMN and viscosity, ammonia concentration. Conclusion Thus, the obtained results allowed to clarify the interrelations between the indicators characterizing the state of microflora and biochemical, biophysical indicators of oral fluid for various oral hygiene states in patients using removable dentures.
Tags	microbiological and biochemical, biophysical parameters, oral cavity hygiene, removable plate dentures
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Publication of the article	«World of Medicine and Biology» №2(64), 2018 year, 044-048 pages, index UDK 616-071+616.314-083+616.314-77
DOI	10.26724/2079-8334-2018-2-64-44-48