| About the author: |
M.M.Koptev, N.І.Vynnyk, O.V.Kokovska, B.M.Filenko, S.M.Bilash |
| Heading |
EXPERIMENTAL MEDICINE |
| Type of article |
Scentific article |
| Annotation |
The perfect knowledge of the microscopic structure of the appropriate organ is crucial in the solution of many urgent problems of clinical medicine. Better preservation of tissue structures is achieved when the tissues are embedded into epoxy resins followed up with the semi-thin section making. In contrast to the thick sections that are used for light microscopy, due to the greater awareness and relative cost effectiveness without the need of complicated expensive equipment, the method of semi-thin sections today is currently used in morphological studies. The paper was aimed at the study of the prospects of using the semi-thin section method to evaluate the immobilization stress-induced structural changes in the lungs. The resulting study showed the appropriateness of the use of semi-thin sections to study structural changes in lungs, induced by the experimental stress. The study of the semi-thin sections is the beneficial technique of the morphological analysis that permits to evaluate the structure of the studied material at the tissue and cellular level. |
| Tags |
semi-thin sections, lungs, stress |
| Bibliography |
- Belokon SA, Vitko YuN, Tkachenko PI, Starchenko II, Gryn VG. Optimizatsiya issledovaniya strukturnykh elementov biologicheskikh tkaney na gistograficheskikh shlifakh. Molodoy uchenyy. 2014; (15): 134-7. [in Russian]
- Bilash SM, Shepitko VI, Yeroshenko HA, Kostylenko YuP, vynakhidnyky; Vyshchyi derzhavnyi navchalnyi zaklad Ukrayiny “Ukrayinska medychna stomatolohichna akademiya”, patentovlasnyk. Sposib identyfikatsiyi endokrynotsytiv shlunkovo-kyshkovoho traktu na epoksydnykh shlifakh. Patent Ukrayiny № 81382. 2013 Chervenʹ 25. [in Ukrainian]
- Bilash SM, Shepitko VI, Ivanochko VM, Yeroshenko HA, Bilash VP, vynakhidnyky; Vyshchyi derzhavnyi navchalnyi zaklad Ukrayiny “Ukrayinska medychna stomatolohichna akademiya”, patentovlasnyk. Sposib doslidzhennya morfolohiyi mozochka na epoksydnykh shlifakh. Patent Ukrayiny № 98541. 2015 Sichen 15. [in Ukrainian]
- Zakon Ukrayiny “Pro zakhyst tvaryn vid zhorstokoho povodzhennya” № 3447 – IV vid 21.02.2006. Kyyiv; 2006. 18 s. [in Ukrainian]
- Korzhevskiy DE, Kirik OV, Karpenko MN. Teoreticheskiye osnovy i prakticheskoye primeneniye metodov immunogistokhimii. Sankt Peterburg: SpetsLit; 2012. 112 s. [in Russian]
- Kostylenko YuP, Kovalev YeV. Metody raboty s polutonkimi epoksidnymi srezami v gistologicheskoy praktike. Arkhiv anatomii, gistologii, embriologii. 1978; 75 (12): 68–72. [in Russian]
- Kostylenko YuP, Boyko IV, Starchenko II, Prilutskiy AK. Metod izgotovleniya gistologicheskiy preparatov, ravnotsennykh polutonkim srezam s bolshoy obzornoy poverkhnosti, dlya mnogotselevykh morfologicheskikh issledovaniy. Morfologiya. 2007; (5): 94–6. [in Russian]
- Starchenko II, Vitko YUN. Osobennosti topografii troynichnogo uzla cheloveka vo vnutriutrobnom periode razvitiya. Vísnik problem bíologíí̈ í meditsini. 2013; 98 (1-1): 208–10. [in Russian]
- TkachenkoPI, Starchenko II, Bilokon SO. Pro pokhodzhennya ta morfolohichni vidminnosti shchyto-yazychnoyi i zobno-hlotkovoyi protok yak anatomichnykh utvoren, shcho mozhut staty dzherelom rozvytku kist shyyi. Visnyk problem biolohiyi i medytsyny. 2014; 108 (2-2): 179–82. [in Ukrainian]
- European convention for the protection of vertebrate animals used for experimental and other scientific purposes. Council of Europe, Strasbourg; 1986. 53 p.
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| Publication of the article |
«World of Medicine and Biology» №2(64), 2018 year, 153-156 pages, index UDK 616.24-06:616.45-001.1/3]-091.8:616-076 |
| DOI |
10.26724/2079-8334-2018-2-64-153-156 |
