• Main
  • Useful links
  • Information for Contributors
  • About
  • Editorial board

  • Article
    O.Ya. Zhurakivska, Yu.V. Bodnarchuk, V.M. Pertsovych, H.B. Kulynych, V.A. Мiesoiedova


    About the author: O.Ya. Zhurakivska, Yu.V. Bodnarchuk, V.M. Pertsovych, H.B. Kulynych, V.A. Мiesoiedova
    Type of article Scentific article
    Annotation The purpose of the research work was to establish the peculiarities of morphofunctional changes in the liver in the early stages of streptozotocin-induced diabetes mellitus (DM). Diabetes mellitus was induced by a single intraperitoneal injection of streptozotocin (5mg per 100g of body weight). Specimens for the study were collected on the 14th and 28th day of the experiment. Histologic, electron-microscopic, and biochemical methods of investigation were used. The study revealed that in streptozotocin-induced diabetes mellitus with the increase of levels of glucose and glycosylated hemoglobin the granular degeneration of hepatocytes with the transition to vacuolar degeneration takes place. Structural changes in the liver lead to its dysfunction, which is confirmed by the increased levels of specific hepatic aminotransferases and the decreased De Ritis ratio starting from the 14th day of the experiment. Such changes are followed by the increased activity of pro-oxidant system which is confirmed by the significant increase of levels of superoxide dismutase in blood serum and liver homogenates.
    Tags liver, liver transaminase, superoxide dismutase, streptozotocin-induced diabetes mellitus
    • Botros M, Sikaris KE. The de Ritis ration: The test of time. The Clinical Biochemist Reviews. 2013; 34(3):117-130.
    • Godam ET, Samaila MO, Ibegbu AO, et al. Histological and Biochemical Effects of Azadirachta indica and Melatonin in Streptozotocin-induced Diabetic Wistar rats. Annals of Experimental  Biology. 2014; 2(2): 9-22.
    • Hyder MA, Hasan M, Mohieldein AH. Comparative Levels of ALT, AST, ALP and GGT in Liver associated Diseases. European Journal of Experimental Biology. 2013; 3(2):280-4.
    • Ivankiv YI, Oleshchuk OM, Datsko TV, et al. Characteristics of indicators of prooxidant-antioxidant homeostasis, carbohydrate metabolism and morphological changes of the liver under the conditions of melatonin administration to experimental diabetes mellitus type II. Bulletin of Morphology. 2016; 22(2):253-8.
    • Khavrona OP, Fartushok NV, Fedevych YM, et al.  Changes in redox processes and fatty acid composition of lipids in the liver of rats under the conditions of streptozotocin-induced diabetes mellitus. Physiological journal. 2010; 56(6):55-61.
    • Kulitska MI. Functional state of the liver in rats under experimental hepatotoxic syndrome. Medical and clinical chemistry. 2015; 17(4):74-7.
    • Leon ChY, Roberts CK, Crespi CM, et al. Elevated liver enzymes in individuals with diagnosed diabetes in the US. Diabetes Complication. 2013; 27(4):333-9.
    • Levytskyi VA, Zhurakivska OY, Miskiv VA, Zaiats LM, Petriv RB, Yakymiv YM, Kishchuk BM, Hnatiuk RZ. Technique to induce diabetes mellitus type I in animals of varied age. Patent № 62966. Ukraine. Application № u 201101566; applied on Jan.11,2011; dated Sep.20,2011; Bulletin.18: 6.
    • Pitocco CD, Zaccardi F, Stasio ED. Oxidative stress nitric oxide, and diabetes. The Review of Diabetic Studies. 2010;7(1): 15-25.
    • Salih ND, Muslih RK, Hamoodi SR. Histological Liver Changes in Streptozotocin-induced Diabetic Mice. International Medical Journal Malaysia. 2009; 8:10-6.
    Publication of the article «World of Medicine and Biology» №3(65), 2018 year, 152-156 pages, index UDK 611.36+616-092.9+616.379-008.64
    DOI 10.26724/2079-8334-2018-3-65-152-156