Kuzmenko L. M., Polishchuk A. A., Usenko S. O., Shostya A. M., Stoyanovskiy V. G., Karpovskiy V. I., Bilash S. M.

PROOXIDANT-ANTIOXIDANT HOMEOSTASIS IN THE TISSUES OF THE UTERUS OF PIG, DEPENDING ON THE PERIODS OF THE REPRODUCTIVE CYCLE

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About the author:	Kuzmenko L. M., Polishchuk A. A., Usenko S. O., Shostya A. M., Stoyanovskiy V. G., Karpovskiy V. I., Bilash S. M.
Heading	BIOLOGY
Type of article	Scentific article
Annotation	The article highlights studying results of disclosure of the peculiarities of formation prooxidant-antioxidant homeostasis in the tissues of the uterus of pig in the different periods of the reproductive cycle and pregnancy. In experiments on the basis of analogies were used 40 clinically healthy pigs of large white breed aged 8 months and weighing 125-130 kg. The pigs were kept in fences, 10-11 animals in each group. Not inseminated and pregnant sows were fed according to the rules taking into account their physiological state. The pigs and pig sows were slaughtered at different periods of the reproductive cycle: luteal phase (10 days after ascertainment of the immobility reflex, estrus (24 hours after the beginning of the estrus), 10, 15, 20, 30, 60 and 90 days of pregnancy. After slaughter, samples of tissues were taken from the sows: the mucous membrane and muscle of the uterus to determine components of prooxidant-antioxidant homeostasis in them. It has been established that the state the prooxidant-antioxidant homeostasis in tissues of the horns of the pig uterus of are labile and depends on the period of reproductive cycle. It was revealed that in the endometrium and myometrium with the beginning of the period of the estrus – the growth of activity catalase (р<0,001), superoxidedismutase and the level of diene conjugates (р<0,01), with increased use ascorbic acid and dehydroascorbic acid, reflecting the strengthening of the intensity passing free radical peroxide oxidation in connection with the insemination process. and the second half of pregnancy. These changes are aimed at providing optimal conditions for insemination and starting of embryo development. It has been discovered that there is a significant inter-tissue differentiation in the formation of prooxidant-antioxidant homeostasis in the endometrium in the uterus of the pigs – higher levels of catalase and non-enzyme antioxidants compared to myometrium in almost all of the study periods of the reproductive cycle. The mucous membrane was characterized by a higher degree of variability of the studied parameters, especially non-enzyme antioxidants, it is caused by the intensive use of them both the mother's body for creation of optimal conditions for the normal embryo development, and embryos during their intensive growth. The mucous membrane at various stages serves as a depot of low antioxidants in the uterus.
Tags	pigs, reproductive cycle, endometrium, myometrium, catalase, ascorbic acid, vitamin A, vitamin E
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Publication of the article	«World of Medicine and Biology» №2(64), 2018 year, 198-203 pages, index UDK 612.014, 636.4.
DOI	10.26724/2079-8334-2018-2-64-198-203